Aquaporin 7 expression in postimplantation mouse uteri: a potential role for glycerol transport in uterine decidualization

Peng, Hongying; Zhang, Ying; Li, Lei; Chen, Qi; Yue, Jingwen; Tan, Yi; Duan, Enkui

doi:10.1016/j.fertnstert.2010.11.011

Cited by 16 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of AQP7 , which showed a transient increase in abundance 8 days post‐ovulation in the present study, is expressed in mouse uteri undergoing decidualization after implantation (Peng et al. ). Neither porcine nor murine uterine tissue was found to express this aquaporin subtype (Richard et al.…”

Section: Discussionsupporting

confidence: 56%

Expression of Aquaporin Water Channels in Equine Endometrium is Differentially Regulated During the Oestrous Cycle and Early Pregnancy

Klein

Troedsson

Rutllant

2012

Reprod Domestic Animals

View full text Add to dashboard Cite

The expression of 12 different aquaporin subtypes in equine endometrium was examined at the mRNA and protein level. Endometrial samples were obtained during anoestrus, oestrus, 8, and 14 days after ovulation in non-pregnant mares, and 14 days after ovulation in pregnant mares. Quantitative PCR revealed a time-dependent pattern for all aquaporin subtypes examined except for AQP10 and 12. AQP3, 5 and 7 showed highest mRNA abundance 8 days after ovulation, while AQP0 and 2 were most abundant at Day 14 of the cycle in non-pregnant mares. At 14 days of pregnancy, AQP1, 4, 8, 9 and 11 displayed highest expression levels. Western blot analysis confirmed protein expression of AQP0, 2 and 5. Immunohistochemistry localized protein expression to luminal and glandular epithelial and stromal cells. AQP0 staining intensity was highest in samples obtained on Day 14 of the oestrous cycle. AQP2 immunoreactivity seemed to be stronger in samples collected 14 days after ovulation from non-pregnant animals, in particular luminal epithelial staining. Samples collected 8 days after ovulation from cyclic animals were characterized by intense AQP5 staining of glandular epithelium, predominantly in the deeper glands. Progesterone treatment of anoestrous mares did not enhance expression of AQPs, indicating that factors other than progesterone are required for the up-regulation of certain AQP subtypes during dioestrus. In conclusion, it seems that an equine-specific collaboration of aquaporin subtypes contributes to changes in endometrial fluid content occurring throughout the oestrous cycle and contributes to endometrial receptivity during early pregnancy in the mare.

show abstract

Section: Discussionsupporting

confidence: 56%

Expression of Aquaporin Water Channels in Equine Endometrium is Differentially Regulated During the Oestrous Cycle and Early Pregnancy

Klein

Troedsson

Rutllant

2012

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…The highest expression levels of AQP2 in the human endometrium are at the mid-secretory phases, suggesting that AQP2 may enhance endometrial receptivity to embryo implantation (Zou et al 2011). Previous studies have shown that AQP7 was dynamically expressed in mouse uteri undergoing decidualisation after implantation (Peng et al 2011), but AQP2 has not been studied in regards to participation in embryo implantation. In this study, blockage of AQP2 by siRNA and PCMB led to decreased expression of the receptive indicators LIF and ITGB3 and impaired endometrial receptivity, suggesting an important role for AQP2 in implantation.…”

Section: Discussionmentioning

confidence: 99%

Decreased expression of aquaporin 2 is associated with impaired endometrial receptivity in controlled ovarian stimulation

Zhang

et al. 2016

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Recently, there has been evidence of decreased implantation rates with in vitro fertilisation and embryo transfer due to controlled ovarian stimulation (COS). The aim of this study was to investigate the effect of COS on embryo implantation and the role of aquaporin 2 (AQP2). We recruited eight patients who underwent COS and 40 matched controls. Endometrial samples were collected on Day 4~8 after injection of human chorionic gonadotrophin in the COS group and in the mid-secretory phase in the control group. Human endometrial morphological changes after COS were examined and expression of AQP2, leukaemia inhibitory factor (LIF) and integrin B3 (ITGB3) were determined by quantitative polymerase chain reaction, western blotting and immunohistochemistry in human endometrium and Ishikawa cells. Attachment rates were obtained using the embryo attachment test. The results showed that endometrial epithelial cells from the COS group were disrupted and lacked pinopodes. Messenger RNA and protein levels of AQP2, LIF and ITGB3 decreased in endometrial samples from the COS group. Knockdown of AQP2 resulted in reduced expression of LIF and ITGB3 and reduced embryo attachment rates. In conclusion, impaired endometrial receptivity in patients who underwent COS is correlated with a decreased expression of AQP2.

show abstract

“…33 The AQP7, an aquagly-ceroporin-facilitated glycerol transport, shows a specific expression in the decidual zone during the decidualization process, suggesting that the increased requirement in glycerol utilization supports energy during decidualization. 34 However, the effect of secretin on regulation of AQP7 still needs further verification. Saline drinking and dehydration can also stimulate secretin expression.…”

Section: Discussionmentioning

confidence: 99%

Progesterone Regulates Secretin Expression in Mouse Uterus During Early Pregnancy

Huang

Wang

et al. 2014

Reprod. Sci.

View full text Add to dashboard Cite

Secretin, a classical gastrointestinal and neuroendocrine peptide, plays an important role in maintaining the body fluid balance. However, the expression and regulation of secretin in the reproductive system are still unknown. In our study, secretin is specifically expressed in the decidua on days 5 to 8 of pregnancy. Secretin expression is not detected under delayed implantation but is stimulated after estrogen activation and under artificial decidualization. Progesterone induces secretin expression in ovariectomized mice and cultured stromal cells, which is abrogated by specific LY294002. Because secretin is mainly localized in the decidua and also strongly expressed during in vitro decidualization, secretin may play a role during mouse decidualization through regulating cyclic adenosine monophosphate level.

show abstract

Aquaporin 7 expression in postimplantation mouse uteri: a potential role for glycerol transport in uterine decidualization

Cited by 16 publications

References 33 publications

Expression of Aquaporin Water Channels in Equine Endometrium is Differentially Regulated During the Oestrous Cycle and Early Pregnancy

Expression of Aquaporin Water Channels in Equine Endometrium is Differentially Regulated During the Oestrous Cycle and Early Pregnancy

Decreased expression of aquaporin 2 is associated with impaired endometrial receptivity in controlled ovarian stimulation

Progesterone Regulates Secretin Expression in Mouse Uterus During Early Pregnancy

Contact Info

Product

Resources

About