Aptasensors for Small Molecule Detection

Walter, Johanna‐Gabriela; Heilkenbrinker, Alexandra; Austerjost, Jonas; Tımur, Suna; Stahl, Frank; Schepe, Thomas

doi:10.5560/znb.2012-0147

Cited by 37 publications

(38 citation statements)

References 31 publications

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“…In comparison to antibodies, aptamers have a high stability, a straight forward modification potential and lack of immunogenicity [24,25]. Aptamers are recovered by a procedure named SELEX (Systematic Evolution of Ligands by Exponential Enrichment) [26]. This procedure selects those oligonucleotides which are binding the desired target with the highest affinity [26,27].…”

Section: Introductionmentioning

confidence: 99%

“…Aptamers are recovered by a procedure named SELEX (Systematic Evolution of Ligands by Exponential Enrichment) [26]. This procedure selects those oligonucleotides which are binding the desired target with the highest affinity [26,27]. The mentioned characteristics encouraged us to use the aptamers S15 and S6 which specifically bind to lung cancer cells as a ligand for the model targeted drug delivery system [25].…”

Section: Introductionmentioning

confidence: 99%

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Aptamer-modified polymer nanoparticles for targeted drug delivery

et al. 2016

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Abstract:The purpose of this study was to develop a model system for targeted drug delivery. This system should enable targeted drug release at a certain tissue in the body. In conventional drug delivery systems, drugs are often delivered unspecifically resulting in unwarranted adverse effects. To circumvent this problem, there is an increasing demand for the development of intelligent drug delivery systems allowing a tissue-specific mode of delivery. Within this study, nanoparticles consisting of two biocompatible polymers are used. Because of their small size, nanoparticles are well-suited for effective drug delivery. The small size affects their movement through cell and tissue barriers. Their cellular uptake is easier when compared to larger drug delivery systems. Paclitaxel was encapsulated into the nanoparticles as a model drug, and to achieve specific targeting an aptamer directed against lung cancer cells was coupled to the nanoparticles surface. Nanoparticles were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and nanotracking analysis (NTA). Also their surface charge was characterized from ζ-potential measurements. Their preparation was optimized and subsequently specificity of drugloaded and aptamer-functionalized nanoparticles was investigated using lung cancer cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aptamer-modified polymer nanoparticles for targeted drug delivery

et al. 2016

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“…Thus, aptamers become important components in the design of biosensors, and aptamer-based sensors have been widely usded for targeting small molecule [22], such as cocaine [23], organophosphorus pesticides [24,25], heavy metals [26], pharmaceuticals [27,28], due to the considerable advantages, such as thermal stability, simpler preparation, lower-priced production cost, high selectivity and sensitivity for targets [22,29]. Until now, EA aptamer, one of the smallest aptamer targets so far, has been in vitro-selected [30], but few aptamer sensors have been constructed for EA detection.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Aptamer-based biosensor for label-free detection of ethanolamine by electrochemical impedance spectroscopy

Liang

Man

Jin

et al. 2016

Analytica Chimica Acta

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“…Major future trends for biosensor technology comprise the development and use of new biocomponents, like thermostable enzymes or aptamer structures (as thermostable alternatives to antibodies). Aptamers are short, single-stranded DNA or RNA oligonucleotides forming a unique three-dimensional structure that is highly specific to the ligand [45][46][47][48][49].…”

Section: Biosensorsmentioning

confidence: 99%

Sensor systems for bioprocess monitoring

Biechele

Busse

Solle

et al. 2015

Engineering in Life Sciences

168

158

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The ability to measure all process variables is of great importance in the field of bioprocess monitoring and control, and continuous, real‐time measurements are highly desired. The more complete and real‐time the measurements are, the more stable, reproducible, and efficient the process can be, leading to reproducibly high‐quality products. This additional information allows the operator to better document the entire process. The process analytical technologies initiative of the US Food and Drug Administration is strongly related to the analysis and control of biopharmaceutical processes. The aim of the initiative is to create processes, generating products of ensured quality by measuring quality‐related process variables. The quality of the product is enhanced by a deep understanding of the process, which is enabled by an effective and suitable sensor system. The aim of this review is to provide an overview of current and emerging sensors for bioprocess monitoring. Sensors directly interfaced to bioreactors for measuring important variables from the gas phase, such as oxygen and carbon dioxide concentration, are discussed, as well as sensors for the monitoring of the biomass concentration and morphology and of the changing medium composition. Furthermore, sensor systems are discussed. These involve sensors (especially biosensors) that are not implemented directly inside the bioreactor but rather are used in conjunction with sample‐taking systems such as flow injection analysis. A major focus is given to spectroscopic sensors, which are noninvasive and offer interesting options for a simultaneous analysis of various compounds. Since data handling is extremely important for this kind of sensor, chemometrics are also included. Soft sensors are discussed as technology that allows a user to incorporate more process data as it become available. Finally, the current state of disposable sensor technology is presented. These sensors are needed for the growing area of disposable and continuous biomanufacturing.

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Aptasensors for Small Molecule Detection

Cited by 37 publications

References 31 publications

Aptamer-modified polymer nanoparticles for targeted drug delivery

Aptamer-modified polymer nanoparticles for targeted drug delivery

Aptamer-based biosensor for label-free detection of ethanolamine by electrochemical impedance spectroscopy

Sensor systems for bioprocess monitoring

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