Arabidopsis DREB2C modulates ABA biosynthesis during germination

Je, Jihyun; Chen, Huan; Song, Chieun; Lim, Chae Oh

doi:10.1016/j.bbrc.2014.08.052

Cited by 34 publications

(30 citation statements)

References 22 publications

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“…Previous studies demonstrated that the NCED gene can be induced by exogenous ABA2533. DREB2C, ATHB and ATAF1 TFs are known to be activators of NCED transcription, but the negative regulation of NCED is still unclear123435. However, ABA is likely to be involved, probably as part of a negative feedback mechanism, because such regulation by the product (s) of a biosynthetic pathway is ubiquitous in plants.…”

Section: Discussionmentioning

confidence: 99%

Negative feedback regulation of ABA biosynthesis in peanut (Arachis hypogaea): a transcription factor complex inhibits AhNCED1 expression during water stress

Liu

et al. 2016

Sci Rep

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Abscisic acid (ABA), a key plant stress-signaling hormone, is produced in response to drought and counteracts the effects of this stress. The accumulation of ABA is controlled by the enzyme 9-cis-epoxycarotenoid dioxygenase (NCED). In Arabidopsis, NCED3 is regulated by a positive feedback mechanism by ABA. In this study in peanut (Arachis hypogaea), we demonstrate that ABA biosynthesis is also controlled by negative feedback regulation, mediated by the inhibitory effect on AhNCED1 transcription of a protein complex between transcription factors AhNAC2 and AhAREB1. AhNCED1 was significantly down-regulated after PEG treatment for 10 h, at which time ABA content reached a peak. A ChIP-qPCR assay confirmed AhAREB1 and AhNAC2 binding to the AhNCED1 promoter in response to ABA. Moreover, the interaction between AhAREB1 and AhNAC2, and a transient expression assay showed that the protein complex could negatively regulate the expression of AhNCED1. The results also demonstrated that AhAREB1 was the key factor in AhNCED1 feedback regulation, while AhNAC2 played a subsidiary role. ABA reduced the rate of AhAREB1 degradation and enhanced both the synthesis and degradation rate of the AhNAC2 protein. In summary, the AhAREB1/AhNAC2 protein complex functions as a negative feedback regulator of drought-induced ABA biosynthesis in peanut.

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Section: Discussionmentioning

confidence: 99%

Negative feedback regulation of ABA biosynthesis in peanut (Arachis hypogaea): a transcription factor complex inhibits AhNCED1 expression during water stress

Liu

et al. 2016

Sci Rep

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“…Other than SlZFP2, several transcription factors have been shown to directly target the ABA biosynthetic genes NCEDs and AAO3 in rice and Arabidopsis, respectively (Yaish et al, 2010;Jiang et al, 2012;Jensen et al, 2013;Je et al, 2014;Yang et al, 2014). Recently, a tomato C1-2i member of the C 2 H 2 -type zinc finger proteins SlZF2 also was shown to regulate ABA biosynthesis because of increased ABA production in its overexpression lines (Hichri et al, 2014).…”

Section: Direct Target Genes Of Slzfp2mentioning

confidence: 99%

“…The rice (Oryza sativa) APETALA2 (AP2)-like gene OsAP2-39 targets OsNECD1, and the Arabidopsis NECD3 is the direct target of the WRKY transcription factor ACQUIRED DROUGHT TOLERANCE and the NAC (for no apical meristem [NAM], Arabidopsis transcription activation factor [ATAF], and cup-shaped cotyledon [CUC]) family member ATAF1 (Yaish et al, 2010;Jiang et al, 2012;Jensen et al, 2013). Recently, two more transcription factors, the AP2/ETHYLENE RESPONSE FACTOR (ERF) family member dehydration-responsive element-binding protein 2C and the NAC-like gene ACTIVATED BY AP3/PI, have been shown to directly activate NCED9 and AAO3 expression in Arabidopsis during seed germination and leaf senescence (Je et al, 2014;Yang et al, 2014).…”

Section: Supersensitive To Abscisic Acid and Drought1mentioning

confidence: 99%

The Zinc Finger Transcription Factor SlZFP2 Negatively Regulates Abscisic Acid Biosynthesis and Fruit Ripening in Tomato

Weng

Zhao

et al. 2015

Plant Physiol.

