Aqueous Extract of Whitmania Pigra Whitman Alleviates Thrombus Burden Via Sirtuin 1/NF-κB Pathway

Yao, Xiaolan; Liu, Han; Li, Peng; Chen, Wen-pei; Guan, Shixia; Yang, Chen; Lin, Baoqin

doi:10.1016/j.jss.2019.07.094

Cited by 18 publications

(6 citation statements)

References 37 publications

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“…Recent studies suggest that in mouse models of aging, treatments that boost NAD + biosynthesis (e.g., administration of nicotinamide mononucleotide, a key NAD + precursor [ 246 ]) can activate sirtuins and reverse aging-induced endothelial dysfunction in the arterial system [ 23 , 34 ]. Initial evidence suggests that interventions that activate SIRT1 or SIRT3 likely exert beneficial effect on the aged venous system as well [ 249 , 250 ].…”

Section: Shared Mechanisms Of Vascular Aging Affecting the Venous Systemmentioning

confidence: 99%

The aging venous system: from varicosities to vascular cognitive impairment

et al. 2021

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Aging-induced pathological alterations of the circulatory system play a critical role in morbidity and mortality of older adults. While the importance of cellular and molecular mechanisms of arterial aging for increased cardiovascular risk in older adults is increasingly appreciated, aging processes of veins are much less studied and understood than those of arteries. In this review, age-related cellular and morphological alterations in the venous system are presented. Similarities and dissimilarities between arterial and venous aging are highlighted, and shared molecular mechanisms of arterial and venous aging are considered. The pathogenesis of venous diseases affecting older adults, including varicose veins, chronic venous insufficiency, and deep vein thrombosis, is discussed, and the potential contribution of venous pathologies to the onset of vascular cognitive impairment and neurodegenerative diseases is emphasized. It is our hope that a greater appreciation of the cellular and molecular processes of vascular aging will stimulate further investigation into strategies aimed at preventing or retarding age-related venous pathologies.

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Section: Shared Mechanisms Of Vascular Aging Affecting the Venous Systemmentioning

confidence: 99%

The aging venous system: from varicosities to vascular cognitive impairment

et al. 2021

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“…Since, Hirudo nipponica Whitman (Leech), and Pheretima aspergillum (E. Perrier) (earthworm) are animals, they mainly consists of proteins, peptides, amino acids, fatty acids, phospholipids, mineral substances, nucleosides and other compounds, therefore, out of scope in the currently used chromatographic conditions. Besides various therapeutic effects exerted via multiple signalling pathways, all these five Chinese medicines posses one common anti-inflammatory property through inactivation of NFκB signalling pathway ( Zhao et al, 2015 ; Huang et al, 2016 ; Li et al, 2016 ; Lou et al, 2019 ; Yao et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Zhilong Huoxue Tongyu Capsules Ameliorate Early Brain Inflammatory Injury Induced by Intracerebral Hemorrhage via Inhibition of Canonical NFкβ Signalling Pathway

et al. 2022

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Background: Intracerebral hemorrhage (ICH) is a debilitating and fatal condition with continuously rising incidence globally, without effective treatment available. Zhilong Huoxue Tongyu (ZLHXTY) capsule is a traditional Chinese medicine that is used for ICH treatment in China. However, the evidence based mechanism is not clear.Purpose: To study the protective effects of ZLHXTY capsules against ICH pathogenesis via targetting nuclear factor kappa β (NFкβ) canonical signalling pathway.Methods: C57BL/6 J mice ICH models using autologous blood injection were used to study the effect of ZLHXTY (1.4 g/kg P.O.) after 24 and 72 hrs of ICH induction. The neurological scoring, corner turn test and balance beam with scoring was performed to assess neurological damage. Hematoxylin/eosin and nissl staining was used for histopathological evaluation. Levels of TNFα, NFкB, iNOS, COX2, IL1, IL6 were measured using real time qPCR and western blotting. Protein levels of IKKβ and IкBα were analyzed through western blotting. Immunofluorescence for co-expression of NeuN/TNFα, NeuN/NFкB, Iba1/TNFα, and Iba1/NFкB was also performed.Results: Treatment with ZLHXTY capsules after ICH ameliorated inflammatory brain injury after 24 and 72 h; revealed by neurological scoring, hematoxylin/eosin and nissl staining. The qPCR and western blot analyses demonstrated significant downregulation of TNFα, NFкB, iNOS, COX2, IL1β and IL6. Further, the IKKβ and IкBα revealed significant downregulation and upregulation respectively in western blot. Immunofluorescence also revealed attenuated expression of TNFα and NFкB in neurons and also low expression of Iba1.Conclusion: ZLHXTY capsules elicit its neuroprotective effect by targetting the NFкβ canonical signalling pathway, thereby ameliorating the ICH induced brain injury.

