Ara H1 peanut allergen detection using a labelled electrochemical aptasensor based on GO-COOH@bimetallic composite platform

Melinte, Gheorghe; Hosu, Oana; Cristea, Cécilia; Marrazza, Giovanna

doi:10.1016/j.foodchem.2022.134074

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Sequence changes are common at the 5 and 3 ends and generally consist of the insertion of an active functionality. They promote a greater binding affinity, and consequently more selectivity for the analytes [29,[87][88][89].…”

Section: Nanomaterial-modified Electrochemical Aptasensorsmentioning

confidence: 99%

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Relevant Aspects in the Development of Electrochemical Aptasensors for the Determination of Antibiotics—A Review

da Silva,

Pereira

2023

Electrochem

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Aptamers are three-dimensional structures of DNA or RNA that present high affinity and selectivity to specific targets, obtained through in vitro screening. Aptamers are used as biological recognizers in electrochemical biosensors, the so-called aptasensors, providing greater specificity in recognizing the most diverse analytes. Electrochemical aptasensors have extremely relevant characteristics, such as high sensitivity, low cost compared to other biorecognizers such as antibodies, and excellent compatibility, being considered one of the most promising alternative methods in several areas, such as biomedical diagnosis and monitoring environmental contaminants. In this sense, the present work reviews the relevant aspects of methodologies based on electrochemical aptasensors and their applications in determining antibiotics, seeking to foster innovation in electrochemical biosensors.

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Section: Nanomaterial-modified Electrochemical Aptasensorsmentioning

confidence: 99%

“…Aptasensors can be used in several areas, such as clinical diagnosis [24][25][26][27], the food industry [28][29][30], and environmental monitoring of contaminating species [31][32][33][34], with high precision.…”

Section: Introductionmentioning

confidence: 99%

Relevant Aspects in the Development of Electrochemical Aptasensors for the Determination of Antibiotics—A Review

da Silva,

Pereira

2023

Electrochem

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“…While antibodies have been widely applied as recognition elements, the application of aptamers has several unique advantages, such as economical and reproducible synthesis, simple modification, high thermal stability, and programmability. , However, the application of aptamers has fallen short of expectations due to their unsatisfactory binding ability in complex application environments. , Engineering aptamers into multivalent aptamers can directly enhance the binding performance due to the repeated recognition unit and “cooperative” bound configuration. − In particular, designing multivalent aptamers based on three-dimensional nanostructures can not only enhance the binding ability of recognition elements but also improve their stability in complex environments. , Although Ara h1-specific aptamers have been successfully used and verified in the construction of Ara h1-targeted aptasensors, − engineering Ara h1-specific aptamers to further improve the application performance of aptamers has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Ratiometric Fluorescence Aptasensor of Allergen Protein Based on Multivalent Aptamer-Encoded DNA Flowers as Fluorescence Resonance Energy Transfer Platform

Qi,

Dong,

Hamed

et al. 2024

Anal. Chem.

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Life-threatening allergic reactions to food allergens, particularly peanut protein Ara h1, are a growing public health concern affecting millions of people worldwide. Thus, accurate and rapid detection is necessary for allergen labeling and dietary guidance and ultimately preventing allergic incidents. Herein, we present a novel ratiometric fluorescence aptasensor based on multivalent aptamer-encoded DNA flowers (Mul-DNFs) for the high-stability and sensitive detection of allergen Ara h1. The flower-shaped Mul-DNFs were spontaneously packaged using ultralong polymeric DNA amplicons driven by a rolling circle amplification reaction, which contains a large number of Ara h1 specific recognition units and has excellent binding properties. Furthermore, dual-color fluorescence-labeled Mul-DNFs probes were developed by hybridizing them with Cy3-and Cy5-labeled complementary DNA (cDNA) to serve as a ratiometric fluorescence aptasensor platform based on fluorescence resonance energy transfer. Benefiting from the combined merits of the extraordinary synergistic multivalent binding ability of Mul-DNFs, the excellent specificity of the aptamer, and the sensitivity of the ratiometric sensor to avoid exogenous interference. The developed ratiometric aptasensor showed excellent linearity (0.05−2000 ng mL −1 ) with a limit of detection of 0.02 ng mL −1 . Additionally, the developed ratiometric fluorescence aptasensor was utilized for quantifying the presence of Ara h1 in milk, infant milk powder, cookies, bread, and chocolate with recoveries of 95.7−106.3%. The proposed ratiometric aptasensor is expected to be a prospective universal aptasensor platform for the rapid, sensitive, and accurate determination of food and environmental hazards.

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Discovery, verification, and validation of walnut protein marker peptides using LC-MS approaches

Kim

Lee

et al. 2023

Food Chemistry

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Ara H1 peanut allergen detection using a labelled electrochemical aptasensor based on GO-COOH@bimetallic composite platform

Cited by 6 publications

References 25 publications

Relevant Aspects in the Development of Electrochemical Aptasensors for the Determination of Antibiotics—A Review

Relevant Aspects in the Development of Electrochemical Aptasensors for the Determination of Antibiotics—A Review

Ratiometric Fluorescence Aptasensor of Allergen Protein Based on Multivalent Aptamer-Encoded DNA Flowers as Fluorescence Resonance Energy Transfer Platform

Discovery, verification, and validation of walnut protein marker peptides using LC-MS approaches

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