2017
DOI: 10.21767/2469-2980.100043
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Aqueous Extract of Psidium cattleianum as Intracanal Medication: An In Vitro Study of Cell Viability

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Cited by 2 publications
(1 citation statement)
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“…Traditionally, the extraction of polyphenols from PC leaves was performed using conventional methods (stirring, pressing, maceration, and hydrodistillation) with or without thermal treatments [ 13 , 14 ]. Different authors used decoction (100 g of dried leaves/600 mL deionized water at 100 °C for 5 min) [ 15 , 16 , 17 ], Soxhlet or heat reflux extraction (2 g of dried leaves/100 mL of hexane at 90 °C for 90 min) [ 18 ], hydro-distillation (for 2 to 5 h in a modified Clevenger apparatus) [ 19 , 20 ], stirring at room temperature (1.2 kg of dried leaves/12 L of 80% methanol for 3 days) [ 21 , 22 ], hand-shaking (20 g of dried leaves/250 mL of 80% ethanol, shaking vigorously 5 times a day for 12 days) [ 23 ], and maceration at room temperature with methanol (1.2 kg of dried leaves/12 L of 80% methanol for 14 days) [ 24 ] or ethanol (0.5 kg of dried leaves/500 mL for 5 days) [ 11 ] to extract bioactive compounds from PC leaves. However, these methods required high amounts of solvents and samples, and long extraction times that may affect the stability (oxidation, ionization, and hydrolysis), yield, and bioactivity of these phytochemicals [ 13 , 14 ].…”
Section: Introductionmentioning
confidence: 99%
“…Traditionally, the extraction of polyphenols from PC leaves was performed using conventional methods (stirring, pressing, maceration, and hydrodistillation) with or without thermal treatments [ 13 , 14 ]. Different authors used decoction (100 g of dried leaves/600 mL deionized water at 100 °C for 5 min) [ 15 , 16 , 17 ], Soxhlet or heat reflux extraction (2 g of dried leaves/100 mL of hexane at 90 °C for 90 min) [ 18 ], hydro-distillation (for 2 to 5 h in a modified Clevenger apparatus) [ 19 , 20 ], stirring at room temperature (1.2 kg of dried leaves/12 L of 80% methanol for 3 days) [ 21 , 22 ], hand-shaking (20 g of dried leaves/250 mL of 80% ethanol, shaking vigorously 5 times a day for 12 days) [ 23 ], and maceration at room temperature with methanol (1.2 kg of dried leaves/12 L of 80% methanol for 14 days) [ 24 ] or ethanol (0.5 kg of dried leaves/500 mL for 5 days) [ 11 ] to extract bioactive compounds from PC leaves. However, these methods required high amounts of solvents and samples, and long extraction times that may affect the stability (oxidation, ionization, and hydrolysis), yield, and bioactivity of these phytochemicals [ 13 , 14 ].…”
Section: Introductionmentioning
confidence: 99%