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Abscisic acid (ABA) regulates plant development and adaptation to environmental conditions. Although the ABA biosynthesis pathway in plants has been thoroughly elucidated, how ABA biosynthetic genes are regulated at the molecular level during plant development is less well understood. Here, we show that the tomato (Solanum lycopersicum) zinc finger transcription factor SlZFP2 is involved in the regulation of ABA biosynthesis during fruit development. Overexpression of SlZFP2 resulted in multiple phenotypic changes, including more branches, early flowering, delayed fruit ripening, lighter seeds, and faster seed germination, whereas downregulation of its expression caused problematic fruit set, accelerated ripening, and inhibited seed germination. SlZFP2 represses ABA biosynthesis during fruit development through direct suppression of the ABA biosynthetic genes NOTABILIS, SITIENS, and FLACCA and the aldehyde oxidase SlAO1. We also show that SlZFP2 regulates fruit ripening through transcriptional suppression of the ripening regulator COLORLESS NON-RIPENING. Using bacterial one-hybrid screening and a selected amplification and binding assay, we identified the (A/T)(G/C)TT motif as the core binding sequence of SlZFP2. Furthermore, by RNA sequencing profiling, we found that 193 genes containing the SlZFP2-binding motifs in their promoters were differentially expressed in 2 d post anthesis fruits between the SlZFP2 RNA interference line and its nontransgenic sibling. We propose that SlZFP2 functions as a repressor to fine-tune ABA biosynthesis during fruit development and provides a potentially valuable tool for dissecting the role of ABA in fruit ripening.

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“…In addition, CHOTTO1, a putative double AP2 repeat transcription factor in Arabidopsis , represses GA biosynthesis during seed germination (Yano et al ., ). Furthermore, another ATF from Arabidopsis , DREB2C (Dehydration‐Responsive Element Binding factor 2C), also acts as a positive regulator of ABA biosynthesis in germinating seeds by activating expression of the ABA‐biosynthesis gene NCED9 (Je et al ., ). However, the relationship between DDF1/CHOTTO1 and the ABA biosynthesis pathway, and between DREB2C and the GA metabolism pathway, is still elusive (Magome et al ., ; Yano et al ., ; Je et al ., ).…”

Section: Several Other Atfs Are Involved In the Regulation Of Balancementioning

confidence: 97%

APETALA 2‐domain‐containing transcription factors: focusing on abscisic acid and gibberellins antagonism

2017

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Summary The phytohormones abscisic acid (ABA) and gibberellin (GA) antagonistically mediate diverse plant developmental processes including seed dormancy and germination, root development, and flowering time control, and thus the optimal balance between ABA and GA is essential for plant growth and development. Although more than a half and one century have passed since the initial discoveries of ABA and GA, respectively, the precise mechanisms underlying ABA–GA antagonism still need further investigation. Emerging evidence indicates that two APETALA 2 (AP2)‐domain‐containing transcription factors (ATFs), ABI4 in Arabidopsis and OsAP2‐39 in rice, play key roles in ABA and GA antagonism. These two transcription factors precisely regulate the transcription pattern of ABA and GA biosynthesis or inactivation genes, mediating ABA and GA levels. In this Viewpoint article, we try to shed light on the effects of ATFs on ABA–GA antagonism, and summarize the overlapping but distinct biological functions of these ATFs in the antagonism between ABA and GA. Finally, we strongly propose that further research is needed into the detailed roles of additional numerous ATFs in ABA and GA crosstalk, which will improve our understanding of the antagonism between these two phytohormones.

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Arabidopsis DREB2C modulates ABA biosynthesis during germination

Cited by 34 publications

References 22 publications

Negative feedback regulation of ABA biosynthesis in peanut (Arachis hypogaea): a transcription factor complex inhibits AhNCED1 expression during water stress

Negative feedback regulation of ABA biosynthesis in peanut (Arachis hypogaea): a transcription factor complex inhibits AhNCED1 expression during water stress

The Zinc Finger Transcription Factor SlZFP2 Negatively Regulates Abscisic Acid Biosynthesis and Fruit Ripening in Tomato

APETALA 2‐domain‐containing transcription factors: focusing on abscisic acid and gibberellins antagonism

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