Arabidopsis CAMTA3/SR1 is involved in drought stress tolerance and ABA signaling

Zeng, Houqing; Wu, Haicheng; Wang, Guoping; Dai, Senhuan; Zhu, Qiuqing; Yi, Keke; Du, Liqun

doi:10.1016/j.plantsci.2022.111250

Cited by 12 publications

(7 citation statements)

References 93 publications

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“…The MADS-box family is well known to play key roles in flower development (Bowman et al ., 1989; Coen & Meyerowitz, 1991; Weigel & Meyerowitz, 1994). Members of the CAMTA family are involved in drought stress tolerance, response to cold, and defense response in Arabidopsis (Zeng et al ., 2022; Yuan et al ., 2018). It has been documented that members of the MYB family play a role in the abiotic stress response and xylem development among others (Xiao et al ., 2021; Wang et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

Identification of genes involved in Kranz anatomy evolution of non-model grasses using unsupervised machine learning

Prochetto,

Stegmayer,

Studer

et al. 2024

Preprint

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Kranz syndrome is a set of leaf anatomical and functional characteristics of species using C4 photosynthesis. The current model for the evolution of C4 photosynthesis from a C3 ancestor proposes a series of gradual anatomical changes followed by a biochemical adaptation of the C4 cycle enzymatic machinery. In this work, leaf anatomical traits from closely related C3, C4 and intermediate species (Proto-Kranz, PK) were analyzed together with gene expression data to discover potential drivers for the establishment of Kranz anatomy using unsupervised machine learning. Species-specific Self-Organizing Maps (SOM) were developed to group features (genes and phenotypic traits) into clusters (neurons) according to their expression along the leaf developmental gradient. The analysis with SOM allowed us to identify candidate genes as enablers of key anatomical traits differentiation related to the area of mesophyll (M) and bundle sheath (BS) cells, vein density, and the interface between M and BS cells. At the same time, we identified a small subset of genes that displaced together with the change in the area of the BS cell along evolution suggesting a salient role in the origin of Kranz anatomy in grasses.

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Section: Discussionmentioning

confidence: 99%

Identification of genes involved in Kranz anatomy evolution of non-model grasses using unsupervised machine learning

Prochetto,

Stegmayer,

Studer

et al. 2024

Preprint

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“…The highly associated motif DMCGCGKH closely resembles a motif targeted by signal-responsive (SR) calmodulin-binding protein to activate genes involved in ethylene signaling, abscisic acid signaling, and light signal perception 49 . The Arabidopsis CAMTA3/SR1 gene is involved in drought and abscisic acid responses, and induces stomatal closure, a trait important in some plant-pathogen interactions 50 . Two of the three cacao CAMTA/SR orthologs, Thecc.02G138500 and Thecc.02G205100, were classified into correlation Groups 10 and 23, respectively.…”

Section: Discussionmentioning

confidence: 99%

Cacao pod transcriptome profiling of seven genotypes identifies features associated with post-penetration resistance to Phytophthora palmivora

Baruah,

Shao,

Ali

et al. 2024

Sci Rep

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The oomycete Phytophthora palmivora infects the fruit of cacao trees (Theobroma cacao) causing black pod rot and reducing yields. Cacao genotypes vary in their resistance levels to P. palmivora, yet our understanding of how cacao fruit respond to the pathogen at the molecular level during disease establishment is limited. To address this issue, disease development and RNA-Seq studies were conducted on pods of seven cacao genotypes (ICS1, WFT, Gu133, Spa9, CCN51, Sca6 and Pound7) to better understand their reactions to the post-penetration stage of P. palmivora infection. The pod tissue-P. palmivora pathogen assay resulted in the genotypes being classified as susceptible (ICS1, WFT, Gu133 and Spa9) or resistant (CCN51, Sca6 and Pound7). The number of differentially expressed genes (DEGs) ranged from 1625 to 6957 depending on genotype. A custom gene correlation approach identified 34 correlation groups. De novo motif analysis was conducted on upstream promoter sequences of differentially expressed genes, identifying 76 novel motifs, 31 of which were over-represented in the upstream sequences of correlation groups and associated with gene ontology terms related to oxidative stress response, defense against fungal pathogens, general metabolism and cell function. Genes in one correlation group (Group 6) were strongly induced in all genotypes and enriched in genes annotated with defense-responsive terms. Expression pattern profiling revealed that genes in Group 6 were induced to higher levels in the resistant genotypes. An additional analysis allowed the identification of 17 candidate cis-regulatory modules likely to be involved in cacao defense against P. palmivora. This study is a comprehensive exploration of the cacao pod transcriptional response to P. palmivora spread after infection. We identified cacao genes, promoter motifs, and promoter motif combinations associated with post-penetration resistance to P. palmivora in cacao pods and provide this information as a resource to support future and ongoing efforts to breed P. palmivora-resistant cacao.

