Arabidopsis thaliana AtHRS1 gene is involved in the response to Heterodera schachtii infection and its overexpression hampers development of syncytia and involves a jasmonic acid-dependent mechanism

Wiśniewska, Anita; Wojszko, Kamila; Różańska, Elżbieta; Lenarczyk, Klaudia; Sobczak, Mirosław

doi:10.1016/j.jplph.2022.153680

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…Seeds of the T-DNA pp2-a3 and pp2-a8 insertional mutants [SALK_045411 and SALK_027300 for AtPP2-A3 (AT2G26820) and SALK_074249 for AtPP2-A8 (AT5G45070)] were obtained from the Nottingham Arabidopsis Stock Centre (UK). Seeds were sterilized and plants were cultured in vitro on 0.2KNOP or 0.5MS media under conditions described by Wiśniewska et al ( 2022 ).…”

Section: Methodsmentioning

confidence: 99%

The role of AtPP2-A3 and AtPP2-A8 genes encoding Nictaba-related lectin domains in the defense response of Arabidopsis thaliana to Heterodera schachtii

Wojszko

Różańska

Sobczak

et al. 2023

Planta

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Main conclusion Expression levels of AtPP2-A3 and AtPP2-A8 are reduced in syncytia induced by Heterodera schachtii and decline of their expression levels decreases host susceptibility, whereas their overexpression promotes susceptibility to parasite. Abstract Plant-parasitic nematodes cause huge crop losses worldwide. Heterodera schachtii is a sedentary cyst-forming nematode that induces a feeding site called a syncytium via the delivery of secreted chemical substances (effectors) to host cells, which modulate host genes expression and phytohormone regulation patterns. Genes encoding the Nictaba-related lectin domain have been found among the plant genes with downregulated expression during the development of syncytia induced by H. schachtii in Arabidopsis thaliana roots. To investigate the role of two selected Nictaba-related genes in the plant response to beet cyst nematode parasitism, mutants and plants overexpressing AtPP2-A3 or AtPP2-A8 were infected, and promoter activity and protein localization were analyzed. In wild-type plants, AtPP2-A3 and AtPP2-A8 were expressed only in roots, especially in the cortex and rhizodermis. After nematode infection, their expression was switched off in regions surrounding a developing syncytium. Astonishingly, plants overexpressing AtPP2-A3 or AtPP2-A8 were more susceptible to nematode infection than wild-type plants, whereas mutants were less susceptible. Based on these results and changes in AtPP2-A3 and AtPP2-A8 expression patterns after treatments with different stress phytohormones, we postulate that the AtPP2-A3 and AtPP2-A8 genes play important roles in the defense response to beet cyst nematode infection.

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Section: Methodsmentioning

confidence: 99%

The role of AtPP2-A3 and AtPP2-A8 genes encoding Nictaba-related lectin domains in the defense response of Arabidopsis thaliana to Heterodera schachtii

Wojszko

Różańska

Sobczak

et al. 2023

Planta

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“…After equalization of RNA concentrations, cDNA was synthesised using a High-Capacity cDNA Reverse Transcription Kit with the MultiScribe Reverse Transcriptase and random primers (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. The UBP22 was used as a reference gene [ 28 ]. Primers are listed in Table 2 .…”

Section: Methodsmentioning

confidence: 99%

Mutations in Selected ABA-Related Genes Reduce Level of Arabidopsis thaliana Susceptibility to the Beet Cyst Nematode Heterodera schachtii

Różańska

Krępski

Wiśniewska

2023

Plants

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Heterodera schachtii is a common parasite of many important crops such as beets and Brassicaceae (oilseed rape, cabbage or mustard). Arabidopsis thaliana is a model plant also used for studying defence responses to pathogens or pest infections. Defence responses of plants are often regulated and fine-tuned by stress phytohormones: salicylic acid (SA), jasmonic acid (JA), ethylene (Et) and abscisic acid (ABA), of which the role of ABA in these responses is the least examined. The aim of this study was to show, if and which genes related to ABA turnover can be modulated during the development of nematode-induced feeding sites in A. thaliana roots. To answer the question, we performed infection tests on wild type and ABA mutant roots and analysed the expression levels of selected ABA-related genes (ABI1, ABI2, ABI5, PYL5, PYL6, CYP707A1 and CYP707A4) at the early stage of root infection. Our results show that the expression of ABI2, ABI5 (ABA signalling) and CYP707A4 (ABA metabolism) genes was upregulated in feeding sites at 4 dpi, whereas the level of expression of PYL5 and PYL6 (ABA receptors) genes was decreased. Mutations in ABI1, ABI2, ABI5, CYP707A1 or CYP707A4 genes led to a decrease of A. thaliana susceptibility verbalised as the number of fully developed females, whereas mutations in PYL5 or PYL6 genes did not influence the number of females of the nematode. Based on the results, it can be concluded that the modifications of analysed ABA-related gene expression are required for the proper development of nematodes; however, further in-depth analyses are required.

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Deciphering the synergistic effect of Jasmonic acid and Spermine in mitigating root-knot nematode stress in tomato plants through enhancing growth and activity of antioxidant enzymes

Ali,

Ohri

2023

South African Journal of Botany

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Arabidopsis thaliana AtHRS1 gene is involved in the response to Heterodera schachtii infection and its overexpression hampers development of syncytia and involves a jasmonic acid-dependent mechanism

Cited by 5 publications

References 29 publications

The role of AtPP2-A3 and AtPP2-A8 genes encoding Nictaba-related lectin domains in the defense response of Arabidopsis thaliana to Heterodera schachtii

The role of AtPP2-A3 and AtPP2-A8 genes encoding Nictaba-related lectin domains in the defense response of Arabidopsis thaliana to Heterodera schachtii

Mutations in Selected ABA-Related Genes Reduce Level of Arabidopsis thaliana Susceptibility to the Beet Cyst Nematode Heterodera schachtii

Deciphering the synergistic effect of Jasmonic acid and Spermine in mitigating root-knot nematode stress in tomato plants through enhancing growth and activity of antioxidant enzymes

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