Aquaporin water channels AQP1 and AQP3, are expressed in equine articular chondrocytes

Mobasheri, Ali; Trujillo, E.; Bell, Susan Groag; Carter, Stuart; Clegg, Peter; Martín‐Vasallo, Pablo; Marples, David

doi:10.1016/j.tvjl.2003.08.001

Cited by 60 publications

(43 citation statements)

References 20 publications

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“…Immunohistochemistry was performed according to the procedure previously described with minor modification [13]. 4-5 µm-thick serial sections were collected on poly-L-lysine-coated slides (Sigma-Aldrich, St. Louis, MO, USA) and incubated overnight at 56ºC.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Investigation of Age-Related Changes in Expression of Aquaporin-1 and Aquaporin-3 in Rat Bone

Sapmaz¹

2016

Ann Clin Anal Med

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ÖzetAmaç: Aquaporinler su ve iyon transportu sağlayan membran kanal proteinleridir. Yaşlanma ile dokulardaki aquaporinlerin miktarı değişmektedir. Aquaporin-1 (AQP1) ve aquaporin-3 (AQP3)'ün kıkırdak dokuda yaşlanma ile azaldığı gösterilmiştir. Ancak kemik dokuda yaşlanma ile aquaporinlerin miktarında değişim gösterilmemiştir. Bu çalışmada genç ve yaşlı hayvanların kemik dokularında AQP1, AQP3 ve tip I kollajen ekspresyonlarının immünohis-tokimyasal olarak araştırılması amaçlandı. Gereç ve Yöntem: Bu çalışmada 14 adet Wistar Albino cinsi sıçan kullanıldı. Grup I (n=7) iki aylık genç sıçan-lardan, Grup II (n=7) onsekiz aylık yaşlı sıçanlardan oluşmaktaydı. Kemik dokusu (femur) histopatolojik ve immunohistokimyasal değerlendirme için alın-dı. Rutin histolojik prosedürün ardından parafine gömülen dokulardan 4-5 µm kalınlığında kesitler alındı. İmmunohistokimyasal olarak AQP1, AQP3 ve tip I kollajen boyama ayrıca Hematoksilen-eozin boyama yapıldı. Bulgular: Genç ve yaşlı sıçanlarda immünohistokimyasal olarak AQP1, AQP3 ve tip I kollajen ekspresyonu gösterildi. Yaşlanma ile AQP1, AQP3 ve tip I kollajen miktarında azalma olduğu gözlendi. Tartışma: AQP1 ve AQP3 mik tarındaki azalmanın kemik dokuda yaşa bağlı değişikliklerle ilgili olabilece ği kanaatindeyiz. Anahtar KelimelerSıçan; Kemik Dokusu; Yaşlanma; Aquaporin 1; Aquaporin 3 Özet Aim: Aquaporins are membrane channel proteins that transport water and ions. The amount of aquaporins in tissue changes with age. The amount of aquaporin-1 (AQP1) and aquaporin-3 (AQP3) is considered to decrease in cartilage tissue with age. However, no age-related change has been reported regarding the amount of aquaporins in bone tissue. In this study, our aim was to examine expressions of AQP1, AQP3, and type I collagen immunohistochemically in the bone tissues of young and old rats. Material and Method: Fourteen Wistar Albino rats were included in this study. Group I (n=7) consisted of young rats that were two months old, while Group II (n=7) consisted of old rats that were eighteen months old. Bone tissue (femur) was dissected and examined histopathologically and immunohistochemically. After routine histological procedure, sections at 4-5 µm thickness were obtained from tissues embedded in paraffin. Sections were stained immunohistochemically for AQP1, AQP3, and type I collagen as well as with hematoxylin-eosin. Results: Immunohistochemically, expressions of AQP1, AQP3, and type I collagen were demonstrated in both young and old rats. AQP1, AQP3, and type I collagen amounts were found to decrease with aging. Discussion: Our findings suggest that reduced amounts of AQP1 and AQP3 may be related to agerelated changes in bone tissue.

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Section: Immunohistochemistrymentioning

confidence: 99%

Investigation of Age-Related Changes in Expression of Aquaporin-1 and Aquaporin-3 in Rat Bone

Sapmaz¹

2016

Ann Clin Anal Med

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“…Aquaporins (AQP) are water channel proteins that allow water to move in the direction of osmotic gradient and may also allow small solutes to pass, for example glycerol and urea. A role in cell volume regulation and mechanotransduction in chondrocytes has been proposed (Mobasheri et al, 2004). AQP1 and AQP3 are expressed in cartilage resulting in water permeability and may respond to environment changes since changes in aquaporin expression may be important in pathology.…”

Section: Osmotic Sensitivity Of Chondrocytesmentioning

confidence: 99%

Cellular Physiology of Articular Cartilage in Health and Disease

Milner¹,

Wilkins²,

Gibson³

2012

Principles of Osteoarthritis- Its Definition, Character, Derivation and Modality-Related Recognition

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“…A similar AQP1 is physiologically expressed in normal human temporomandibular joint (TMJ) disc 4 , and expressed and up-regulated in human TMJ disc with internal derangement 5 . In knee tissue, AQP1 is expressed in human synovitis 10 , in normal articular chondrocytes 11 and in chondrocytes of patients with rheumatoid arthritis (RA) 12 and osteoarthritis (OA) [12][13][14][15] . Over 70% of the total tissue weight in the cartilage matrix consists of water, and in earlystage OA, there is an increase in water content through swelling 16 .…”

Section: Introductionmentioning

confidence: 99%

The role of AQP1 in knee Osteoarthritis (OA): A contemporary review

Musumeci¹

2013

OA Arthritis

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IntroductionThe aim of this article is to critically review literature about aquaporin 1 and its involvement in joint diseases. In particular, we focused our research on osteoarthritis of the knee. We reviewed the literature regarding aquaporin 1 role in joint cavity disease. We examined the current literature through searching PubMed and Scopus using appropriate keywords in relation to aquaporin 1 and osteoarthritis. Main research articles were selected for review. Discussion Aquaporin 1 is an integral membrane protein expressed in different normal and pathological tissues, for example, erythrocytes, renal tubule cells and in knee tissues (synoviocytes, articular chondrocytes and fibrochondrocytes). In knee tissues, aquaporin 1 is also expressed in human synovitis and in chondrocytes of patients with rheumatoid arthritis and osteoarthritis. The process of development and ageing of articular cartilage is related to its nutrition and function, which depend mainly on the water molecular transmembrane transport. Water transport is taken by aquaporins. The regulation of the water and solute content of chondrocytes will profoundly affect their anabolic and catabolic functions. The chondrocytes are supplied by diffusion, helped by the pumping action generated by compression of the articular cartilage or flexion of the elastic cartilage. The water content of cells is effectively influenced by the abundance of aquaporin water channels. Conclusion This review reports recent findings about the involvement of aquaporin 1 in the function of the articular cartilage and its possible role in knee osteoarthritis.

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Aquaporin water channels AQP1 and AQP3, are expressed in equine articular chondrocytes

Cited by 60 publications

References 20 publications

Investigation of Age-Related Changes in Expression of Aquaporin-1 and Aquaporin-3 in Rat Bone

Investigation of Age-Related Changes in Expression of Aquaporin-1 and Aquaporin-3 in Rat Bone

Cellular Physiology of Articular Cartilage in Health and Disease

The role of AQP1 in knee Osteoarthritis (OA): A contemporary review

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