Aqueous two-phase poly(ethylene glycol)–sodium polyacrylate system for amyloglucosidase purification: Equilibrium diagrams and partitioning studies

Study of Alpha-Amylase Obtained by Solid State Fermentation of Cassava Residue in Aqueous Two-Phase Systems

“…This indicates that the ATPSs formed from PEG and potassium phosphate buffer are suitable for the separation of α-amylase, since they promoted partitioning of this biomolecule without significant loss of biological activity. Alcântara et al (2013), studying the extraction and purification of amyloglucosidase with ATPSs formed by polyethylene glycol and sodium polyacrylate, noted that the partition coefficient of amyloglucosidase activity decreased when the pH was greater than 6.5.…”

Separation of phenolic compounds by centrifugal partition chromatography

“…Polymer-based ABS are commonly composed of polyethylene glycol (PEG) and dextran 21–23. However, due to the high cost of dextran and the high viscosity that PEG-dextran-based systems present,24 other polymer combinations have been proposed 25–27. Amongst these, sodium polyacrylate (NaPA)-PEG-based ABS appeared as a promising combination of phase-forming components that have been successfully applied in the purification of a wide variety of biomolecules 18,28,29.…”

“…Even though there are still different visions on the molecular-level phenomenon which rules the phase separation, these systems have shown some interesting results regarding the purification of biomolecules 27. In general, in PEG-NaPA systems, the manipulation of the electrolyte nature affects the biomolecules partition and their selectivity to one of the phases, which are a result of specific interactions occurring between the phase-forming components, the electrolyte, and the target biomolecules or their contaminants 20,25,29. Biomolecules of higher complexity and molecular weight, like cytochrome c,30 hemoglobin,31 lysozyme31 and protease,28 and simpler biomolecules, such as clavulanic29 and chloranilic acids30, were studied using PEG-NaPA-based ABS with different electrolytes.…”

Liquid–Liquid Equilibrium of Two-Phase Aqueous Systems Composed of PEG 400, Na2SO4, and Water at Different Temperatures and pH Values: Correlation and Thermodynamic Modeling

“…Aqueous two-phase systems have played an important role in the purification of several biological compounds (animal or plant cells, microorganisms, nucleic acids, enzymes, proteins, and pigments), ,− in removal of metal ions and sulfide minerals, , in nanoparticles recovery, and to remove organic pollutants from environments …”

“…Several equilibrium data for the polyethylene glycol and sodium sulfate systems are available in the literature, ,− including those formed by PEG 400 and sodium sulfate. , However, despite the many published studies with equilibrium data for the systems containing PEG 400 and sodium sulfate, there is no report on equilibrium data for these systems at temperatures of 293.15, 298.15, 303.15, and 308.15 K associated with low pH values of pH 3, 4. and 5. Conditions are optimal for the stability of certain biomolecules such as pigments and proteins that can be commercially used in the pharmaceutical and food industries. Among these pigments, it is important to mention anthocyanins and betalains that have their optimal stability at low pH values.…”