Aquisphaera insulae sp. nov., a new member in the family Isosphaeraceae, isolated from the floating island of Loktak lake and emended description of the genus Aquisphaera

Kumar, Gaurav; Lhingjakim, Khongsai L; Jagadeeshwari, Uppada; Ahamad, Shabbir; Kumar, Dhanesh; Kashif, Gulam Mohammad; Sasikala, Ch.; Ramana, Chintalapati Venkata

doi:10.1007/s10482-021-01615-6

Cited by 11 publications

(4 citation statements)

References 46 publications

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“…. The two known aquatic species of Aquisphaera have an incomplete nitrification pathway, thereby lacking the gene coding for ammonia monooxygenase [ 85 ]. Russula has acquired an evolutionarily conserved genetic profile for N acquisition and it specializes in ammonium rather than NO 2 uptake [ 86 ].…”

Section: Discussionmentioning

confidence: 99%

Fungal and bacterial communities and their associations in snow-free and snow covered (sub-)alpine Pinus cembra forest soils

Probst,

Telagathoti,

Mandolini

et al. 2024

Environmental Microbiome

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Background In Europe, Pinus cembra forests cover subalpine and alpine areas and they are of high conservational and ecological relevance. These forests experience strong seasonality with alternating snow-free and snow covered periods. Although P. cembra is known for mycorrhization and mycorrhizae usually involve fungi, plants and bacteria, the community compositions of fungi and bacteria and their associations in (sub-)alpine P. cembra forests remain vastly understudied. Here, we studied the fungal and bacterial community compositions in three independent (sub-)alpine P. cembra forests and inferred their microbial associations using marker gene sequencing and network analysis. We asked about the effect of snow cover on microbial compositions and associations. In addition, we propose inferring microbial associations across a range of filtering criteria, based on which we infer well justified, concrete microbial associations with high potential for ecological relevance that are typical for P. cembra forests and depending on snow cover. Results The overall fungal and bacterial community structure was comparable with regards to both forest locations and snow cover. However, occurrence, abundance, and diversity patterns of several microbial taxa typical for P. cembra forests differed among snow-free and snow covered soils, e.g. Russula, Tetracladium and Phenoliphera. Moreover, network properties and microbial associations were influenced by snow cover. Here, we present concrete microbial associations on genus and species level that were repeatedly found across microbial networks, thereby confirming their ecological relevance. Most importantly, ectomycorrhizal fungi, such as Basidioascus, Pseudotomentella and Rhizopogon, as well as saprobic Mortierella changed their bacterial association partners depending on snow cover. Conclusion This is the first study researching fungal-bacterial associations across several (sub-)alpine P. cembra forests. The poorly investigated influence of snow cover on soil fungi and bacteria, especially those mycorrhizing P. cembra roots, but also saprobic soil organisms, underlines the relevance of forest seasonality. Our findings highlight that the seasonal impact of snow cover has significant consequences for the ecology of the ecosystem, particularly in relation to mycorrhization and nutrient cycling. It is imperative to consider such effects for a comprehensive understanding of the functioning resilience and responsiveness of an ecosystem.

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Section: Discussionmentioning

confidence: 99%

Fungal and bacterial communities and their associations in snow-free and snow covered (sub-)alpine Pinus cembra forest soils

Probst,

Telagathoti,

Mandolini

et al. 2024

Environmental Microbiome

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“…The CMFRI is situated in Tamil Nadu in the south-eastern part of India,. At the time of sample collection, the sampling spots had a pH of 7.0-7.5 and a temperature of 22 • C. Algal mat samples were subjected to enrichment and cultivation in a medium [20,24] containing (per litre distilled water; pH 7.0): N-acetylglucosamine, 2.0 g; KH 2 PO 4 , 0.1 g; peptone, 0.1 g; yeast extract, 0.1 g; vitamin solution, 10 mL; Hutner's basal salts, 20 mL, prepared in distilled water. The antibiotics streptomycin, 400 mg/L, ampicillin, 200 mg/L and cycloheximide, 25 mg/L were added to the medium.…”

Section: Habitat and Isolationmentioning

confidence: 99%

“…Recent studies have shown that India harbours a rich planctomycetal diversity which is reflected by the isolation of several axenic cultures from different aquatic and terrestrial habitats [20][21][22][23][24]. In this study, we describe a novel species belonging to the genus Gemmata based on two novel strains that were independently isolated from two distinct and distantly located ecosystems (ca.…”