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“…Our studies have shown that CAMTA3 functions as a negative regulator for salt tolerance in Arabidopsis [25]. On the other hand, CAMTA3 acts as a positive regulator for cold and drought stress response as well as defense against insect herbivory and wound-induced response [20,28,29]. CAMTA3 works in concert with CAMTA1, CAMTA2 and CAMTA5 to increase plant freezing tolerance by inducing the expression of the CRT/DRE-Binding Factor (CBF) genes and other cold-induced genes [24,26,30].…”

Section: Introductionmentioning

confidence: 90%

“…In Arabidopsis thaliana (referred to as Arabidopsis henceforth), there are six CAMTAs (AtCAMTA1 to AtCAMTA6, referred to as CAMTA1 to CAMTA6 henceforth), which are differentially expressed in response to multiple stresses [6,10,16]. Among these, CAMTA3 (also known as signal-responsive1, SR1), is the most studied member and regulates diverse biotic and abiotic stress responses, as well as thigmomorphogenesis [8,[17][18][19][20][21][22]. Several transcriptomic studies comparing WT and camta3 mutants revealed a dual regulatory role for CAMTA3 in multiple cellular pathways [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

DNA-Binding Activity of CAMTA3 Is Essential for Its Function: Identification of Critical Amino Acids for Its Transcriptional Activity

Prasad

Abdel-Hameed

Jiang

et al. 2023

Cells

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Calmodulin-binding transcription activators (CAMTAs), a small family of highly conserved transcription factors, function in calcium-mediated signaling pathways. Of the six CAMTAs in Arabidopsis, CAMTA3 regulates diverse biotic and abiotic stress responses. A recent study has shown that CAMTA3 is a guardee of NLRs (Nucleotide-binding, Leucine-rich repeat Receptors) in modulating plant immunity, raising the possibility that CAMTA3 transcriptional activity is dispensable for its function. Here, we show that the DNA-binding activity of CAMTA3 is essential for its role in mediating plant immune responses. Analysis of the DNA-binding (CG-1) domain of CAMTAs in plants and animals showed strong conservation of several amino acids. We mutated six conserved amino acids in the CG-1 domain to investigate their role in CAMTA3 function. Electrophoretic mobility shift assays using these mutants with a promoter of its target gene identified critical amino acid residues necessary for DNA-binding activity. In addition, transient assays showed that these residues are essential for the CAMTA3 function in activating the Rapid Stress Response Element (RSRE)-driven reporter gene expression. In line with this, transgenic lines expressing the CG-1 mutants of CAMTA3 in the camta3 mutant failed to rescue the mutant phenotype and restore the expression of CAMTA3 downstream target genes. Collectively, our results provide biochemical and genetic evidence that the transcriptional activity of CAMTA3 is indispensable for its function.

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Arabidopsis CAMTA3/SR1 is involved in drought stress tolerance and ABA signaling

Cited by 12 publications

References 93 publications

Identification of genes involved in Kranz anatomy evolution of non-model grasses using unsupervised machine learning

Identification of genes involved in Kranz anatomy evolution of non-model grasses using unsupervised machine learning

Cacao pod transcriptome profiling of seven genotypes identifies features associated with post-penetration resistance to Phytophthora palmivora

DNA-Binding Activity of CAMTA3 Is Essential for Its Function: Identification of Critical Amino Acids for Its Transcriptional Activity

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