Aqueous two‐phase extraction using thermoseparating copolymer: a new system for phenolic compounds removal from hemicelullosic hydrolysate

Abstract

BACKGROUND: The hydrolysis of hemicellulosic material can provide liquor with high xylose concentration (which can be used as a fermentation medium) and phenolic compounds (Phs), potentially immunostimulating compounds. However, these hydrolysates must be detoxified in order to remove the Phs that can act as inhibitors in bioconversions.

Use of response surface methodology to evaluate the extraction of Debaryomyces hansenii xylose reductase by aqueous two-phase system

“…Aqueous two-phase systems (ATPSs) have been employed as an efficient tool in several biotechnological processes for the partition of proteins, among which ␣-and ␤-lactoalbumins [7], proteases [8] and ␣-toxin [9], nucleic acids, microorganisms, animal and plants cells [10,11], and even phenolic compounds present in hemicellulosic hydrolyzates [12]. Also, ATPSs were successfully used to purify ␣-galactosidase [6], glucose oxidase, ␤-galactosidase [13], ␣-amylase [14], xylanolytic complex [15] and chymosin [16].…”

Detoxification of Lignocellulosic Hydrolysates

“…Hahn -H ä gerdal et al 1981 ;Jarzebski et al 1992 ;Banik et al 2003 ;Hasmann et al 2008 Supercritical fl uid extraction Excellent biocompatibility; remove furans, phenolics, and aliphatic acids polarity dependently; high concentration factor of inhibitors Hahn -H ä gerdal et al 1981 ;Jarzebski et al 1992 ;Banik et al 2003 ;Hasmann et al 2008 Supercritical fl uid extraction Excellent biocompatibility; remove furans, phenolics, and aliphatic acids polarity dependently; high concentration factor of inhibitors …”

Improving glutathione extraction from crude yeast extracts by optimizing aqueous two-phase system composition and operation conditions

“…Aqueous two-phase systems (ATPS), which have been widely applied in the separation and purification of biomolecules, such as proteins, enzymes, and nucleic acids, is a promising alternative to many conventional processes because of its apparent advantages: simplicity (one-step separation), low energy consumption, ease of scale-up, and maintenance of biological activity [11,12]. ATPS is formed by mutual incompatibility of different polymers or polymer/ salt in aqueous solutions above critical concentrations.…”