Arabidopsis <i>HIPP27</i> is a host susceptibility gene for the beet cyst nematode <i>Heterodera schachtii</i>

Radakovic, Zoran S.; Anjam, Muhammad Shahzad; Escobar, Elizabeth; Chopra, Divykriti; Cabrera, Javier; Silva, Ana Cláudia; Escobar, Carolina; Sobczak, Mirosław; Grundler, Florian M. W.; Siddique, Shahid

doi:10.1101/208132

Cited by 12 publications

(16 citation statements)

References 32 publications

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“…Some previous studies have mentioned degradation of the endodermis and the presence of periderm‐like tissue around syncytia (Golinowski et al ., ; Sobczak et al ., ; Radakovic et al ., ; Różańska et al ., ). To analyze this anatomical problem in more detail, we conducted serial sectioning of samples with nematode infection sites at 3, 5 and 10 dai (Figures and ).…”

Section: Resultsmentioning

confidence: 99%

Root endodermal barrier system contributes to defence against plant‐parasitic cyst and root‐knot nematodes

et al. 2019

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Plant-parasitic nematodes (PPNs) cause tremendous yield losses worldwide in almost all economically important crops. The agriculturally most important PPNs belong to a small group of root-infecting sedentary endoparasites that includes cyst and root-knot nematodes. Both cyst and root-knot nematodes induce specialized long-term feeding structures in root vasculature from which they obtain their nutrients. A specialized cell layer in roots called the endodermis, which has cell walls reinforced with suberin deposits and a lignin-based Casparian strip (CS), protects the vascular cylinder against abiotic and biotic threats. To date, the role of the endodermis, and especially of suberin and the CS, during plant-nematode interactions was largely unknown. Here, we analyzed the role of suberin and CS during interaction between Arabidopsis plants and two sedentary root-parasitic nematode species, the cyst nematode Heterodera schachtii and the root-knot nematode Meloidogyne incognita. We found that nematode infection damages the endodermis leading to the activation of suberin biosynthesis genes at nematode infection sites. Although feeding sites induced by both cyst and root-knot nematodes are surrounded by endodermis during early stages of infection, the endodermis is degraded during later stages of feeding site development, indicating periderm formation or ectopic suberization of adjacent tissue. Chemical suberin analysis showed a characteristic suberin composition resembling peridermal suberin in nematode-infected tissue. Notably, infection assays using Arabidopsis lines with CS defects and impaired compensatory suberization, revealed that the CS and suberization impact nematode infectivity and feeding site size. Taken together, our work establishes the role of the endodermal barrier system in defence against a soil-borne pathogen.

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Section: Resultsmentioning

confidence: 99%

Root endodermal barrier system contributes to defence against plant‐parasitic cyst and root‐knot nematodes

et al. 2019

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“…Studies of the interactomes of effectors from different phytopathogens have identified common host targets, proposed to be immunity-related “hubs,” which are targeted by diverse pathogens to achieve infection ( 48 ). The movement protein of potato mop top virus interacts with NbHIPP26 to promote long-distance viral movement ( 49 ), and AtHIPP27 is reported to be a susceptibility factor for infection by the beet cyst nematode Heterodera schachtii ( 50 ). There may be more as-yet unidentified effectors, produced by diverse pathogens, which target HIPPs or HPPs to promote infection.…”

Section: Discussionmentioning

confidence: 99%

Multiple variants of the fungal effector AVR-Pik bind the HMA domain of the rice protein OsHIPP19, providing a foundation to engineer plant defense

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Franceschetti

Maqbool

et al. 2021

Journal of Biological Chemistry

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“…Studies of the interactomes of effectors from different phytopathogens have identified common host targets, proposed to be immunity-related "hubs" which are targeted by diverse pathogens to achieve infection (48). The movement protein of potato mop top virus interacts with NbHIPP26 to promote long-distance viral movement (49), and AtHIPP27 is reported to be a susceptibility factor for infection by the beet cyst nematode Heterodera schachtii (50). There may be more as-yet unidentified effectors, produced by diverse pathogens, which target HIPPs or HPPs to promote infection.…”

Section: Hipps and Hpps May Be Plant Immunity Hubs And Are Targeted mentioning

confidence: 99%

Multiple variants of the blast fungus effector AVR-Pik bind the HMA domain of the rice protein OsHIPP19 with high affinity

Maidment

Franceschetti

Maqbool

et al. 2020

Preprint

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Microbial plant pathogens secrete effector proteins which manipulate the host to promote infection. Effectors can be recognised by plant intracellular nucleotide-binding leucine-rich repeat (NLR) receptors, initiating an immune response. The AVR-Pik effector from the rice blast fungus Magnaporthe oryzae is recognised by a pair of rice NLR receptors, Pik-1 and Pik-2. Pik-1 contains a non-canonical integrated heavy metal-associated (HMA) domain, which directly binds AVR-Pik to activate plant defences. Non-canonical integrated domains are widespread in plant NLRs and are thought to resemble the host target of the recognised effector. AVR-Pik interacts with specific rice HMA domain-containing proteins, namely heavy metal-associated isoprenylated plant proteins (HIPPs) and heavy metal-associated plant proteins (HPPs). Here, we define the biochemical and structural basis of the interaction between AVR-Pik and OsHIPP19, and compare the interaction with the HMA domain of Pik-1. Using analytical gel filtration and surface plasmon resonance, we show that multiple AVR-Pik variants, including the stealthy variants AVR-PikC and AVR-PikF which do not interact with any characterised Pik-1 alleles, bind to OsHIPP19 with nanomolar affinity. The crystal structure of OsHIPP19 in complex with AVR-PikF reveals differences at the interface that underpin high-affinity binding of OsHIPP19-HMA to a wider set of AVR-Pik variants than achieved by the integrated HMA domain of Pik-1. Our results provide a foundation for engineering the HMA domain of Pik-1 to extend binding to currently unrecognised AVR-Pik variants and expand disease resistance in rice to divergent pathogen strains.

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Arabidopsis HIPP27 is a host susceptibility gene for the beet cyst nematode Heterodera schachtii

Cited by 12 publications

References 32 publications

Root endodermal barrier system contributes to defence against plant‐parasitic cyst and root‐knot nematodes

Root endodermal barrier system contributes to defence against plant‐parasitic cyst and root‐knot nematodes

Multiple variants of the fungal effector AVR-Pik bind the HMA domain of the rice protein OsHIPP19, providing a foundation to engineer plant defense

Multiple variants of the blast fungus effector AVR-Pik bind the HMA domain of the rice protein OsHIPP19 with high affinity

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