Arabidopsis florigen FT binds to diurnally oscillating phospholipids that accelerate flowering

Nakamura, Yuki; Andrés, Fernando; Kanehara, Kazue; Liu, Yu-chi; Dörmann, Peter; Coupland, George

doi:10.1038/ncomms4553

Cited by 147 publications

(156 citation statements)

References 32 publications

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…Polar lipid profiling showed that, similar to findings on the previously characterized tgd1-1 mutant (Xu et al, 2005), tgd5 mutants displayed substantially reduced levels of galactolipids with concomitant increases in PC ( Figure 3B). It has recently been shown that increased PC promotes flowering via interaction with the Arabidopsis florigen FT (Nakamura et al, 2014). Therefore, it is possible that the early flowering phenotype of tgd mutants is due to increases in PC levels.…”

Section: The Tgd5-1 Mutation Defines Arabidopsis Gene At1g27695mentioning

confidence: 99%

Arabidopsis TRIGALACTOSYLDIACYLGLYCEROL5 Interacts with TGD1, TGD2, and TGD4 to Facilitate Lipid Transfer from the Endoplasmic Reticulum to Plastids

et al. 2015

View full text Add to dashboard Cite

The biogenesis of photosynthetic membranes in the plastids of higher plants requires an extensive supply of lipid precursors from the endoplasmic reticulum (ER). Four TRIGALACTOSYLDIACYLGLYCEROL (TGD) proteins (TGD1,2,3,4) have thus far been implicated in this lipid transfer process. While TGD1, TGD2, and TGD3 constitute an ATP binding cassette transporter complex residing in the plastid inner envelope, TGD4 is a transmembrane lipid transfer protein present in the outer envelope. These observations raise questions regarding how lipids transit across the aqueous intermembrane space. Here, we describe the isolation and characterization of a novel Arabidopsis thaliana gene, TGD5. Disruption of TGD5 results in similar phenotypic effects as previously described in tgd1,2,3,4 mutants, including deficiency of ER-derived thylakoid lipids, accumulation of oligogalactolipids, and triacylglycerol. Genetic analysis indicates that TGD4 is epistatic to TGD5 in ER-to-plastid lipid trafficking, whereas double mutants of a null tgd5 allele with tgd1-1 or tgd2-1 show a synergistic embryo-lethal phenotype. TGD5 encodes a small glycine-rich protein that is localized in the envelope membranes of chloroplasts. Coimmunoprecipitation assays show that TGD5 physically interacts with TGD1, TGD2, TGD3, and TGD4. Collectively, these results suggest that TGD5 facilitates lipid transfer from the outer to the inner plastid envelope by bridging TGD4 with the TGD1,2,3 transporter complex.

show abstract

Section: The Tgd5-1 Mutation Defines Arabidopsis Gene At1g27695mentioning

confidence: 99%

Arabidopsis TRIGALACTOSYLDIACYLGLYCEROL5 Interacts with TGD1, TGD2, and TGD4 to Facilitate Lipid Transfer from the Endoplasmic Reticulum to Plastids

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The nmt1 nmt3 mutant is a powerful tool for analyzing the physiological functions of including those that appear to preferentially bind to FT (Nakamura et al, 2014 navigation towards the ovule and egg cell (Higashiyama and Takeuchi, 2015). This supports 665 a need for future investigation under a range of conditions as a temperature-conditional male 666 sterility has been reported in an NMT1 silencing line (Mou et al, 2002) and should include 667 examining the possibility that, in the homozygous nmt1 nmt3 double mutant, female 668 sporophytic defects may influence the male gametophyte, such as observed in some female 669 gametophytic mutants (Huck et al, 2003), especially given the strong and specific expression 670 of NMT3 in the synergid area during late ovule development (Fig.…”

mentioning

confidence: 99%

NMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction

et al. 2018

View full text Add to dashboard Cite

54Phosphatidylcholine (PC) is a major membrane phospholipid and a precursor for most 55 signalling molecules. Understanding its synthesis is important for improving plant growth, 56 nutritional value and resistance to stress. PC synthesis is complex, involving several 57 interconnected pathways, one of which proceeds from serine-derived phosphoethanolamine 58(PEA) to form phosphocholine (PCho) through three sequential phospho-base methylations 59 catalysed by phosphoethanolamine N-methyltransferases (PEAMTs). The contribution of 60 this pathway to the production of PC and plant growth has been a matter of some debate.

