Arabidopsis CBF3 and DELLAs positively regulate each other in response to low temperature

Abstract

The C-repeat binding factor (CBF) is crucial for regulation of cold response in higher plants. In Arabidopsis, the mechanism of CBF3-caused growth retardation is still unclear. Our present work shows that CBF3 shares the similar repression of bioactive gibberellin (GA) as well as upregulation of DELLA proteins with CBF1 and -2. Genetic analysis reveals that DELLAs play an essential role in growth reduction mediated by CBF1, -2, -3 genes. The in vivo and in vitro evidences demonstrate that GA2-oxidase 7 gene is a novel CBF3 regulon. Meanwhile, DELLAs contribute to cold induction of CBF1, -2, -3 genes through interaction with jasmonate (JA) signaling. We conclude that CBF3 promotes DELLAs accumulation through repressing GA biosynthesis and DELLAs positively regulate CBF3 involving JA signaling. CBFs and DELLAs collaborate to retard plant growth in response to low temperature.

Changes in apoplastic peroxidase activity and cell wall composition are associated with cold‐induced morpho‐anatomical plasticity of wheat leaves

“…) and jasmonic acid (Zhou et al . ). Thus, the dwarf phenotype in winter varieties growing at low temperature has been related to some of these factors.…”

“…The known mechanisms underlying cold-induced signalling include post-transcriptional and post-translational key regulators, e.g. CBF/ DREB1 and phytohormones like ABA, auxin, gibberellic acid (GA; Kurepin et al 2013) and jasmonic acid (Zhou et al 2017). Thus, the dwarf phenotype in winter varieties growing at low temperature has been related to some of these factors.…”

Crosstalk of PIF4 and DELLA modulates CBF transcript and hormone homeostasis in cold response in tomato

“…Third, SlPIF4 directly bound to the G‐box of the CBF promoters in vitro and was associated with the promoters of the CBF genes in vivo (Figures d–g and S6). Finally, SlPIF4 promoted ABA and JA accumulation in tomato plants under cold stress (Figures and S7), which were positive regulators in cold stress (Wang et al , ), and enhanced the degradation of GA (Figures and S7), which was a negative regulator in cold tolerance (Achard et al , ; Zhou et al , ). Collectively, our results indicate that SlPIF4 works as a positive regulator of L‐R/FR‐induced cold tolerance in tomato plants that directly activates the transcription of CBF genes and regulates phytohormone homeostasis.…”

“…However, L‐R/FR failed to induce Fv/Fm values and CBF‐pathway gene ( CBF1 and COR413‐like ) expression in the SlGAI4‐ silenced plants (Figures b,c and S10b). Moreover, it has been reported that CBF and DELLAs positively regulate each other in response to low temperature in Arabidopsis (Achard et al , ; Zhou et al , ). Taken together, these results demonstrate that SlPIF4 also directly activated SlGAI4 , which acts as a positive regulator in the regulation of CBF1 gene expression and L‐R/FR‐induced cold tolerance in tomato plants during cold stress.…”

“…SlPIF4 directly binds the promoter of SlGAI4 and activates its transcription during cold stress DELLA proteins are the key repressors of almost all GA responses (Daviere and Achard, 2016) and play critical roles in the plant cold response (Achard et al, 2008;Zhou et al, 2017). There are ten DELLA genes (GA-INSENSITIVE, SlGAIs) in tomato, which were identified by phylogenetic analysis of tomato DELLA family genes ( Figure S8a).…”