Arabidopsis CHROMATIN REMODELING 19 acts as a transcriptional repressor and contributes to plant pathogen resistance

Kang, Huijia; Liu, Yuhao; Fan, Tianyi; Ma, Jing; Wu, Di; Heitz, Thierry; Shen, Wenhui; Zhu, Yan

doi:10.1093/plcell/koab318

Cited by 13 publications

(7 citation statements)

References 77 publications

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“…We next plotted the ChIP-seq signals of SYD, BRM, SPT6LΔtSH2, and SPT6L at SYD-occupied genes and also found the genome-wide concurrent signals of SYD, BRM and SPT6LΔtSH2 at TSS (Figure 1B ). To examine the specificity of co-binding between SWI2/SNF2 and SPT6LΔtSH2, we also plotted four other chromatin remodelers on the same genomic regions and found that except BAF60 (SWI/SNF family) ( 55 ), other three chromatin remodelers PHOTOPERIOD INDEPENDENT EARLY FLOWERING 1 (PIE1, SWI2/SNF2-related 1 family) ( 56 ), CHROMATIN REMODELING 11 (CHR11, ISWI family) ( 56 ), and a putative SNF2-related chromatin remodeler CHR19 ( 57 ) were weakly or even not enriched at TSS (Figure 1B ). This result suggests that SPT6LΔtSH2 may prefer to interplay with SWI/SNF complex at TSS.…”

Section: Resultsmentioning

confidence: 99%

Transcription elongator SPT6L regulates the occupancies of the SWI2/SNF2 chromatin remodelers SYD/BRM and nucleosomes at transcription start sites in Arabidopsis

Ding

Liu

Cui

et al. 2022

Nucleic Acids Research

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Chromatin remodelers have been thought to be crucial in creating an accessible chromatin environment before transcription activation. However, it is still unclear how chromatin remodelers recognize and bind to the active regions. In this study, we found that chromatin remodelers SPLAYED (SYD) and BRAHMA (BRM) interact and co-occupy with Suppressor of Ty6-like (SPT6L), a core subunit of the transcription machinery, at thousands of the transcription start sites (TSS). The association of SYD and BRM to chromatin is dramatically reduced in spt6l and can be restored mainly by SPT6LΔtSH2, which binds to TSS in a RNA polymerase II (Pol II)-independent manner. Furthermore, SPT6L and SYD/BRM are involved in regulating the nucleosome and Pol II occupancy around TSS. The presence of SPT6L is sufficient to restore the association of the chromatin remodeler SYD to chromatin and maintain normal nucleosome occupancy. Our findings suggest that the two chromatin remodelers can form protein complexes with the core subunit of the transcription machinery and regulate nucleosome occupancy in the early transcription stage.

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Section: Resultsmentioning

confidence: 99%

Transcription elongator SPT6L regulates the occupancies of the SWI2/SNF2 chromatin remodelers SYD/BRM and nucleosomes at transcription start sites in Arabidopsis

Ding

Liu

Cui

et al. 2022

Nucleic Acids Research

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“…Previous studies have shown that OsCHR725 ( OsBRHIS1 ) plays a critical role in the SA-independent disease resistance ( Li et al, 2015 ), suggesting that the cis -acting elements in the promoter are coordinated with the gene expression and function. AtCHR19 acts as a transcriptional repressor and contributes to plant pathogen resistance ( Kang et al, 2022 ). OsCHR714 , the homologous gene of AtCHR19 , was significantly upregulated at the 12 hpi of M. oryzae and all treatment timelines of salt and drought stress.…”

Section: Discussionmentioning

confidence: 99%

Comparative Expression Profiling of Snf2 Family Genes During Reproductive Development and Stress Responses in Rice

Guo

Zhao

et al. 2022

Front. Plant Sci.

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Sucrose non-fermenting 2 (Snf2) protein family, as chromatin remodeling factors, is an enormous and the most diverse protein family, which contributes to biological processes of replication, transcription, and DNA repair using the energy of adenosine triphosphate (ATP) hydrolysis. The members of Snf2 family proteins have been well characterized in Arabidopsis, rice, and tomato. Although this family received significant attention, few genes were identified uniquely for their roles in mediating reproductive development and stress tolerance in rice. In the present study, we comprehensively analyzed the expression profiling of Snf2 genes during reproductive development and biotic/abiotic stresses. Our results showed that five proteins (OsCHR712/715/720/726/739) were mainly localized in the nucleus, while OsCHR715/739 were also slightly expressed in the cell membrane. There were abundant cis-acting elements in the putative promoter of Snf2 genes, including dehydration, MeJA, MYB binding site for drought, ABA-responsive, and stress-responsive element. Most of the genes were induced immediately after Magnaporthe oryzae infection at 12 h post-infection (hpi). About 55% of the total genes were upregulated under salt and drought stresses during the entire time, and 22–35% of the total genes were upregulated at 3 h. It was noteworthy that the seven genes (OsCHR705, OsCHR706, OsCHR710, OsCHR714, OsCHR721, OsCHR726, and OsCHR737) were upregulated, and one gene (OsCHR712) was downregulated under salt and drought stresses, respectively. The deficiency of OsCHR726 mutations displayed a hypersensitive phenotype under salt stress. These results will be significantly useful features for the validation of the rice Snf2 genes and facilitate understanding of the genetic engineering of crops with improved biotic and abiotic stresses.

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“…Reverse transcription (RT) was performed using Improm-II reverse transcriptase (Promega, Madison, WI, USA). RT-qPCR was performed in three biological replicates according to the standard instruction 48 . The primers used in this study were listed in Supplementary Data 8 .…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis γ-H2A.X-INTERACTING PROTEIN participates in DNA damage response and safeguards chromatin stability

Fan

Kang

et al. 2022

Nat Commun

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Upon the occurrence of DNA double strand breaks (DSB), the proximal histone variant H2A.X is phosphorylated as γ-H2A.X, a critical signal for consequent DSB signaling and repair pathways. Although γ-H2A.X-triggered DNA damage response (DDR) has been well-characterized in yeast and animals, the corresponding pathways in plant DDR are less well understood. Here, we show that an Arabidopsis protein γ-H2A.X-INTERACTING PROTEIN (XIP) can interact with γ-H2A.X. Its C-terminal dual-BRCT-like domain contributes to its specific interaction with γ-H2A.X. XIP-deficient seedlings display smaller meristems, inhibited growth, and higher sensitivity to DSB-inducing treatment. Loss-of-function in XIP causes transcriptome changes mimicking wild-type plants subject to replicative or genotoxic stresses. After genotoxic bleomycin treatment, more proteins with upregulated phosphorylation modifications, more DNA fragments and cell death were found in xip mutants. Moreover, XIP physically interacts with RAD51, the key recombinase in homologous recombination (HR), and somatic HR frequency is significantly reduced in xip mutants. Collectively, XIP participates in plant response to DSB and contributes to chromatin stability.

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Arabidopsis CHROMATIN REMODELING 19 acts as a transcriptional repressor and contributes to plant pathogen resistance

Cited by 13 publications

References 77 publications

Transcription elongator SPT6L regulates the occupancies of the SWI2/SNF2 chromatin remodelers SYD/BRM and nucleosomes at transcription start sites in Arabidopsis

Transcription elongator SPT6L regulates the occupancies of the SWI2/SNF2 chromatin remodelers SYD/BRM and nucleosomes at transcription start sites in Arabidopsis

Comparative Expression Profiling of Snf2 Family Genes During Reproductive Development and Stress Responses in Rice

Arabidopsis γ-H2A.X-INTERACTING PROTEIN participates in DNA damage response and safeguards chromatin stability

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