Section: Introductionmentioning

confidence: 99%

Gemmata algarum, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity

Kumar,

Kallscheuer,

Kashif

et al. 2023

Marine Drugs

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Axenic cultures of two strains, JC673T and JC717, both belonging to the phylum Planctomycetota, were isolated from distinct geographical locations in India. Strain JC673T was obtained from algal mats of a wetland situated in the state of Kerala, India, while strain JC717 originated from the Central Marine Fisheries Research Institute (CMFRI), state of Tamil Nadu, India. The two strains share 99.9% 16S rRNA gene sequence similarity and are most closely related to Gemmata obscuriglobus UQM 2246T (99.3% 16S rRNA gene sequence identity). The newly isolated strains are Gram-negative, grow aerobically and tolerate up to 4% (w/v) NaCl and a pH of up to 9.0. Cells are spherical and form pink-pigmented colonies. The respiratory quinone is MK-6. Major fatty acids are C18:0, C16:1ω5c and C16:0. Polar lipids include phosphatidylcholine, phosphatidylethanolamine, several unidentified amino lipids, unidentified phospholipids, additional unidentified lipids, and an unidentified choline lipid. The polyamine spermidine is produced by the two strains. The strains have a genome size of about 8.2 Mb with a DNA G+C content of 67.6%. Solvent-based culture extracts of the isolates showed antimicrobial activity against three bacterial test strains. Their phylogenetic position along with differences in morphological, physiological, and genomic features support the classification as a new species of the genus Gemmata, for which we propose the name Gemmata algarum sp. nov. Strain JC673T (=KCTC 72851T = NBRC 114340T) and JC717 are the type and non-type strain of the new species, respectively.

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“…The current known diversity of the phylum Planctomycetota is thus still very incomplete. Several isolation efforts in recent years have led to a substantial number of strains to be brought into pure culture and the formal description of more than half of the currently known species [4,14,[32][33][34][35][36][37][38][39][40][41][42][43][44][45][46]. Taxonomic rearrangements within the phylum were also recently made [47].…”

Section: Introductionmentioning

confidence: 99%

Rubinisphaera margarita sp. nov., a novel planctomycete isolated from marine sediments collected in the Portuguese north coast

Vitorino

Lobo‐da‐Cunha

Vasconcelos

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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The phylum Planctomycetota is constituted by bacteria with unique features that are well adapted to a vast range of habitats. Here, we describe a novel planctomycete isolated from marine sediments collected on a beach in Matosinhos (Portugal) using an iChip-based culturing technique. Strain ICM_H10T forms beige-coloured colonies in modified M14 medium and its cells are spherical to ovoid in shape, stalked, rosette-forming and showing motility in a phase of the life cycle. Transmission electron microscopy observations showed a typical planctomycetal cell plan and cell division by budding. This strain requires salt for growth and grows in the range of 2.0–5.0 % (w/v) NaCl, from 20 to 37 °C, within a pH of 6.0–9.0 and is able to use diverse nitrogen and carbon sources. It is heterotrophic, aerobic and capable of microaerobic growth. This strain has a genome size of approximately 6.0 Mb and a G+C content of 58.1 mol%. A 16S rRNA gene-based phylogenetic analysis supports the association of strain ICM_H10T to the phylum Planctomycetota and the family Planctomycetaceae , as it shares only 96.8 and 96.4% similarity to its closest relatives Rubinisphaera italica Pan54T and Rubinisphaera brasiliensis IFAM 1448T, respectively. Other phylogenetic markers also support the separation of this strain into a novel species. Morphological, physiological and genomic comparisons between strain ICM_H10T and its closest relatives strongly suggest that ICM_H10T represents a new species of the genus Rubinisphaera , for which we propose the name Rubinisphaera margarita sp. nov., with ICM_H10T (=CECT 30326T=LMG 32234T) as type strain.

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Aquisphaera insulae sp. nov., a new member in the family Isosphaeraceae, isolated from the floating island of Loktak lake and emended description of the genus Aquisphaera

Cited by 11 publications

References 46 publications

Fungal and bacterial communities and their associations in snow-free and snow covered (sub-)alpine Pinus cembra forest soils

Fungal and bacterial communities and their associations in snow-free and snow covered (sub-)alpine Pinus cembra forest soils

Gemmata algarum, a Novel Planctomycete Isolated from an Algal Mat, Displays Antimicrobial Activity

Rubinisphaera margarita sp. nov., a novel planctomycete isolated from marine sediments collected in the Portuguese north coast

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