Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase

Kanazawa, Hideko; Nishikawa, Mayumi; Mizutani, Aya; Sakamoto, Chiaki; Morita-Murase, Yuko; Nagata, Yoshiko; Kikuchi, Akihiko; Okano, Teruo

doi:10.1016/j.chroma.2008.01.056

Cited by 59 publications

(46 citation statements)

References 16 publications

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“…The temperature-dependent protein sorption behaviour of thermoresponsive ion exchange adsorbents described in this and other work [19][20][21][22][23][24][25]46] makes them attractive prospects for regenerate-free isocratic liquid chromatography, potentially providing viable solutions to issues such as sub-optimal elution/cleaning performance and excessive buffer consumption/waste generation. However, with very few exceptions [47], all reported investigations on thermoresponsive liquid chromatography use conventional fixed-bed columns filled with thermoresponsive adsorbents, and the whole column is tempered by a water bath, a water jacket connected to a water bath, or a column oven [16][17][18][19]24,26].…”

Section: Chromatography Of Lf In Jacketed Columns Containing Thermocementioning

confidence: 99%

“…Most of this has concentrated on exploiting the principle of thermally induced extension and collapse of polymer chains for modulating the fractionation range in size exclusion chromatography [11,12,14], hydrophobicity in HPLC [13,[15][16][17][18], and balancing electrostatic and hydrophobic interactions in ion exchange chromatography, through the use of copolymers containing both thermoresponsive and ion exchange components [19][20][21][22][23][24][25], rather than mitigating ligand masking and 'forced elution' in pseudoaffinity chromatography [3,26]. To this day, the vast body of work on thermoresponsive chromatography pertains to the modification and subsequent use of small pored inorganic (glass, silica) or hydrophobic (polystyrene based) chromatography supports in analytical HPLC separations of small biomolecules (especially steroids).…”

Section: Introductionmentioning

confidence: 99%

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Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement

Müller

Cao

Ewert

et al. 2013

Journal of Chromatography A

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Section: Chromatography Of Lf In Jacketed Columns Containing Thermocementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement

Müller

Cao

Ewert

et al. 2013

Journal of Chromatography A

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“…The latter can be used in many applications such as the control of cell and protein adhesion, (1-4) and bioseparation (5,6).…”

Section: Introductionmentioning

confidence: 99%

Deposition of fluorescent NIPAM-based nanoparticles on solid surfaces: Quantitative analysis and the factors affecting it

Mohsen

Thorne

Alexander

et al. 2014

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Abstract Recently, responsive surfaces have attracted attention due to their potential applications. Reported research have studied the deposition of environmentally responsive particles on different surfaces, qualitatively tested their response to environmental conditions and studied their possible applications. In this work, novel fluorescent temperature-sensitive nanoparticles were synthesized using a surfactant free emulsion polymerization technique: poly(N-isopropylacrylamide-co-5% vinyl cinnamate) (p(NIPAM)5%VC). The new particles were characterized using dynamic light scattering and fluorescence spectroscopy. A novel sensitive method for the quantitative analysis of p(NIPAM) 5% VC using fluorescence spectroscopy was developed to determine the concentration of nanoparticle dispersions. This was further used to quantitatively determine the mass of nanoparticles deposited per unit area of glass pre-treated with acid, glass pre-treated with base, quartz, stainless steel, gold and teflon at 25°C and 60°C. Factors affecting the adsorption/desorption of the nanoparticles were studied, including the effect of substrate surface charge, surface roughness (using atomic force microscopy, AFM), hydrophilicity/hydrophobicity and the temperature at which the adsorption/desorption experiments were carried out. The results show that the effect of surface charge is the most significant, followed by that of surface roughness and temperature. Meanwhile, the influence of the hydrophobicity/hydrophilicity of the surface on the adsorption/desorption of nanoparticles appears to be far less significant than the previously mentioned factors.Keywords: fluorescent p(NIPAM), vinyl cinnamate, quantitative particle deposition, surface charge and surface roughness.

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“…We have been investigating temperature-responsive chromatography, in which a thermo-responsive polymer such as poly(N-isopropylacrylamide) (PNIPAAm) is used as the stationary phase [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. PNIPAAm exhibits thermally reversible solubility changes in aqueous solutions in response to temperature changes across its lower critical solution temperature (LCST).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Psychoactive Drugs by Temperature-Responsive Chromatography

et al. 2017

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Temperature-responsive chromatography, in which the characteristics of the stationary phase can be controlled by varying the column temperature with only an aqueous eluent, was applied to the analysis of psychoactive drugs. Temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm)-based copolymers were synthesized with n-butyl methacrylate (BMA) and N-acryloyl L-phenylalanine methyl ester (Phe-OMe) as a comonomer. These polymers were grafted onto aminopropyl silica and used as the stationary phase. Seven psychoactive drugs could be separated simultaneously by the column, including BMA. Among the seven drugs, for triazolam, which has a triazole ring in its structure, the order of elution changed on changing the column temperature. The phenomenon could be explained by NH-π interactions between the NH groups of PNIPAAm and π electrons of the triazole ring. The conformational changes of PNIPAAm altered the degree of exposure of the NH groups and would affect the elution order of the analytes. To enhance the molecular recognition ability for the triazole ring, the column containing Phe-OMe was used. Noticeable changes in the retention factors occurred due to the additional π-π interactions between the phenyl moieties of Phe-OMe and the triazole ring. The effects of the π-π interactions were also altered by the changes in column temperature. This chromatographic system, which needs no toxic organic solvent, offers an effective way to determine the causes of addiction in medical institutions.

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Aqueous chromatographic system for separation of biomolecules using thermoresponsive polymer modified stationary phase

Cited by 59 publications

References 16 publications

Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement

Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement

Deposition of fluorescent NIPAM-based nanoparticles on solid surfaces: Quantitative analysis and the factors affecting it

Analysis of Psychoactive Drugs by Temperature-Responsive Chromatography

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