Arabidopsis AtPARK13, Which Confers Thermotolerance, Targets Misfolded Proteins

Basak, Indranil; Pal, Ramavati; Patil, Ketan S.; Dunne, Aisling; Ho, Hsin-Pin; Lee, Sungsu; Peiris, Diluka; Maple‐Grødem, Jodi; Odell, Mark; Chang, Emmanuel J.; Larsen, Jan Petter; Møller, Simon Geir

doi:10.1074/jbc.m114.548156

Cited by 18 publications

(13 citation statements)

References 41 publications

(55 reference statements)

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“…DEG14/PARK13 (At5g27660) was the only member of the DEG/HtrA (high-temperature requirement A) protease family involved in protein quality control [ 116 ] that was upregulated by allyl-ITC. DEG14/PARK13 is heat stress-induced, and confers thermotolerance by degrading misfolded protein targets [ 117 ]. The heat-induced gene At5g53400 encoding the noncanonical small heat shock protein Bobber 1 (BOB1) required for thermotolerance and the duplicated gene BOB2 (At4g27890) were induced by allyl-ITC at 1 h and 9 h [ 118 ].…”

Section: Resultsmentioning

confidence: 99%

Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana

et al. 2016

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BackgroundIsothiocyanates (ITCs) are degradation products of the plant secondary metabolites glucosinolates (GSLs) and are known to affect human health as well as plant herbivores and pathogens. To investigate the processes engaged in plants upon exposure to isothiocyanate we performed a genome scale transcriptional profiling of Arabidopsis thaliana at different time points in response to an exogenous treatment with allyl-isothiocyanate.ResultsThe treatment triggered a substantial response with the expression of 431 genes affected (P < 0.05 and log2 ≥ 1 or ≤ -1) already after 30 min and that of 3915 genes affected after 9 h of exposure, most of the affected genes being upregulated. These are involved in a considerable number of different biological processes, some of which are described in detail: glucosinolate metabolism, sulphate uptake and assimilation, heat stress response, oxidative stress response, elicitor perception, plant defence and cell death mechanisms.ConclusionExposure of Arabidopsis thaliana to vapours of allyl-isothiocyanate triggered a rapid and substantial transcriptional response affecting numerous biological processes. These include multiple stress stimuli such as heat stress response and oxidative stress response, cell death and sulphur secondary defence metabolism. Hence, effects of isothiocyanates on plants previously reported in the literature were found to be regulated at the gene expression level. This opens some avenues for further investigations to decipher the molecular mechanisms underlying the effects of isothiocyanates on plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3039-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana

et al. 2016

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“…One missed trypsin cleavage per peptide was allowed and an initial mass tolerance of 0.3 Da was used in all searches. Complete carboxyamidation of cysteine sulfhydryls and partial oxidation of methionine were assumed [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

A Proteomics Approach to Investigate miR-153-3p and miR-205-5p Targets in Neuroblastoma Cells

et al. 2015

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MicroRNAs are key regulators associated with numerous diseases. In HEK293 cells, miR-153-3p and miR-205-5p down-regulate alpha-synuclein (SNCA) and Leucine-rich repeat kinase 2 (LRRK2), two key proteins involved in Parkinson’s disease (PD). We have used two-dimensional gel electrophoresis (2D-PAGE) coupled to mass spectrometry (MS) to identify a spectrum of miR-153-3p and miR-205-5p targets in neuronal SH-SY5Y cells. We overexpressed and inhibited both microRNAs in SH-SY5Y cells and through comparative proteomics profiling we quantified ~240 protein spots from each analysis. Combined, thirty-three protein spots were identified showing significant (p-value < 0.05) changes in abundance. Modulation of miR-153-3p resulted in seven up-regulated proteins and eight down-regulated proteins. miR-205 modulation resulted in twelve up-regulated proteins and six down-regulated proteins. Several of the proteins are associated with neuronal processes, including peroxiredoxin-2 and -4, cofilin-1, prefoldin 2, alpha-enolase, human nucleoside diphosphate kinase B (Nm23) and 14-3-3 protein epsilon. Many of the differentially expressed proteins are involved in diverse pathways including metabolism, neurotrophin signaling, actin cytoskeletal regulation, HIF-1 signaling and the proteasome indicating that miR-153-3p and miR-205-5p are involved in the regulation of a wide variety of biological processes in neuroblastoma cells.

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“…According to sequence-based predictions and previous experimental evidence, eight of the Deg proteases of Arabidopsis may be localized in mitochondria: DEG3, DEG4, DEG6, DEG7, DEG10, DEG11, DEG12, and DEG14 (Schuhmann and Adamska, 2012; Basak et al , 2014; Tanz et al , 2014). For DEG3 , DEG4 , and DEG12 , evidence of gene expression has been obtained only by microarray analyses, while DEG6 transcripts were hardly ever detected even in RNA-seq experiments [eFP Browser (Winter et al , 2007), data not shown].…”

Section: Introductionmentioning

confidence: 94%

DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis

Huber

Jakobs

Mishra

et al. 2019

Journal of Experimental Botany

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Maintaining mitochondrial proteome integrity is especially important under stress conditions to ensure a continued ATP supply for protection and adaptation responses in plants. Deg/HtrA proteases are important factors in the cellular protein quality control system, but little is known about their function in mitochondria. Here we analyzed the expression pattern and physiological function of Arabidopsis thaliana DEG10, which has homologs in all photosynthetic eukaryotes. Both expression of DEG10:GFP fusion proteins and immunoblotting after cell fractionation showed an unambiguous subcellular localization exclusively in mitochondria. DEG10 promoter:GUS fusion constructs showed that DEG10 is expressed in trichomes but also in the vascular tissue of roots and aboveground organs. DEG10 loss-of-function mutants were impaired in root elongation, especially at elevated temperature. Quantitative proteome analysis revealed concomitant changes in the abundance of mitochondrial respiratory chain components and assembly factors, which partially appeared to depend on altered mitochondrial retrograde signaling. Under field conditions, lack of DEG10 caused a decrease in seed production. Taken together, our findings demonstrate that DEG10 affects mitochondrial proteostasis, is required for optimal root development and seed set under challenging environmental conditions, and thus contributes to stress tolerance of plants.

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Arabidopsis AtPARK13, Which Confers Thermotolerance, Targets Misfolded Proteins

Cited by 18 publications

References 41 publications

Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana

Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana

A Proteomics Approach to Investigate miR-153-3p and miR-205-5p Targets in Neuroblastoma Cells

DEG10 contributes to mitochondrial proteostasis, root growth, and seed yield in Arabidopsis

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