Size Exclusion Chromatography 1989
DOI: 10.1007/978-1-4615-7861-1_7
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Aqueous size exclusion chromatography

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Cited by 6 publications
(7 citation statements)
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“…Polyelectrolyte-gel adsorption can be reduced, in general, by addition of a low molar mass electrolyte to the eluant (see, for example, ref. 48). However, use of 0.1M NaNO,(aq) solution did not effect elution of any of the polymer samples within the range of calibration volumes established using the PEO standards.…”
Section: Resultsmentioning
confidence: 99%
“…Polyelectrolyte-gel adsorption can be reduced, in general, by addition of a low molar mass electrolyte to the eluant (see, for example, ref. 48). However, use of 0.1M NaNO,(aq) solution did not effect elution of any of the polymer samples within the range of calibration volumes established using the PEO standards.…”
Section: Resultsmentioning
confidence: 99%
“…Ils sont dus aux nombreux groupements hydroxyles, mais aussi à une petite quantité de groupements carboxyles présents sur le gel. Ces phéno-mènes peuvent être réduits en employant des éluants capables de supprimer ou de masquer l'ionisation des groupements parasites ; des solutions salines ont souvent été proposées ainsi que de l'eau acidifiée (KATO ef al., 1980 ;KATO, 1989). Les contraintes du DEDL nécessitant l'utilisation d'éluants évaporables nous ont amené à choisir l'eau acidifiée par de l'acide acétique.…”
Section: Les Conditions Chromatographiques Les éLuantsunclassified
“…Nous nous sommes donc fixés comme objectif d'améliorer le fractionnement en utilisant la chromatographie d'exclusion stérique haute performance après une pré-séparation par ultrafiltration ; pour cela, nous avons utilisé des données sur la séparation des substances humiques (DE HAAN ef al., 1978 ;DUBIN, 1988 ;HONGVE ef al., 1996 ;MATSUBARA ef al., 1994 ;POSNER, 1963) et plus générale-ment sur les macromolécules d'origine naturelle (HEJZLAR, 1987 ;KATO ef al., 1980 ;KATO, 1989 ;RINAUDO ef al., 1991). Expérimental protocol for ultrafiltration.…”
Section: Introductionunclassified
“…The solute-support interactions are of course undesirable for the study of the molecular weight distribution of the molecules but they can be made profitable for the optimisation of their separation. These interactions were previously described [4,6,7] and it was shown that the choice of the eluent is particularly critical in aqueous size exclusion chromatography. It is not trivial to eliminate such interactions but they can be, at least, strongly reduced by judiciously choosing the eluent properties (pH, ionic strength, addition of organic co-solvent...) [7].…”
mentioning
confidence: 91%
“…These interactions were previously described [4,6,7] and it was shown that the choice of the eluent is particularly critical in aqueous size exclusion chromatography. It is not trivial to eliminate such interactions but they can be, at least, strongly reduced by judiciously choosing the eluent properties (pH, ionic strength, addition of organic co-solvent...) [7]. Sodium nitrate is often preferred for the elution of humic substances in HPSEC.…”
mentioning
confidence: 91%