Aqueous Extracts of Lippia turbinata and Aloysia citriodora (Verbenaceae): Assessment of Antioxidant Capacity and DNA damage

Abstract

The aim of the present work was to make a contribution to the knowledge of aqueous extracts of Lippia turbinata and Aloysia citriodora (Verbenaceae; infusion and decoction) in relation with the establishment of its antioxidant activity and lack of DNA damage, for its potential use in therapeutics. The cytogenotoxic profile was evaluated through genotoxic biomarkers such as mitotic index, cellular proliferation kinetics, sister chromatid exchanges, single-cell gel electrophoresis assay, and micronucleus test in human peripheral blood lymphocyte cultures. No statistical differences were found (P > .05) between control and exposed cultures, even between both aqueous extracts. The total antioxidant capacity was shown to be higher in the decoction than in the infusion and both aqueous extracts protected against protein carbonylation and lipid peroxidation, the decoction being more efficient than the infusion (P < .005). These results suggest the safe use of these medicinal plants as chemoecologic agents in therapeutics.

Acute, Subacute, and Cell Toxicity of the Aqueous Extract of Lippia citriodora

“…Various studies have noted the potential risks involved with such plants (19). L. citriodora is one medicinal herb with a broad spectrum of therapeutic potential that needs to be evaluated for possible toxic effects (20).…”

Investigation of Aloysia citriodora Palau Samples in the Market in Terms of Antioxidant Capacity and Microbiological Contaminatio

“…Yapraklarından hazırlanan dekoksiyon etkin radikal süpürücü kapasite göstermezken, metanol ekstresi yüksek konsantrasyonda (1000 µg/mL) aktif bulunmuş-tur (25) . Yapılan in vitro antioksidan aktivite deneylerinde [TRAP (Toplam peroksil radikal yakalama antioksidan paremetresi), ABTS (2,2'-Azinobis(3-etilbenzotiazolin-6-sülfonat) ve DPPH] dekoksiyonu, infüzyonuna göre daha yüksek kapasite göstermiş ve yüksek total fenol içeriğine sahip olduğu belirtilmiştir (26) . Yapraklarından aseton, etanol, metanol ve su ile hazırlanan ekstrelerin total fenol içeriği belirlenmiş, Fe (III) iyonu indirgeyici antioksidan güç, DPPH radikalini süpürücü kapasite, toplam antioksidan kapasite deneyleri yapılmıştır.…”

“…Bitkininin çeşitli kısımları üzerinde de uçucu yağ analizi çalışmaları yapılmıştır (8,(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23) . Bitki özellikle in vitro olarak antioksidan kapasite (10,16,(24)(25)(26)(27)(28) , antimikrobiyal (16,25,29) , sitotoksik (25,26) ve antispazmodik (30,31) etkileri açı-sından incelenmiştir. İn vivo çalışmalar ise antienflamatuvar (6,30) , gastroprotektif (32) , antinosiseptif (11) , antipiretik, analjezik ve antioksidan kapasite (6) üzerinde yoğunlaşmıştır.…”

Medicinal plants for the "nerves": a review of ethnobotanical studies carried out in South Brazil

“…The aqueous extract of the leaves presented antispasmodic effect (tested in rat duodenum, in vitro) (Ragone et al 2007); sedative and anxiolytic effects in rats and mice (in vivo and in vitro) (Ragone et al 2010); antioxidant activity (Zamorano-Ponce et al 2006;Portmann et al 2012) and anti-inflammatory (in rats, in vitro and in vivo) (Ponce-Monter et al 2010). The hexane extract from the leaves presented a spasmolytic and anti-inflammatory effect in fragments of the uterus of female rats (Ponce-Monter et al 2010).…”