Arabidopsis plastidial folylpolyglutamate synthetase is required for nitrogen metabolism under nitrate-limited condition in darkness

Meng, Huaqing; Xu, Bosi; Zhang, Chunyi; Ling, Jiang

doi:10.1016/j.bbrc.2016.11.054

Cited by 7 publications

(9 citation statements)

References 27 publications

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“…The disrupted folate metabolism in the atdfb‐3 seedlings interfered with the synthesis or metabolism of sugars, polyols, and fatty acids. Under the nitrate‐limited condition, the total folate and 5‐M‐THF contents were lower in the mutant than in the wild‐type control, which is in contrast to the 5‐F‐THF content (Meng et al, 2014; Meng et al, 2017). Moreover, the mutant accumulated various metabolites, including fructose, galactose, glucopyranoside, mannose, campesterol, stigmasterol, and fatty acids (e.g., 18:3, 20:1, and 24:0).…”

Section: Discussionmentioning

confidence: 87%

“…A mutation to AtDFC , which encodes a mitochondrial folylpolyglutamate synthetase (FPGS), leads to altered nitrogen metabolism and pronounced phenotypes in response to low‐nitrogen stress (Jiang et al, 2013). A mutation to AtDFB , which encodes a plastidial FPGS isoform, modulates the glutamylation status of some folates and broadly alters metabolism under both dark and light conditions (Hayashi et al, 2017; Meng et al, 2014; Meng et al, 2017; Srivastava et al, 2011). Folate polyglutamylation is also required for global DNA methylation and histone H3 lysine 9 dimethylation through its function related to one‐carbon metabolism (Zhou et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3)

Meng

Liu

et al. 2022

Plant Direct

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The metabolic networks underlying skotomorphogenesis in seedlings remain relatively unknown. On the basis of our previous study on the folate metabolism in seedlings grown in darkness, the plastidial folylpolyglutamate synthetase gene (AtDFB) T‐DNA insertion Arabidopsis thaliana mutant (atdfb‐3) was examined. Under the nitrate‐sufficient condition, the mutant exhibited deficient folate metabolism and hypocotyl elongation, which affected skotomorphogenesis. Further analyses revealed changes to multiple intermediate metabolites related to carbon and nitrogen metabolism in the etiolated atdfb‐3 seedlings. Specifically, the sugar, polyol, and fatty acid contents decreased in the atdfb‐3 mutant under the nitrate‐sufficient condition, whereas the abundance of various organic acids and amino acids increased. In response to nitrate‐limited stress, multiple metabolites, including sugars, polyols, fatty acids, organic acids, and amino acids, accumulated more in the mutant than in the wild‐type control. The differences in the contents of multiple metabolites between the atdfb‐3 and wild‐type seedlings decreased following the addition of exogenous 5‐F‐THF under both nitrogen conditions. Additionally, the mutant accumulated high levels of one‐carbon metabolites, such as Cys, S‐adenosylmethionine, and S‐adenosylhomocysteine, under both nitrogen conditions. Thus, our data demonstrated that the perturbed folate metabolism in the atdfb‐3 seedlings, which was caused by the loss‐of‐function mutation to AtDFB, probably altered carbon and nitrogen metabolism, thereby modulating skotomorphogenesis. Furthermore, the study findings provide new evidence of the links among folate metabolism, metabolic networks, and skotomorphogenesis.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3)

Meng

Liu

et al. 2022

Plant Direct

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“…The FPGS gene-encoding folylpolyglutamate synthetase responds to the formation of folate polyglutamylation as a key regulatory point of folate metabolism. atdfb/atdfc (encoding FPGS) mutant seedlings had deficiencies in aa, nucleotides, and starch metabolism. ,− However, the associations between folate and other amino acid metabolism pathways are still unclear. Here, the more abundant metabolites of the two SiFBP TLs and the WT were investigated via untargeted metabolome analysis.…”

