Aptasensors for viral diagnostics

Kieboom, Corné van den; Beek, Samantha L. van der; Mészáros, Tamás; Gyurcsányi, Róbert E.; Ferwerda, Gerben; Jonge, Marien I. de

doi:10.1016/j.trac.2015.05.012

Cited by 47 publications

(27 citation statements)

References 95 publications

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“…Aptamers are also much more stable than antibodies making them suitable in applications requiring harsh conditions, (e.g., high temperature or extreme pH) [143]. General review articles about aptamer-based sensors [144] and aptamer-based sensors for viruses are available in literature [145][146][147].…”

Section: Aptamersmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Section: Aptamersmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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“…Both label-based and label-free affinity biosensors are used for viral detection, including immunosensors, DNA-sensors and aptamer-based sensors (aptasensors) ( Fig. 1 ) ( Mollarasouli et al, 2019 ; Van der Kieboom et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Developments in biosensors for CoV detection and future trends

Antiochia

2021

Biosensors and Bioelectronics

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This review summarizes the state of art of biosensor technology for Coronavirus (CoV) detection, the current challenges and the future perspectives. Three categories of affinity-based biosensors (ABBs) have been developed, depending on their transduction mechanism, namely electrochemical, optical and piezoelectric biosensors. The biorecognition elements include antibodies and DNA, which undergo important non-covalent binding interactions, with the formation of antigen-antibody and ssDNA/oligonucleotide-complementary strand complexes in immuno- and DNA-sensors, respectively. The analytical performances, the advantages and drawbacks of each type of biosensor are highlighted, discussed, and compared to traditional methods. It is hoped that this review will encourage scientists and academics to design and develop new biosensing platforms for point-of-care (POC) diagnostics to manage the coronavirus disease 2019 (COVID-19) pandemic, providing interesting reference for future studies.

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“…detection of virus-specific antibodies generated in response to the infection). 1 Direct detection certainly prevails to characterize and standardize virus preparations, and is also used for medical diagnostics because it enables genotyping and is more specific than the measurement of host immune responses (e.g. it is not restricted by seroconversion times, co-morbidities or treatments).…”

Section: Introductionmentioning

confidence: 99%

“…it is not restricted by seroconversion times, co-morbidities or treatments). 1 However, the majority of direct detection methodologies target only a single component of the virus, i.e., characteristic nucleic acids and proteins even though virus pathogenesis involves whole virus particles. 2 Therefore, the interpretation of the results may be complicated if the detected viral elements are not linked to intact viral particles but originate from disintegrated viruses or contaminations.…”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, the virus particles can be identified as fluorescent particles, sized and quantified (Scheme 1). Currently, there is strong interest in synthetic receptors for viruses as alternatives to antibodies 1,18 due to their better stability and versatility in terms of custom chemical modification. Therefore, for the fluorescence detection of virus particles, we took advantage of monovalent fluorescently labeled aptamers to rule out virus aggregation.…”

Section: Introductionmentioning

confidence: 99%

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Selective counting and sizing of single virus particles using fluorescent aptamer-based nanoparticle tracking analysis

et al. 2018

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Detection and counting of single virus particles in liquid samples are largely limited to narrow size distribution of viruses and purified formulations. To address these limitations, here we propose a calibration-free method that enables concurrently the selective recognition, counting and sizing of virus particles as demonstrated through the detection of human respiratory syncytial virus (RSV), an enveloped virus with a broad size distribution, in throat swab samples. RSV viruses were selectively labeled through their attachment glycoproteins (G) with fluorescent aptamers, which further enabled their identification, sizing and counting at the single particle level by fluorescent nanoparticle tracking analysis. The proposed approach seems to be generally applicable to virus detection and quantification. Moreover, it could be successfully applied to detect single RSV particles in swab samples of diagnostic relevance. Since the selective recognition is associated with the sizing of each detected particle, this method enables to discriminate viral elements linked to the virus as well as various virus forms and associations.

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Aptasensors for viral diagnostics

Cited by 47 publications

References 95 publications

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Developments in biosensors for CoV detection and future trends

Selective counting and sizing of single virus particles using fluorescent aptamer-based nanoparticle tracking analysis

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