Aqueous two-phase extraction as a platform in the biomanufacturing industry: Economical and environmental sustainability

Rosa, P.A.J.; Azevedo, Ana M.; Sommerfeld, Sven D.; Bäcker, W.; Aires-Barros, M. Raquel

doi:10.1016/j.biotechadv.2011.03.006

Cited by 144 publications

(100 citation statements)

References 26 publications

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“…More than 95% of all impurities were removed, however, at the expense of a very low BE recovery yield (34%). The high NaCl concentrations determined for this BE system (7.4 and 6.5% in the BP and TP, respectively) together with the high volume ratio used may explain why such a low recovery yield was obtained for this step, as previous studies have shown that high NaCl concentration was one of the driving forces for the IgG extraction into the PEG-rich TP [4,11,15,19,26].…”

Section: Back-extraction Studiesmentioning

confidence: 83%

“…Aqueous two-phase system (ATPS) has shown to be considerably advantageous in terms of process economy and operation, especially when processing high titer cell culture supernatants [13]. This liquid-liquid extraction (LLE) technology has been successfully reported in recent times as a primary stage unit operation in the downstream processing of several biological products including therapeutic proteins, such as antibodies [4,10,11,[15][16][17][18][19][20][21][22][23][24]. Its technical feasibility at large scale has been, however, demonstrated for the downstream processing of just few biological products [25][26][27].…”

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confidence: 99%

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Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to process

Rosa

Azevedo

Sommerfeld

et al. 2013

Biotechnology Journal

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An aqueous two‐phase extraction (ATPE) process based on a PEG/phosphate system was developed for the capture of human immunoglobulin G and successfully applied to a Chinese hamster ovary and a PER.C6® cell supernatant. A continuous ATPE process incorporating three different steps (extraction, back‐extraction, and washing) was set up and validated in a pump mixer‐settler battery. Most of the higher molecular weight cell supernatant impurities were removed during the extraction step, while most of the lower molecular weight impurities were removed during the subsequent steps. A global recovery yield of 80% and a final protein purity of more than 99% were obtained for the IgG purification from a CHO cell supernatant, representing a 155‐fold reduction in the protein/IgG ratio. For the purification of IgG from a PER.C6® cell supernatant, a global recovery yield of 100%, and a host cell protein purity were attained, representing a 22‐fold reduction in the host cell protein/IgG ratio. These results, thus, open promising perspectives for the application of the developed ATPE process as a platform for the capture of antibodies. In fact, this new process has shown the ability to successfully recover and purify different antibodies from distinct cell culture supernatants. This technology can also overcome some of the limitations encountered using the typical chromatographic processes, besides inherent advantages of scalability, process integration, capability of continuous operation, and economic feasibility.

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Section: Back-extraction Studiesmentioning

confidence: 83%

mentioning

confidence: 99%

Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to process

Rosa

Azevedo

Sommerfeld

et al. 2013

Biotechnology Journal

Self Cite

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“…Therefore, ATPS implementation is economically attractive due to its potential of reducing overall downstream costs [49]. Moreover, ATPS processes have been directly compared to chromatographic methods in terms of process economics and purification performance for monoclonal antibodies purifica- tion [50], where the potential of a low-cost purification process based on ATPS has been shown to be possible. UF is a technique widely used for the separation and purification of proteins and other biological compounds due to its ability to perform in mild temperature conditions, which implies that degradation of thermolabile compounds can be lessened or even avoided [51].…”

Section: High Robustness Techniques With Low Resolutionmentioning

confidence: 99%

Purification and Formulation of Xanthophyll for Pharmaceutical Use: Current Strategies and Future Trends

2015

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The increasing attention to biological activities of dietary xanthophylls demands the establishment of attractive and easy-to-implement purification protocols for their recovery from different sources. Bioengineering strategies that involve alternative isolation and purification techniques have gained interest since they offer practical benefits in comparison to traditional column chromatography methods. Furthermore, the use of these techniques has resulted in compound purities from 50 to 98 %. Besides purification, it is important to keep in mind that xanthophyll bioavailability is limited due to their low solubility in digestive fluids. Therefore, there is also the need to enhance xanthophyll bioavailability through formulations of the purified compounds. Several formulation techniques have been employed to obtain products for bioavailability or stability studies. However, there is a lack of studies that address the effect of the formulation upon the bioavailability and stability of these compounds. In the present review, emerging purification and formulation technologies that have shown potential for xanthophyll isolation and bioavailability enhancement are critically discussed. This analysis is presented in order to discuss the current trends in the purification and formulation technologies of xanthophylls, particularly for pharmaceutical use.

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“…With a raise in butyric acid content, we observed a decrease of the total sugar and nitrogen compounds gathered in the PEG-rich phase, the sodium sulfate-rich phase contained more salt formations with Na 2 SO 4 , sugar and nitrogen compounds. Therefore, the PEG-rich phase was adopted for the extraction [34]. Because the butyric acid obtained from the extraction was mixed with a acetic acid, we distilled it exploit in the different boiling points of the two acids, obtaining pure butyric acid (99.5%).…”

Section: Comparison Between the Composition Of The Peg-rich And Sodiumentioning

confidence: 99%

Study on extraction and separation of butyric acid from clostridium tyrobutyricum fermentation broth in PEG/Na2SO4 aqueous two-phase system

Yang

et al. 2015

Fluid Phase Equilibria

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Aqueous two-phase extraction as a platform in the biomanufacturing industry: Economical and environmental sustainability

Cited by 144 publications

References 26 publications

Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to process

Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to process

Purification and Formulation of Xanthophyll for Pharmaceutical Use: Current Strategies and Future Trends

Study on extraction and separation of butyric acid from clostridium tyrobutyricum fermentation broth in PEG/Na2SO4 aqueous two-phase system

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