Aqueous chromatography system using pH- and temperature-responsive stationary phase with ion-exchange groups

Ayano, Eri; Nambu, Kyoko; Sakamoto, Chiaki; Kanazawa, Hideko; Kikuchi, Akihiko; Okano, Teruo

doi:10.1016/j.chroma.2006.01.068

Cited by 57 publications

(37 citation statements)

References 24 publications

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“…This has already been illustrated in the pioneering work on TR-LC by the group of Okana et al [4][5][6] and in many applications including the analysis of small molecules, pharmaceuticals and biomolecules [7,8]. Columns containing copolymers of NIPAAm with hydrophobic [5][6][7]9], ionic [8,10], and affinity ligands [11] have extended the applicability range of TR-LC.…”

Section: Introductionmentioning

confidence: 89%

Thermoresponsive Poly(N-vinylcaprolactam) as Stationary Phase for Aqueous and Green Liquid Chromatography

Miserez

Lynen

Wright

et al. 2009

Chroma

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Section: Introductionmentioning

confidence: 89%

Thermoresponsive Poly(N-vinylcaprolactam) as Stationary Phase for Aqueous and Green Liquid Chromatography

Miserez

Lynen

Wright

et al. 2009

Chroma

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“…While the HCO elution peak remained unchanged, the retention time of TES decreased from 30.9 to 19.9 min and the elution peak became sharper. In conventional chromatography, elution is modulated by the addition of organic solvents or increasing the salt concentration in the mobile phase, or by heating/cooling the entire mobile phase [40][41][42]. The proposed AMF system instead relies on the self-heating of the stationary phase, which is controlled by switching the AMF on and off.…”

Section: Amf-induced Switchable Elution Control Of Steroidsmentioning

confidence: 99%

“…Because this type of ionic copolymer is known to shift its pKa in response to temperature changes, the silica beads exhibited simultaneous changes in wettability and charge density under thermal stimuli. These systems can be cost-effective and environmentally friendly, because the elution profiles can be controlled using only an aqueous solution as a mobile phase; however, their performance is adversely affected by the heating/cooling process of the entire mobile phase [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Surface design with self-heating smart polymers for on–off switchable traps

Techawanitchai

Yamamoto

Ebara

et al. 2011

Science and Technology of Advanced Materials

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We have developed a novel self-heating, temperature-responsive chromatography system for the effective separation of biomolecules. Temperature-responsive poly(N-isopropylacrylamide-co-N-hydroxymethylacrylamide), poly(NIPAAm-co-HMAAm), was covalently grafted onto the surface of magnetite/silica composites as 'on-off' switchable surface traps. The lower critical solution temperature (LCST) of the poly(NIPAAm-co-HMAAm)s was controlled from 35 to 55• C by varying the HMAAm content. Using the heat generated by magnetic particles in an alternating magnetic field (AMF) we were able to induce the hydrophilic to hydrophobic phase separation of the grafted temperature-responsive polymers. To assess the feasibility of the poly(NIPAAm-co-HMAAm)-grafted magnetite/silica particles as the stationary phase for chromatography, we packed the particles into the glass column of a liquid chromatography system and analyzed the elusion profiles for steroids. The retention time for hydrophobic steroids markedly increased in the AMF, because the hydrophobic interaction was enhanced via self-heating of the grafted magnetite/silica particles, and this effect could be controlled by changing the AMF irradiation time. Turning off the AMF shortened the total analysis time for steroids. The proposed system is useful for separating bioactive compounds because their elution profiles can be easily controlled by an AMF.

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“…In 2006, they developed a new HPLC method for the analysis of non-steroidal antiinflammatory drugs such as ibuprofen and ketoprofen (6). They designed a new temperature/pH sensitive stationary phase by modifying aminopropyl silica beads with a NIPAM-based nanoparticle with two incorporated co-monomers: butyl methacrylate (BMA) and N,Ndimethylaminopropylacrylamide (DMAPAAm).…”

Section: Introductionmentioning

confidence: 99%

“…The latter can be used in many applications such as the control of cell and protein adhesion, (1-4) and bioseparation (5,6).…”

Section: Introductionmentioning

confidence: 99%

Deposition of fluorescent NIPAM-based nanoparticles on solid surfaces: Quantitative analysis and the factors affecting it

Mohsen

Thorne

Alexander

et al. 2014

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Abstract Recently, responsive surfaces have attracted attention due to their potential applications. Reported research have studied the deposition of environmentally responsive particles on different surfaces, qualitatively tested their response to environmental conditions and studied their possible applications. In this work, novel fluorescent temperature-sensitive nanoparticles were synthesized using a surfactant free emulsion polymerization technique: poly(N-isopropylacrylamide-co-5% vinyl cinnamate) (p(NIPAM)5%VC). The new particles were characterized using dynamic light scattering and fluorescence spectroscopy. A novel sensitive method for the quantitative analysis of p(NIPAM) 5% VC using fluorescence spectroscopy was developed to determine the concentration of nanoparticle dispersions. This was further used to quantitatively determine the mass of nanoparticles deposited per unit area of glass pre-treated with acid, glass pre-treated with base, quartz, stainless steel, gold and teflon at 25°C and 60°C. Factors affecting the adsorption/desorption of the nanoparticles were studied, including the effect of substrate surface charge, surface roughness (using atomic force microscopy, AFM), hydrophilicity/hydrophobicity and the temperature at which the adsorption/desorption experiments were carried out. The results show that the effect of surface charge is the most significant, followed by that of surface roughness and temperature. Meanwhile, the influence of the hydrophobicity/hydrophilicity of the surface on the adsorption/desorption of nanoparticles appears to be far less significant than the previously mentioned factors.Keywords: fluorescent p(NIPAM), vinyl cinnamate, quantitative particle deposition, surface charge and surface roughness.

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Aqueous chromatography system using pH- and temperature-responsive stationary phase with ion-exchange groups

Cited by 57 publications

References 24 publications

Thermoresponsive Poly(N-vinylcaprolactam) as Stationary Phase for Aqueous and Green Liquid Chromatography

Thermoresponsive Poly(N-vinylcaprolactam) as Stationary Phase for Aqueous and Green Liquid Chromatography

Surface design with self-heating smart polymers for on–off switchable traps

Deposition of fluorescent NIPAM-based nanoparticles on solid surfaces: Quantitative analysis and the factors affecting it

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