Aqueous Extracts of Wild Mushrooms Show Antimicrobial and Antiadhesion Activities against Bacteria and Fungi

Abstract

Mushrooms represent promising sources of novel bioactive compounds and can be applied as innovative strategies to control microbial contamination and infection via the food chain. We characterized aqueous extracts from 21 wild basidiomycete mushrooms and the cultivated oyster mushroom, Pleurotus ostreatus, as putative sources of antimicrobial and antiadhesive compounds. Broth microdilutions and adhesion to a polystyrene surface were evaluated on Gram-positive and Gram-negative bacteria and on fungi. The aqueous extracts tested showed antimicrobial and antiadhesive activities against these microorganisms. Biochemical analyses of the P. ostreatus extract indicated the involvement of several compounds with different molecular masses. Copyright © 2017 John Wiley & Sons, Ltd.

Anti-adhesion activity of phytochemicals to prevent Campylobacter jejuni biofilm formation on abiotic surfaces

“…4. Extracts prepared from the fruiting bodies of 21 wild basidiomycete mushrooms and cultivated oyster mushroom (Pleurotus ostreatus) have shown different anti-adhesion effects against C. jejuni on polystyrene surfaces (Klančnik et al 2017a). The highest activities were seen for Sparassis crispa and P. ostreatus aqueous extracts, with * 30% and 27% inhibition, respectively, although no dose dependency was seen for the comparison of 1 lg/mL and 10 lg/mL extract concentrations.…”

“…Biofilm formation is a multifactorial process that comprises different stages that are governed by different physical and chemical processes, and these all begin with the initial attachment. Adhesion of C. jejuni to abiotic surfaces is mediated by the following (Sulaeman et al 2012;Indikova et al 2015;Bohinc et al 2014;Š ikić Pogačar et al 2016;Trošt et al 2016;Klančnik et al 2017aKlančnik et al , b, 2018a):…”

“…For this review we used C. jejuni NCTC 11168 wild-type and its efflux pump mutants that lack the functional genes for expression of the efflux pump proteins CmeB, CmeR, CmeF (Klančnik et al 2012), Cj1687 and CmeG, with the aim being able to determine the differences in the adhesion properties to stainless steel. The reference and mutant strains were prepared as previously described (Klančnik et al 2012;Kovač et al 2015), and C. jejuni adherence to stainless steel was evaluated by cell cultivability (Trošt et al 2016;Klančnik et al 2017a), with a 24-h incubation in Mueller-Hinton broth at 42°C under microaerobic conditions. This was determined after washing and ultrasound treatment of the discs, and the data are presented as the numbers of C. jejuni cells (log CFU/mL).…”

Bioactive Phytochemical Constituents of Wild Edible Mushrooms from Southeast Asia

“…L. pyrifonne has also been reported to contain linoleic, oleic, palmitic, stearic, 9-eicosenoic, 9,12-eicosadienoic, tricosanoic, pentacosanoic, hexacosanoic, and 11-hexacosenoic acids [156]. Biological studies were conducted on the antioxidant and antimicrobial activities of L. pyrifonne [159][160][161]. Figure 12) were active against the plant parasitic nematode Meloidogyne incognita, and showed weak antimicrobial effects against Nadsonia fulvescens and Penicillium notatum.…”

“…(Agaricaceae) Extract [161] Compounds 63 and 64 [158]. Extract [160] Compound 65 [158] Compounds 63 and 64 (nematicidal activity [158]) [167]). Polysaccharide (immunomodulating activity [169]).…”