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“…Sirtuins are a competent therapeutic molecule proven protective in oxidative and ER stress-induced pathways such as inflammation and apoptosis (Hotamisligil, 2010;Tabas, 2010). Aqueous extract of Whitmania Pigra, a traditional Chinese medicine to treat cardiovascular diseases, has demonstrated to reduce deep vein thrombosis burden by inhibiting inflammation via SIRT1/NF-κB signaling pathway (Yao et al, 2020). Potent pharmacological Sirtuins activators like Sirtuins activating compounds (STAC) that increase the Sirtuins activity are available and few of them are currently under clinical trials in various diseases.…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Therapeutic Role of Sirtuins Targeting Unfolded Protein Response, Coagulation, and Inflammation in Hypoxia-Induced Thrombosis

2021

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Thrombosis remains one of the leading causes of morbidity and mortality across the world. Many pathological milieus in the body resulting from multiple risk factors escort thrombosis. Hypoxic condition is one such risk factor that disturbs the integrity of endothelial cells to cause an imbalance between anticoagulant and procoagulant proteins. Hypoxia generates reactive oxygen species (ROS) and triggers inflammatory pathways to augment the coagulation cascade. Hypoxia in cells also activates unfolded protein response (UPR) signaling pathways in the endoplasmic reticulum (ER), which tries to restore ER homeostasis and function. But the sustained UPR linked with inflammation, generation of ROS and apoptosis stimulates the severity of thrombosis in the body. Sirtuins, a group of seven proteins, play a vast role in bringing down inflammation, oxidative and ER stress and apoptosis. As a result, sirtuins might provide a therapeutic approach towards the treatment or prevention of hypoxia-induced thrombosis. Sirtuins modulate hypoxia-inducible factors (HIFs) and counteract ER stress-induced apoptosis by attenuating protein kinase RNA-like endoplasmic reticulum kinase (PERK)/Eukaryotic translation initiation factor 2α (eIF2α) pathway activation. It prevents ER-stress mediated inflammation by targeting X-Box Binding Protein 1 (XBP1) and inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κβ) signaling through deacetylation. Sirtuins also obstruct nucleotide-binding domain, leucine-rich-containing family, pyrin domain containing 3 (NLRP3) inflammasome activation to reduce the expression of several pro-inflammatory molecules. It protects cells against oxidative stress by targeting nuclear factor erythroid 2-related factor 2 (Nrf2), glutathione (GSH), forkhead box O3 (FOXO3), superoxide dismutase (SOD), catalase (CAT), peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α), glucose-6-phosphate dehydrogenase (G6PD), phosphoglucomutase-2 (PGAM2), and NF-κB, to name few. This review, thus, discusses the potential role of sirtuins as a new treatment for hypoxia-induced thrombosis that involves an intersection of UPR and inflammatory pathways in its pathological manifestation.

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Aqueous Extract of Whitmania Pigra Whitman Alleviates Thrombus Burden Via Sirtuin 1/NF-κB Pathway

Cited by 18 publications

References 37 publications

The aging venous system: from varicosities to vascular cognitive impairment

The aging venous system: from varicosities to vascular cognitive impairment

Zhilong Huoxue Tongyu Capsules Ameliorate Early Brain Inflammatory Injury Induced by Intracerebral Hemorrhage via Inhibition of Canonical NFкβ Signalling Pathway

Therapeutic Role of Sirtuins Targeting Unfolded Protein Response, Coagulation, and Inflammation in Hypoxia-Induced Thrombosis

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