Apyrase and 5′-nucleotidase Activities in Synaptosomes from the Cerebral Cortex of Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon-β

Spanevello, Rosélia Maria; Mazzanti, Cinthia M.; Kaizer, Rosilene Rodrigues; Zanin, Rafael Fernandes; Cargnelutti, Denise; Hannel, Laura; Junior, Marcos Corrêa; Mazzantí, Alexandre; Festugatto, Rafael; Graça, Dominguita Lühers; Schetinger, Maria Rosa Chitolina; Morsch, Vera Maria

doi:10.1007/s11064-006-9039-5

Cited by 24 publications

(14 citation statements)

References 41 publications

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“…2 and 3). These results corroborate with previous studies from our laboratory showing that other enzymes, such as apyrase, also have their activities altered in synaptosomes after demyelination by EB [42].…”

Section: Discussionsupporting

confidence: 93%

Acetylcholinesterase Activity in Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon Beta

et al. 2006

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The ethidium bromide (EB) demyelinating model was associated with interferon beta (IFN-beta) to evaluate acetylcholinesterase (AChE) activity in the striatum (ST), hippocampus (HP), cerebral cortex (CC), cerebellum (CB), hypothalamus (HY), pons (PN) and synaptosomes from the CC. Rats were divided into four groups: I control (saline), II (IFN-beta), III (EB) and IV (EB and IFN-beta). After 7, 15 and 30 days rats (n = 6) were sacrificed, and the brain structures were removed for enzymatic assay. AChE activity was found to vary in all the brain structures in accordance with the day studied (7-15-30 days) (P < 0.05). In the group III, there was an inhibition of the AChE activity in the ST, CB, HY, HP and also in synaptosomes of the CC (P < 0.05). It was observed that IFN-beta per se was capable to significantly inhibit (P < 0.05) AChE activity in the ST, HP, HY and synaptosomes of the CC. Our results suggest that one of the mechanisms of action of IFN-beta is through the inhibition of AChE activity, and EB could be considered an inhibitor of AChE activity by interfering with cholinergic neurotransmission in the different brain regions.

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Section: Discussionsupporting

confidence: 93%

Acetylcholinesterase Activity in Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon Beta

et al. 2006

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“…The results of this study revealed that ADA activity was considerably increased in the kidney of arsenic only treated rats compared with the control (p < 0.05). Earlier researches have confirmed upregulation in the ADA activity in renal injury (29,30,31). Hence, increase ADA activity found in this study might lead to a reduction in the level of adenosine, a renoprotector molecule (15,16).…”

Section: Discussionsupporting

confidence: 76%

Aqueous Extract of Carica Papaya Linn Roots Halts Sodium Arsenite-Induced Renal Inflammation through Inhibiting Adenosine Deaminase, 8-Hydroxy-2′-Deoxyguanosine, C-Reactive Protein and Inducible Nitric Oxide Synthase Activity

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Osukoya

et al. 2017

Serbian Journal of Experimental and Clinical Research

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Objectives: Infl ammation plays a crucial role in many of the metabolic abnormalities. Th e prototypic marker of infl ammation is C-reactive protein (CRP), Nitric Oxide (NO), inducible nitric oxide synthase (iNOS) and their inhibition is considered a promising SAŽETAK Ciljevi: Infl amacija ima ključnu ulogu u brojnim metaboličkim poremećajima. Smatra se da inhibicija faktora infl amacije kao što su C-reaktivni protein (CRP), azot monoksid (NO), inducibilna azot monoksid sintetaza (iNOS) predstavlja obećavajuću strategiju u borbi sa infl amacijom. U ovom radu smo izvestili o antiinfl amatornom mehanizmu vodenog ekstrakta korena Carica papaya kod natrijum-arsenitom izazvane bubrežne disfunkcije.Metodologija: U eksperimentima je korišćeno 35 pacova. Griess-ov rastvor je korišćen da ispita inhibitorni efekat vodenog ekstrakta korena Carica papaya na povećanu produkciju azot monoksida. Za određivanje nivoa proinfl amatornog markera CRP-a je korišćena elisa. Elisa je korišćena za analizu

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“…We observed that EB selectively destroyed glial cells (oligodendrocytes and astrocytes), which control the processes of demyelination and remyelination [55,87,88]. The results demonstrated that NTPDase and 5'nucleotidase activities were increased in synaptosomes [120] and reduced in platelets [87,119,120], indicating that there is an interaction between these enzymes and demyelinating events.…”

Section: Ectonucleotidases and Demyelinationmentioning

confidence: 85%

NTPDase and 5'‐nucleotidase activities in physiological and disease conditions: New perspectives for human health

et al. 2007

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Abstract. Extracellular nucleotides and nucleosides act as signaling molecules involved in a wide spectrum of biological effects. Their levels are controlled by a complex cell surface-located group of enzymes called ectonucleotidases. There are four major families of ectonucleotidases, nucleoside triphosphate diphosphohydrolases (NTPDases/CD39), ectonucleotide pyrophosphatase/phosphodiesterases (E-NPPs), alkaline phosphatases and ecto-5'-nucleotidase. In the last few years, substantial progress has been made toward the molecular identification of members of the ectonucleotidase families and their enzyme structures and functions. In this review, there is an emphasis on the involvement of NTPDase and 5'-nucleotidase activities in disease processes in several tissues and cell types. Brief background information is given about the general characteristics of these enzymes, followed by a discussion of their roles in thromboregulatory events in diabetes, hypertension, hypercholesterolemia and cancer, as well as in pathological conditions where platelets are less responsive, such as in chronic renal failure. In addition, immunomodulation and cell-cell interactions involving these enzymes are considered, as well as ATP and ADP hydrolysis under different clinical conditions related with alterations in the immune system, such as acute lymphoblastic leukemia (ALL), Bchronic lymphocytic leukemia (B-CLL) and infections associated with human immunodeficiency virus (HIV). Finally, changes in ATP, ADP and AMP hydrolysis induced by inborn errors of metabolism, seizures and epilepsy are discussed in order to highlight the importance of these enzymes in the control of neuronal activity in pathological conditions. Despite advances made toward understanding the molecular structure of ectonucleotidases, much more investigation will be necessary to entirely grasp their role in physiological and pathological conditions.

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Apyrase and 5′-nucleotidase Activities in Synaptosomes from the Cerebral Cortex of Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon-β

Cited by 24 publications

References 41 publications

Acetylcholinesterase Activity in Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon Beta

Acetylcholinesterase Activity in Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon Beta

Aqueous Extract of Carica Papaya Linn Roots Halts Sodium Arsenite-Induced Renal Inflammation through Inhibiting Adenosine Deaminase, 8-Hydroxy-2′-Deoxyguanosine, C-Reactive Protein and Inducible Nitric Oxide Synthase Activity

NTPDase and 5'‐nucleotidase activities in physiological and disease conditions: New perspectives for human health

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