Aqueous and gaseous plasma applications for the treatment of mung bean seeds

Darmanin, Martina; Fröhling, Antje; Bußler, Sara; Durek, Julia; Neugart, Susanne; Schreiner, Monika; Blundell, Renald; Gatt, Ruben; Schlüter, Oliver; Valdramidis, Vasilis P.

doi:10.1038/s41598-021-97823-1

Cited by 11 publications

(10 citation statements)

References 49 publications

(63 reference statements)

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“…The germination percentage reached up to 100% (from 94%) when plasma and PAW (CO 2 ) were used; however, the PAW generated using O 2 [PAW(O 2 )] inactivated more microorganisms than the other PAW. 79 Apart from germination enhancement, plasma also has the ability to change the minerals, pigments, enzymes, and other nutritional compositions of the seeds. For example, in capacitively coupled plasma (CCP) treated tomato seeds, calcium (7.73%) and boron (11.53%) contents were increased; 80 whereas in soybean, nutrient fractions such as soluble protein and soluble sugar and enzymes such as peroxidase (POD) and phenylalanine ammonia-lyase (PAL) activities increased aer CDPJ treatment.…”

Section: Plasma Treatment Of Seeds/plantsmentioning

confidence: 99%

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Plasma processing: a sustainable technology in agri-food processing

Rajan

Boopathy

Radhakrishnan

et al. 2023

Sustainable Food Technol.

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Section: Plasma Treatment Of Seeds/plantsmentioning

confidence: 99%

Section: Plasma Applications In Pre-harvest Stagesmentioning

confidence: 99%

Plasma processing: a sustainable technology in agri-food processing

Rajan

Boopathy

Radhakrishnan

et al. 2023

Sustainable Food Technol.

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“…Sprout seeds are the primary contaminants for an enclosed sprout production line to contaminate the final sprout before packaging and distribution ( Darmanin et al, 2021 ). Sprout seeds require warm and humid conditions for germination ( NACMCF., 1999 ); macromolecules containing nutrient substances will be released during germination to facilitate the growth of surrounding bacteria ( Yang et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of polyvalent Escherichia phage Sa157lw for the biocontrol potential of Salmonella Typhimurium and Escherichia coli O157:H7 on contaminated mung bean seeds

Liao

Zhang²,

Salvador³

et al. 2022

Front. Microbiol.

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Seeds are one of the primary sources of contamination with foodborne pathogens, such as pathogenic Escherichia coli, and various Salmonella serovars, for produce, particularly sprouts. Due to the susceptibility of sprout growth to chemical-based antimicrobials and the rising issue of antimicrobial resistance, developing innovative antimicrobial interventions is an urgent need. Therefore, the objective of this study was to characterize Escherichia phage Sa157lw (or Sa157lw) for the biocontrol potential of Salmonella Typhimurium and E. coli O157:H7 on contaminated mung bean seeds. Phage Sa157lw was subjected to whole-genome sequencing and biological characterization, including morphology, one-step growth curve, and stress stability tests. Later, antimicrobial activity was determined in vitro and upon application on the mung bean seeds artificially contaminated with E. coli O157:H7 or Salmonella Typhimurium. Sa157lw possessed a contractile tail and belonged to the Kuttervirus genus under the Ackermannviridae family, sharing a close evolutionary relationship with E. coli phage ECML-4 and Kuttervirus ViI; however, tail spike genes (ORF_102 and ORF_104) were the primary region of difference. Comparative genomics showed that Sa157lw encoded a cluster of tail spike genes—including ORF_101, ORF_102, and ORF_104—sharing high amino acid similarity with the counterfeits of various Salmonella phages. Additionally, Sa157lw harbored a unique tail fiber (ORF_103), possibly related to the receptors binding of O157 strains. The genomic evidence accounted for the polyvalent effects of Sa157lw against E. coli O157:H7 and various Salmonella serovars (Typhimurium, Enteritidis, Agona, Saintpaul, and Heidelberg). Furthermore, the phage did not contain any virulence, antibiotic-resistant, or lysogenic genes. Sa157lw had a 30-min latent period on both E. coli O157:H7 and Salmonella Typhimurium, with an estimated burst size of 130 and 220 PFU/CFU, respectively, and was stable at a wide range of temperatures (4–60°C) and pH (pH4 to pH10). The phage application demonstrated a strong anti-E. coli O157:H7 and anti-Salmonella Typhimurium effects in 1.1 and 1.8 log reduction on the contaminated mung bean seeds after overnight storage at 22°C. These findings provide valuable insights into the polyvalent Sa157lw as a potential biocontrol agent of Salmonella Typhimurium and E. coli O157:H7 on sprout seeds.

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“…Their nutrients, especially isoflavone, and selenium biofortification, are notably improved due to germination [ 4 , 5 ]. However, soybean sprouts are particularly prone to microbial contamination due to their high nutrient content and the warm temperatures and humid conditions needed for their production [ 3 , 6 ]. Various studies have demonstrated that the germination step is the main source of microbial contamination, with 3.0–6.0 log CFU/g microbes in sprouts, which are 2 or 3 logs greater than those observed outside sprouts [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Detection and Degradation Characterization of 16 Quinolones in Soybean Sprouts by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

Deng

Feng

et al. 2022

Foods

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Recently, there have been increasing safety concerns about the illegal abuse of quinolone in soybean sprouts. This study aimed to establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous detection of 16 quinolones (QNs) in soybean sprouts, and then reveal their degradation characteristics. The samples were extracted with acetonitrile (with 1% formic acid), purified by a C18 adsorbent, and separated by an ACQUITY UPLC BEH C18 (1.7 μm, 2.1 mm × 100 mm) column. The internal standard method was applied for quantitative determination. The results demonstrated that the quantification linear range for 16 QNs was between 2.0 ng/mL and 50.0 ng/mL. The detection limits were between 0.5 μg/kg and 4.0 μg/kg, and the quantification limits were between 2.0 μg/kg and 20.0 μg/kg. This method was used to screen for quinolones in 50 batches of market soybean sprouts; the obtained results showed good agreement with those of the standard method. It was found that QNs possessed longer degradation half-life (T1/2) in the storage stage of soybean sprouts, while they degraded to some extent during the germination stage via active enzyme action. In particular, ciprofloxacin was the most stable QNs with a T1/2 of 70.71 d during the storage stage of soybean sprouts. This work not only offers an accurate and efficient QNs residual analysis strategy but also provides a reference for the supervision and management of QNs in foods.

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Aqueous and gaseous plasma applications for the treatment of mung bean seeds

Cited by 11 publications

References 49 publications

Plasma processing: a sustainable technology in agri-food processing

Plasma processing: a sustainable technology in agri-food processing

Characterization of polyvalent Escherichia phage Sa157lw for the biocontrol potential of Salmonella Typhimurium and Escherichia coli O157:H7 on contaminated mung bean seeds

Detection and Degradation Characterization of 16 Quinolones in Soybean Sprouts by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

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