Aqueous size exclusion chromatography in semimicro and micro-columns by newly synthesized monodisperse macroporous hydrophilic beads as a stationary phase

Gölgelioğlu, Çiğdem; Bayraktar, Aslıhan; Çelebі, Bekіr; Uğuzdoğan, Erdal; Tuncel, Alï

doi:10.1016/j.chroma.2011.12.043

Cited by 11 publications

(8 citation statements)

References 20 publications

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“…The transport properties of fluids imbibed in porous media play a key role in several research areas and determine the efficiency of processes, such as catalysis − and separation, , in energy and bioengineering fields. , Determination of accurate morphological features of porous media is a current challenge to material scientists as many properties of stationary supports depend not only on the void size distribution but also on their connectivity and liquid–surface interactions. Porous polymeric scaffolds have a wide range of applications and can be prepared with a controlled hierarchy of micro-, meso-, and macroporous spatial domains. − Because of the hydrophilic characteristic of these materials, water not only diffuses into pore structures but also swells the network, developing a layer of gel inside of the material.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Surface Interaction of Water Confined in Hierarchical Porous Polymers Induced by Hydrogen Bonding

et al. 2016

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Hierarchical porous polymer systems are increasingly applied to catalysis, bioengineering, or separation technology because of the versatility provided by the connection of mesopores with percolating macroporous structures. Nuclear magnetic resonance (NMR) is a suitable technique for the study of such systems as it can detect signals stemming from the confined liquid and translate this information into pore size, molecular mobility, and liquid-surface interactions. We focus on the properties of water confined in macroporous polymers of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly(EGDMA-co-HEMA)] with different amounts of cross-linkers, in which a substantial variation of hydroxyl groups is achieved. As soft polymer scaffolds may swell upon saturation with determined liquids, the use of NMR is particularly important as it measures the system in its operational state. This study combines different NMR techniques to obtain information on surface interactions of water with hydrophilic polymer chains. A transition from a surface-induced relaxation in which relaxivity depends on the pore size to a regime where the organic pore surface strongly restricts water diffusion is observed. Surface affinities are defined through the molecular residence times near the network surface.

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Section: Introductionmentioning

confidence: 99%

Enhanced Surface Interaction of Water Confined in Hierarchical Porous Polymers Induced by Hydrogen Bonding

et al. 2016

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“…On the other hand, the surface of poly(GDMA-co-GDGDA) particles had a sponge-like pore structure containing homogeneously distributed small macropores. The porous properties of poly(GDMA-co-GDGDA) beads were determined by inverse size-exclusion chromatography (ISEC) [25]. By using the pore-size distribution curve of poly(GDMA-co-GDGDA) beads, the results showed that the pore size lies in the range of 2-120 nm for when seed latex monomer ratio was 0.038 g mL −1 [25].…”

Section: Resultsmentioning

confidence: 99%

“…The porous properties of poly(GDMA-co-GDGDA) beads were determined by inverse size-exclusion chromatography (ISEC) [25]. By using the pore-size distribution curve of poly(GDMA-co-GDGDA) beads, the results showed that the pore size lies in the range of 2-120 nm for when seed latex monomer ratio was 0.038 g mL −1 [25]. The variation of column back-pressure with the flow rate change is given in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The poly(glycidyl metacrylate) seed latex was obtained by dispersion polymerization of GMA as 2.1 μm microspheres [24]. Typically, GMA (3 mL) and AIBN (0.24 g) were added into the organic phase obtained in sealed pyrex glass reactor by dissolving PVP K-30 (0.45 g) in Et-OH (30 mL).…”

Section: Preparation Of Seed Latexmentioning

confidence: 99%

“…Multistage swelling and polymerization technique was used for the synthesis of poly(GDMA-co-GDGDA) [25]. In the first stage, following the preparation of aqueous solution (60 mL) containing SDS (0.15 g) and PVA (0.8 g), the organic phase containing DBP (1.8 mL) and Cyc-OH (3.1 mL) was emulsified in the aqueous medium by sonication for 20 min.…”

Section: Synthesis Of Monodisperse Poly(gdma-co-gdgda) Particlesmentioning

confidence: 99%

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A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

Çelebі

2017

Acta Chromatographica

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Summary. New polyacrylate-based monosized-porous polymer beads were proposed as a stationary phase for the separation of polar compounds by microbore reversed-phase chromatography. For this purpose, monosized-porous poly(glycerol dimethacrylate-coglycerol-1,3-diglycerolate diacrylate), poly(GDMA-co-GDGDA), beads with hydroxyl functionality were synthesized by a modified seeded polymerization. The selected octadecylating agent, stearoyl chloride (SC), was covalently attached onto the hydrophilic beads via a direct, single stage reaction with a simple synthetic route. SC attachedpoly(GDMA-co-GDGDA) beads were slurry-packed into the microbore columns and used as separation medium microbore reversed-phase chromatography. The stationary phase was used for separation of alkylbenzenes and polar analytes by micro reversedphase chromatography, using mobile phases with low acetonitrile content. Theoretical plate number (TPN) values up to 12,000 plates m −1 and 10,000 plates m −1 for alkylbenzenes and polar analytes, respectively, were achieved. The results also showed that poly(GDMA-co-GDGDA) hydrogel beads are a promising starting material for a number of chromatographic applications like reversed-phase (RP) chromatography, hydrophilic interaction chromatography (HILIC), ion-exchange chromatography (IEC), and affinity chromatography with a single-stage surface modification.

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A New Stationary Phase for Hydrophilic Interaction Chromatography: Polyacrylate-Based Hydrophilic, Monosized-Porous Beads with Zwitterionic Molecular Brushes

et al. 2014

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Aqueous size exclusion chromatography in semimicro and micro-columns by newly synthesized monodisperse macroporous hydrophilic beads as a stationary phase

Cited by 11 publications

References 20 publications

Enhanced Surface Interaction of Water Confined in Hierarchical Porous Polymers Induced by Hydrogen Bonding

Enhanced Surface Interaction of Water Confined in Hierarchical Porous Polymers Induced by Hydrogen Bonding

A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

A New Stationary Phase for Hydrophilic Interaction Chromatography: Polyacrylate-Based Hydrophilic, Monosized-Porous Beads with Zwitterionic Molecular Brushes

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