show abstract

“…Future research will focus on verifying how these alterations can preserve the lipid composition in membranes while ensuring their effective recycling in Pi-starved conditions. Ultimately, this will help to identify key elements of membrane lipid control, an important topic considering the emerging regulatory role of PtdCho in the control of plant responses to the environment (Nakamura et al, 2014). …”

mentioning

confidence: 99%

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

et al. 2018

View full text Add to dashboard Cite

Phosphate starvation-mediated induction of the HAD-type phosphatases PPsPase1 (AT1G73010) and PECP1 (AT1G17710) has been reported in Arabidopsis (Arabidopsis thaliana). However, little is known about their in vivo function or impact on plant responses to nutrient deficiency. The preferences of PPsPase1 and PECP1 for different substrates have been studied in vitro but require confirmation in planta. Here, we examined the in vivo function of both enzymes using a reverse genetics approach. We demonstrated that PPsPase1 and PECP1 affect plant phosphocholine and phosphoethanolamine content, but not the pyrophosphate-related phenotypes. These observations suggest that the enzymes play a similar role in planta related to the recycling of polar heads from membrane lipids that is triggered during phosphate starvation. Altering the expression of the genes encoding these enzymes had no effect on lipid composition, possibly due to compensation by other lipid recycling pathways triggered during phosphate starvation. Furthermore, our results indicated that PPsPase1 and PECP1 do not influence phosphate homeostasis, since the inactivation of these genes had no effect on phosphate content or on the induction of molecular markers related to phosphate starvation. A combination of transcriptomics and imaging analyses revealed that PPsPase1 and PECP1 display a highly dynamic expression pattern that closely mirrors the phosphate status. This temporal dynamism, combined with the wide range of induction levels, broad expression, and lack of a direct effect on Pi content and regulation, makes PPsPase1 and PECP1 useful molecular markers of the phosphate starvation response.Plant growth is highly sensitive to a lack of phosphate (Pi); hence, the application of Pi fertilizers has become standard practice for high-throughput crop production (Cordell et al., 2009). Most phosphorus in the soil is not available to plants, as it is combined with other minerals or parts of organic compounds (Bieleski, 1973;Raghothama and Karthikeyan, 2005). Only a small fraction of soluble Pi (usually present at a concentration of ,10 mM in the soil) can be taken up by plants.Although growth is not optimal under limiting conditions, plants can withstand changing Pi concentrations within heterogeneous soils or in the external nutrient supply that can lead to reprogramming of their metabolism and architecture (Hammond et al., 2003;Péret et al., 2011;Plaxton and Tran, 2011). Root architecture can be modified to facilitate Pi uptake by favoring the development of lateral roots (at the expense of primary root elongation in many plants including Arabidopsis), increasing the density and length of root hairs, and limiting the development of aerial parts (López-Bucio et al., 2002;Svistoonoff et al., 2007;Gruber et al., 2013). Pi uptake mechanisms are enhanced at the root/soil interface, particularly through the stimulation of Pi transport activity (Mudge et al., 2002;Shin et al., 2004;Nussaume et al., 2011; Ayadi et al., 2015). In parallel, mobilization of Pi sources fr...

show abstract

Arabidopsis florigen FT binds to diurnally oscillating phospholipids that accelerate flowering

Cited by 147 publications

References 32 publications

Arabidopsis TRIGALACTOSYLDIACYLGLYCEROL5 Interacts with TGD1, TGD2, and TGD4 to Facilitate Lipid Transfer from the Endoplasmic Reticulum to Plastids

Arabidopsis TRIGALACTOSYLDIACYLGLYCEROL5 Interacts with TGD1, TGD2, and TGD4 to Facilitate Lipid Transfer from the Endoplasmic Reticulum to Plastids

NMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction

The Phosphate Fast-Responsive Genes PECP1 and PPsPase1 Affect Phosphocholine and Phosphoethanolamine Content

Contact Info

Product

Resources

About