Section: Discussionmentioning

confidence: 99%

Heterologous Expression of SiFBP, a Folate-Binding Protein from Foxtail Millet, Confers Increased Folate Content and Altered Amino Acid Profiles with Nutritional Potential to Arabidopsis

Hou

Zhang

Zhao

et al. 2022

J. Agric. Food Chem.

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The mechanism underlying folate degradation in foxtail millet grains remains unclear. Here, we identified SiFBP (Setaria italica folate-binding protein) from foxtail millet. A phylogenetic tree revealed that FBPs have close genetic relationships among cereal crop species. Docking analysis and heterologous expression of SiFBP in yeast showed that it could bind folic acid (FA). The SiFBP localized to the plasma membrane in tobacco mesophyll cells by transient expression. In Arabidopsis, it was expressed specifically in the roots and germinating seeds. Overexpressing SiFBP in yeast and Arabidopsis significantly increased folate contents. Untargeted metabolome analysis revealed differentially accumulated metabolites between the transgenic lines (TLs) and wild type (WT); these metabolites were mainly enriched in the amino acid metabolism pathway. The relative contents of lysine and leucine, threonine, and L-methionine were significantly higher in the TLs than in WT. Genes related to the folate and lysine synthesis pathways were upregulated in the TLs. Thus, SiFBP can be used for biofortification of folate and important amino acids in crops via genetic engineering.

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“…The accumulation of amino acids in wheat and alfalfa were reported to enhance the tolerance under drought conditions and NaCl stress, respectively (Bowne et al , Bertrand et al ). In a study of nitrogen metabolism under nitrate‐limited and dark conditions, Glu, Gln, Asp, Asn and Pro coincidently restored nitrogen content to wild‐type levels in a T‐DNA insertion mutant of Arabidopsis plastidial folylpolyglutamate synthetase gene (Meng et al ). The high Pro content in transgenic tobacco was also suggested to be attributable to excess Gln production (Wang et al , ).…”

Section: Discussionmentioning

confidence: 99%

A metabolomic strategy revealed the role of JA and SA balance in Clematis terniflora DC. Response to UVB radiation and dark

Yang

Chen

Wang

et al. 2019

Physiologia Plantarum

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Clematis terniflora DC. is a valuable resource with potential high pharmaceutical value. Proteomic, transcriptomic and metabolomic analyses of C. terniflora that has been exposed to high levels of UVB irradiation and dark conditions (HUVB + D) have revealed the mechanisms underlying its medicinal potential. However, the signal transduction pathways and the mechanisms of regulation for the accumulation of secondary metabolites remain unclear. In this study, we show that the jasmonic acid (JA) and salicylic acid (SA) signals were activated in C. terniflora in response to HUVB + D. Metabolomic analysis demonstrated that the perturbation in JA and SA balance led to additional reallocation of carbon and nitrogen resources. Evaluating the fold change ratios of differentially changed metabolites proved that JA signal enhanced the transformation of nitrogen to carbon through the 4‐aminobutyric acid (GABA) shunt pathway, which increased the carbon reserve to be utilized in the production of secondary metabolites. However, SA signal induced the synthesis of proline, while avoiding the accumulation of secondary metabolites. Over all, the results indicate that the co‐increase of JA and SA reconstructed the dynamic stability of transformation from nitrogen to carbon, which effectively enhanced the oxidative defense to HUVB + D in C. terniflora by increasing the secondary metabolites.

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Arabidopsis plastidial folylpolyglutamate synthetase is required for nitrogen metabolism under nitrate-limited condition in darkness

Cited by 7 publications

References 27 publications

Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3)

Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3)

Heterologous Expression of SiFBP, a Folate-Binding Protein from Foxtail Millet, Confers Increased Folate Content and Altered Amino Acid Profiles with Nutritional Potential to Arabidopsis

A metabolomic strategy revealed the role of JA and SA balance in Clematis terniflora DC. Response to UVB radiation and dark

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