Arabidopsis SFAR4 is a novel GDSL-type esterase involved in fatty acid degradation and glucose tolerance

Huang, Li‐Min; Lai, Chia-Ping; Chen, Long-Fang O.; Chan, Ming‐Tsair; Shaw, Jei‐Fu

doi:10.1186/s40529-015-0114-6

Cited by 62 publications

(58 citation statements)

References 45 publications

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“…Major advances have been reported that GDSL lipases/esterases are involved in various biological functions, such as development of seeds [5][6][7][8][9], deposition of epicuticular wax [10], biosynthesis of cutin [11,12], hydration of pollen [13] and regulation of plant cell wall components [14]. Moreover, GDSL lipases are also involved in responses to various abiotic stresses, such as salt [15], drought [16], and freezing [17], as well as to biotic stresses, such as plant immune responses and pathogen defense [18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and analysis of GDSL-type esterases/lipases in watermelon (Citrullus lanatus)

Ren¹,

Yang²,

Xu³

et al. 2019

Preprint

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Background The GDSL esterase and lipase families play important roles in abiotic stress, pathogen defense, seed development and lipid metabolism. Identifying the lipase activity of a putative GDSL lipase is necessary to determine its function. Systematic analysis of the GDSL gene family is still lacking in Citrullus lanatus. Results In this study, we identified 65 watermelon GDSL-type esterase/lipase genes and divided these genes into 6 clades based on phylogeny. The phylogenetic relationship of watermelon GDSL genes compared with Arabidopsis thaliana GDSL esterases/lipases was also determined, and these genes were divided into four groups related to morphological development, abiotic stress response, pathogen defense, and secondary metabolism. The chromosomal location of these genes revealed that they are distributed unevenly across all 11 watermelon chromosomes. Analysis of duplication events suggested that segmental duplication and tandem duplication were the major driving forces of GDSL family evolution. Synteny analysis indicated that GDSLs in watermelon were highly homologous to those in Arabidopsis thaliana, melon and cucumber. Transcriptome analyses showed the tissue-specific and common expression of the GDSL genes in leaf and root tissues and identified nitrogen-related genes under low nitrogen (N) stress compared with optimal N conditions. Conclusions Our results provide a basis for selecting candidate watermelon GDSL genes for further studies to determine the biological functions of the GDSL genes in watermelon.

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Section: Introductionmentioning

confidence: 99%

Genome-wide identification and analysis of GDSL-type esterases/lipases in watermelon (Citrullus lanatus)

Ren¹,

Yang²,

Xu³

et al. 2019

Preprint

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“…It is believed that the substratebinding pocket of GDSL-hydrolases is flexible, allowing a broader substrate specificity (Akoh et al, 2004). The genome of Arabidopsis contains > 100 putative GDSL lipases, and some of them have been studied in some detail (Kelly & Feussner, 2016) and show, for example, esterase activity against artificial substrates (Huang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the enzymatic activity and physiological function of the lipid droplet‐associated triacylglycerol lipase AtOBL1

Müller¹,

Ischebeck²

2017

New Phytologist

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Similar to seeds, pollen tubes contain lipid droplets that store triacylglycerol (TAG), but the fate of this TAG as well as the enzymes involved in its breakdown are unknown. Therefore, two potential TAG lipases from tobacco and Arabidopsis, NtOBL1 (Oil body lipase 1) and AtOBL1, were investigated, especially with respect to their importance for pollen tube growth. We expressed NtOBL1 and AtOBL1 as fluorescent fusion proteins to study their localization by confocal microscopy. Furthermore, we overexpressed AtOBL1 in Nicotiana benthamiana leaves to characterize it enzymatically. The obl1 mutant was studied in respect to its pollen tube growth in vivo and its seed germination. Both NtOBL1 and AtOBL1 localized to lipid droplets. AtOBL1 was abundant in pollen tubes and seedlings, and acted as a lipase on TAG, diacylglycerol and 1-monoacylglycerol at a pH optimum of 5.5. The obl1 mutant was hampered in pollen tube growth, whereas seedling establishment was not affected under optimal conditions, even though AtOBL1 accounted for a major lipase activity in seeds. TAG could be a direct precursor for the synthesis of membrane lipids in pollen tubes and proteins of the OBL family involved in the flux of acyl groups.

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“…SFAR4 , a GDSL‐type esterase. SFAR4 plays an important role in fatty acid degradation (Huang et al ), and its primary metabolites function in plant disease resistance (Bolton ). Pectin is a major constituent of the primary cell wall, and pectin hydrolysis will cause cells to separate from each other (Fry , Harholt et al ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling reveals mechanisms of sexually dimorphic responses of Populus cathayana to potassium deficiency

Han

Song

Yang

et al. 2017

Physiologia Plantarum

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Potassium (K) deficiency causes a series of physiological and metabolic disorders in plants, and dioecious species exhibit different responses based on sex. Our previous morphological and physiological observations indicated that Populus cathayana males were more tolerant to K deficiency than females. To continue this work, comparative transcriptome analyses were carried out to investigate sexually differentially expressed genes (DEGs) in this study. The results indicate that 10 weeks of K deficiency result in 111 and 181 DEGs in males and females, respectively. These DEGs are mainly involved in photosynthesis, cell wall biosynthesis, secondary metabolism, transport, stress responses, gene expression regulation and protein synthesis and degradation. Comparing between sexes, P. cathayana females showed more changes in response to K deficiency than males with regard to photosynthesis, gene expression regulation and posttranslational modification but fewer changes in secondary metabolism, stress responses and redox homeostasis. These results provide evidence that P. cathayana females are more susceptible to K deficiency than males. Therefore, there are sex-related molecular strategies in response to K deficiency between sexes.

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Arabidopsis SFAR4 is a novel GDSL-type esterase involved in fatty acid degradation and glucose tolerance

Cited by 62 publications

References 45 publications

Genome-wide identification and analysis of GDSL-type esterases/lipases in watermelon (Citrullus lanatus)

Genome-wide identification and analysis of GDSL-type esterases/lipases in watermelon (Citrullus lanatus)

Characterization of the enzymatic activity and physiological function of the lipid droplet‐associated triacylglycerol lipase AtOBL1

Transcriptional profiling reveals mechanisms of sexually dimorphic responses of Populus cathayana to potassium deficiency

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