Aqueous extract ofLiriope platyphylla, a traditional Chinese medicine, significantly inhibits abdominal fat accumulation and improves glucose regulation in OLETF type II diabetes model rats

Abstract

Liriope platyphylla is a medical herb that has long been used in Korea and China to treat cough, sputum, neurodegenerative disorders, obesity, and diabetes. The aims of this study were to determine the antidiabetic and antiobesity effects of aqueous extract of L. platyphylla (AEtLP) through glucose and lipid regulation in both pre-diabetes and obesity stage of type II diabetes model. Two concentrations of AEtLP were orally administrated to OLETF (Otsuka Long-Evans Tokushima Fatty) rats once a day for 2 weeks, after which changes in glucose metabolism and fat accumulation were measured. Abdominal fat mass dramatically decreased in AEtLP-treated OLETF rats, whereas glucose concentration slightly decreased in all AEtLP-treated rats. However, compared to vehicle-treated OLETF rats, only AEtLP10 (10% concentration)-treated OLETF rats displayed significant induction of insulin production, whereas AEtLP5 (5% concentration)-treated OLETF rats showed a lower level of insulin. Although serum adiponectin level increased in only AEtLP5-treated rats, significant alteration of lipid concentration was detected in AEtLP5-treated OLETF rats. Expression of Glut-1 decreased in all AEtLP-treated rats, whereas Akt phosphorylation increased only in AEtLP10-treated OLETF rats. Furthermore, the pattern of Glut-3 expression was very similar with that of Glut-1 expression, which roughly corresponded with the phosphorylation of c-Jun N-teminal kinase (JNK) and p38 in the mitogen-activated protein kinase pathway. Therefore, these findings suggest that AEtLP should be considered as a therapeutic candidate during pre-diabetes and obesity stage capable of inducing insulin secretion from pancreatic β-cells, glucose uptake in liver cells, as well as a decrease in fat and lipid accumulation.

Phytochemical and pharmacological overview on <i>Liriopes radix</i>

“…AEtLP to ICR mice displayed a significant reduction of the glucose and increased insulin concentrations [37]. Significant alterations on serum adiponectin and lipid concentration [35,36] with GLUT-3, GLUT-1, JNK, and p38 pathway [35] and SOD expressions [37] were also reported. The insulin level was dramatically elevated upon co-treatment with high glucose and AEtLP in INS-1 cells may be tightly correlated with calcium regulation [38,39].…”

“…In addition, it inhibits glycogen synthase kinase-3 (GSK-3) expression and increases IR, IRS-1, PI3K, protein kinase B (Akt), and glucose transport protein (GLUT)-4 expressions in the liver [34]. AEtLP dramatically decreased the abdominal fat mass and slightly decreased the glucose concentration [35], although no significant differences in body weight, glucose tolerance, and glucose concentration [36] in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. AEtLP to ICR mice displayed a significant reduction of the glucose and increased insulin concentrations [37].…”

Laxative effects ofLiriope platyphyllaare tightly correlated with suppression of endoplasmic reticulum stress in loperamide-induced constipation of SD rats

“…Next, 600 g of dry roots were reduced to powder using an electric blender, then extracted in 2 L of distilled water, after which the water extract was purified at 100℃ for 2 h using circulating extraction equipment (IKA Labortechnik, Staufen, Germany). The calories and main composition of AEtLP were measured in previous studies [ 15 16 ]. Extract solutions were concentrated into dry pellets using a rotary evaporator (EYELA, Tokyo, Japan), then stored at -80℃ until use.…”

The complete chloroplast genome sequence of a medicinal herb Liriope Platyphylla (Asparagaceae)

“…A root tuber of L. platyphylla has been proven the efficacy of anti-inflammatory, anti-asthmatic effects (Lee et al 2005). It is also reported that aqueous extract of L. platyphylla has been shown to be effective in type 2 diabetes mellitus (Kim et al 2012). These effects come from many bioactive substances contained in their tuber.…”