Aqueous two-phase systems containing self-associating block copolymers

Integrated method of thermosensitive triblock copolymer–salt aqueous two phase extraction and dialysis membrane separation for purification of lycium barbarum polysaccharide

“…The incompatibility between EOPOEO and polysaccharides (e.g. dextran) can lead to the formation of ATPS (Svensson, Berggren, Veide, & Tjerneld, 1999;Tada, Loh, & Pessôa-Filho, 2004), that is to say, EOPOEO and polysaccharides would enrich in the opposite phases. As for the EOPOEO-salt ATPS, with increasing salt concentration, the polymer concentration in the top phase and the salt concentration in the bottom would increase (Haraguchi, Mohamed, & Loh, 2004).…”

Phase equilibrium in aqueous two-phase systems containing ethylene oxide–propylene oxide block copolymers and dextran

“…Moreover, there is a remarkable change in the phase diagrams when temperature is raised from 5 to 40 • C, which was ascribed to changes on the aggregation state of the copolymer molecules. Further work by the same group was reported by Svensson et al [12], who investigated the partitioning behavior of aminoacids, dipeptides and tripeptides in ATPS formed by dextran T500 and the block copolymer P105, and by Svensson et al [13], who determined the partition coefficients of some proteins in ATPS formed by dextran T500 and either F68 or P105.…”

Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification

“…Since pIs (isoelectric points) for TRY and LYS are 10.4 and 11.0, respectively [13], both proteins are positively charged in all the pHs assayed. Fig.…”

“…2 shows the BSA partitioning behaviour in the different buffer media, according to Table 1. K r values lower [13], therefore it will be positively charged at a pH lower than pI and negatively charged at a pH higher than pI.…”

“…A (NaPi) and B (NaPi + NaClO 4 ) media: BSA has a negative charge of approximately −18 [13] in both media since pH is 7.40. As can be seen the ClO 4 − presence (B medium) induces a decrease of K r with respect to the value in solutions with only NaPi (A medium).…”