Aquifer Discharge Drives Microbial Community Change in Karst Estuaries

Microbial Function and Hydrochemistry within a Stratified Anchialine Sinkhole: A Window into Coastal Aquifer Interactions

“…The salinity and sulfate content suggests that water in the hypoxic layer is derived from the shallower part of the coastal mixing zone. Water with similar properties has been found in the Double Keyhole Spring system [13] and wells at least 160 m deep (Table 4), which penetrate the Avon Park Formation according to the well description by the South Florida Water Management District (SWFMD). Despite this comparison, we are currently unable to determine whether the sulfate is derived from gypsum dissolution from deeper within the aquifer or from saltwater from the Gulf of Mexico.…”

“…Statistical analyses of the replicate microbial community and hydrochemical data were performed using Primer v7/Permanova + statistical software (Primer-E Ltd., Albany, New Zealand). Abiotic data were transformed (log X + 1), normalized and clustered using Euclidean distance [13]. The OTU abundance of each replicate was used for the top 2000 OTUs for Bacteria and the top 200 OTUs for Archaea in our dataset for Primer-E biological analysis.…”

“…The OTU abundance of each replicate was used for the top 2000 OTUs for Bacteria and the top 200 OTUs for Archaea in our dataset for Primer-E biological analysis. Biological data were square-root transformed and clustered using Brays-Curtis similarity [13]. Biological and abiotic replicate data were analyzed using principal coordinate analysis (PCoA).…”

“…Biological and abiotic replicate data were analyzed using principal coordinate analysis (PCoA). A Bio-Env (BEST) analysis was performed to determine relationships between biotic and abiotic datasets [13]. Principal coordinate analyses were utilized in order to avoid multicollinearity, non-linearity and non-normality within the biological and abiotic data.…”

Spatial and temporal dynamics of bacterioplankton community composition in a subtropical dammed karst river of southwestern China

“…Karst rivers are commonly regulated by damming, yet the influence of these dams on changes in hydrological series of water discharge is negative or positive (Miao, Ni, Borthwick, & Yang, ). Although the diversity and dynamics of microbes in karst springs (Farnleitner et al., ; Ohad et al., ; Savio et al., ), unsaturated and saturated karst aquifers (Cooper et al., ; Gray & Engel, ; Johnson et al., ; Lin et al., ; Menning et al., ), and water pools (Shabarova et al., ) as well as in groundwater‐surface water exchange systems (Li, Song, et al., ) have been discussed in the literature, much less attention has been paid to the structure of bacterioplankton communities in dammed karst rivers. In addition, previous studies on bacterioplankton communities in the canyon‐shaped and meso‐eutrophic Rimov Reservoir (Simek et al., ), the dammed Ebro River (Ruiz‐González, Proia, Ferrera, Gasol, & Sabater, ), and the rivers controlled by the Three Gorges Dam (Huang et al., ; Li, Lu, et al., ; Yan et al., ) did not include the seasonal variation or depth dynamics in bacterioplankton.…”