Aquaporins 6-12 in the human eye

Tran, Thuy Linh; Bek, Toke; Holm, Lars; Cour, Morten la; Nielsén, Sören; Prause, Jan Ulrik; Rojek, Aleksandra; Hamann, Steffen; Heegaard, Steffen

doi:10.1111/j.1755-3768.2012.02547.x

Cited by 36 publications

(27 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lens epithelial cells contain AQP1 [15]. Recent immunohistochemistry studies also demonstrated AQP5 and AQP7 labeling in the lens epithelium; however, their relative abundance has not been measured [65–67]. In lens fiber cells, AQP0, formerly known as major intrinsic protein (MIP), is highly abundant comprising over 50% of lens membrane protein concentration.…”

Section: Lensmentioning

confidence: 99%

Aquaporins in the eye: Expression, function, and roles in ocular disease

Schey

Wang

Wenke

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

110

100

View full text Add to dashboard Cite

Background All thirteen known mammalian aquaporins have been detected in the eye. Moreover, aquaporins have been identified as playing essential roles in ocular functions ranging from maintenance of lens and corneal transparency to production of aqueous humor to maintenance of cellular homeostasis and regulation of signal transduction in the retina. Scope of review This review summarizes the expression and known functions of ocular aquaporins and discusses their known and potential roles in ocular diseases. Major conclusions Aquaporins play essential roles in all ocular tissues. Remarkably, not all aquaporin function as a water permeable channel and the functions of many aquaporins in ocular tissues remain unknown. Given their vital roles in maintaining ocular function and their roles in disease, aquaporins represent potential targets for future therapeutic development. General significance Since aquaporins play key roles in ocular physiology, an understanding of these functions is important to improving ocular health and treating diseases of the eye. It is likely that future therapies for ocular diseases will rely on modulation of aquaporin expression and/or function. This article is part of a Special Issue entitled Aquaporins.

show abstract

Section: Lensmentioning

confidence: 99%

Aquaporins in the eye: Expression, function, and roles in ocular disease

Schey

Wang

Wenke

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

110

100

View full text Add to dashboard Cite

show abstract

“…In these original reports, AQP9 mRNA expression was found in a few tissues, most notably liver and leukocytes in humans (Ishibashi et al 1998;Tsukaguchi et al 1999), as well as liver and immature sperm in mice (Tsukaguchi et al 1998). Since then, AQP9 immunoreactivity has been found in many tissues, including rat and human epididymis (Elkjaer et al 2000;Pastor-Soler et al 2001), various cell types of rodent and primate brain (Elkjaer et al 2000;Badaut et al 2001;Badaut et al 2004;Amiry-Moghaddam et al 2005;Arcienega et al 2010), mouse spinal cord (Oshio et al 2004), human chorioamnion and placenta (Damiano et al 2001;Wang et al 2004), mouse and human inner ear (Huang et al 2002;Degerman et al 2011), mouse and human small intestine (Okada et al 2003), rat and human prostate (Wang et al 2008;Hwang et al 2012), human skeletal muscle (Inoue et al 2009), urothelium (Rubenwolf et al 2009), rat and porcine oviduct (Skowronski et al 2009), human adipose tissue (Rodriguez et al 2011), human retina (Tran et al 2013;Yang et al 2013), and mouse and human skin (Sugiyama et al 2014). …”

Section: Introductionmentioning

confidence: 99%

A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues

Lindskog

Asplund

Catrina

et al. 2016

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

SummaryAQP9 is known to facilitate hepatocyte glycerol uptake. Murine AQP9 protein expression has been verified in liver, skin, epididymis, epidermis and neuronal cells using knockout mice. Further expression sites have been reported in humans. We aimed to verify AQP9 expression in a large set of human normal organs, different cancer types, rheumatoid arthritis synovial biopsies as well as in cell lines and primary cells. Combining standardized immunohistochemistry with high-throughput mRNA sequencing, we found that AQP9 expression in normal tissues was limited, with high membranous expression only in hepatocytes. In cancer tissues, AQP9 expression was mainly found in hepatocellular carcinomas, suggesting no general contribution of AQP9 to carcinogenesis. AQP9 expression in a subset of rheumatoid arthritis synovial tissue samples was affected by Humira, thereby supporting a suggested role of TNFα in AQP9 regulation in this disease. Among cell lines and primary cells, LP-1 myeloma cells expressed high levels of AQP9, whereas low expression was observed in a few other lymphoid cell lines. AQP9 mRNA and protein expression was absent in HepG2 hepatocellular carcinoma cells. Overall, AQP9 expression in human tissues appears to be more selective than in mice. (J Histochem Cytochem 64:287-300, 2016)

show abstract

“…7 However, more recently, human native RPE cells have been shown to express several other AQPs, including AQP0, AQP1, AQP3, AQP4, AQP5, AQP6, AQP7, AQP8, AQP9, AQP10, AQP11, and AQP12, but not AQP2. 8,9 In addition, in a microarray study AQP3 was shown to be expressed by human stem cell-derived mature RPE cells but not by an immortalized RPE cell line. 10 In a similar microarray survey mature stem cellderived RPE cells were shown to also express AQP1.…”

mentioning

confidence: 99%

Aquaporin Expression and Function in Human Pluripotent Stem Cell–Derived Retinal Pigmented Epithelial Cells

Juuti‐Uusitalo

Delporte

Grégoire

et al. 2013

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. Aquaporins (AQPs), a family of transmembrane water channel proteins, are essential for allowing passive water transport through retinal pigmented epithelial (RPE) cells. Even though human native RPE cells and immortalized human RPEs have been shown to express AQPs, the expression of AQPs during the differentiation in stem cell-derived RPE remains to be elucidated. METHODS.In human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs)-derived RPE cells, the expression of several AQPs was determined by quantitative real-time PCR and the localization of AQP1 was assessed with confocal microscopy. The functionality of AQP water channels was determined by cell volume assay in hESC-derived RPE cells.RESULTS. AQP1, AQP3, AQP4, AQP5, AQP6, AQP7, AQP10, AQP11, and AQP12 were expressed in hESC-and hiPSC-derived RPE cells. Furthermore, the expression of AQP1 and AQP11 genes were significantly upregulated during the maturation of both hESC and iPSC into RPE. Confocal microscopy shows the expression of AQP1 at the apical plasma membrane of polarized cobblestone hESC-and hiPSC-derived RPE cells. Lastly, aquaporin inhibitors significantly reduced AQP functionality in hESC-RPE cells.CONCLUSIONS. hESC-RPE and hiPSC-RPE cells express several AQP genes, which are functional in mature hESC-derived RPE cells. The localization of AQP1 on the apical plasma membrane in mature RPE cells derived from both hESC and hiPSC suggests its functionality. These data propose that hESC-and hiPSC-derived RPE cells, grown and differentiated under serum-free conditions, resemble their native counterpart in the human eye.

show abstract

Aquaporins 6-12 in the human eye

Cited by 36 publications

References 27 publications

Aquaporins in the eye: Expression, function, and roles in ocular disease

Aquaporins in the eye: Expression, function, and roles in ocular disease

A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues

Aquaporin Expression and Function in Human Pluripotent Stem Cell–Derived Retinal Pigmented Epithelial Cells

Contact Info

Product

Resources

About