Aptasensors for pesticide detection

Liu, Mei; Khan, Arshad; Wang, Zhifei; Liu, Yuan; Yang, Gaojian; Deng, Yan; He, Nongyue

doi:10.1016/j.bios.2019.01.006

Cited by 214 publications

(79 citation statements)

References 156 publications

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“…Aptamers are short, single-stranded oligonucleotide sequences (DNA, RNA, or nucleic acid analogs) selected from a nucleic acid molecular library using the in vitro systematic evolution of ligands by exponential enrichment (SELEX) method (Stoltenburg et al, 2007;Abnous et al, 2017b;Alizadeh et al, 2018). Owing to their dimensional folded configurations, aptamers possess a high specificity and affinity for specific targets, including mycotoxins, pathogens, metal ions, pesticides, and cells (Meng et al, 2015;Danesh et al, 2018;Liu et al, 2019;Wu et al, 2019c,d;Yu S.H. et al, 2019).…”

Section: Aptasensors For Aflatoxin B1 From Biofilmmentioning

confidence: 99%

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

Wang

Yang

2020

Front. Microbiol.

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Aspergillus exists commonly in many crops and any process of crop growth, harvest, storage, and processing can be polluted by this fungus. Once it forms a biofilm, Aspergillus can produce many toxins, such as aflatoxin B1 (AFB1), ochratoxin, zearalenone, fumonisin, and patulin. Among these toxins, AFB1 possesses the highest toxicity and is labeled as a group I carcinogen in humans and animals. Consequently, the proper control of AFB1 produced from biofilms in food and feed has long been recognized. Moreover, many biosensors have been applied to monitor AFB1 in biofilms in food. Additionally, in recent years, novel molecular recognition elements and transducer elements have been introduced for the detection of AFB1. This review presents an outline of recent progress made in the development of biosensors capable of determining AFB1 in biofilms, such as aptasensors, immunosensors, and molecularly imprinted polymer (MIP) biosensors. In addition, the current feasibility, shortcomings, and future challenges of AFB1 determination and analysis are addressed.

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Section: Aptasensors For Aflatoxin B1 From Biofilmmentioning

confidence: 99%

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

Wang

Yang

2020

Front. Microbiol.

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“…Therefore, benefits from the booming development of various aptasensors for fast, selective, sensitive and on-site targets detection have attracted much attention. Aptamers as the molecular recognitions are at the center of aptasensors, however, up to date, there are only several target-specific aptamers developed for development of aptasensors and some of those aptamers lack selectivity, which is not able to distinguish among analogues [26]. Thus, more highly target analytes-specific aptamers need to be selected.…”

Section: Introductionmentioning

confidence: 99%

SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding

et al. 2020

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Background: Aptamers, single-stranded DNAs or RNAs, can be selected from a library containing random sequences using a method called Systematic Evolution of Ligands by EXponential Enrichment (SELEX). In SELEX, monitoring the enriching statuses of aptamer candidates during the process is a key step until today. Conformational change of an aptamer caused by target-binding in gel can be used to indicate its statuses of binding. Results: In this study, an easy-to-implement gel-based diffusion method (GBDM) was developed to monitor the interaction between enriched aptamer candidates and their targets. In order to prove the concept, characterization of aptamers targeting their targets including protein (thrombin) and non-protein molecules (acetamiprid, ATP, atrazine, profenofos and roxithromycin), respectively, were performed using mini gels. Our method has advantages over the common methods including easy performed with labor-and time-saving in experimental operation. The concept has been proven by monitoring enrichment of dynamic aptamer candidate libraries targeting a small molecule 2,2-bis(4-chlorophenyl) acetic acid (DDA) during SELEX process. A mini gel cassette was designed and fabricated by our laboratory to make mini agarose gels for diffusion with different directions. Conclusions:These results indicate that GBDM, in particular, chasing diffusion is suitable for monitoring the interaction between enriched aptamer candidates and their targets. These pioneering efforts are helpful for novel aptamer selection by breaking through the technical bottleneck of aptamer development and helpful for development of novel aptasensors.

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“…cancer) to infectious (e.g. HIV, malaria and sepsis), as well as the presence of poisons like pesticides and is an important means for determining the presence of antibiotic resistance (Liu et al, 2019;Kumar, 2019;Diculescu, 2005; Bartosik and Jirakova, 2019). Combined with the benefits of microfabrication and lab-on-a-chip approaches, it is not surprising that electrochemical DNA sensors for biomedical applications are a popular area of research.…”

Section: Introductionmentioning

confidence: 99%

A review of microfabricated electrochemical biosensors for DNA detection

Blair

Corrigan

2019

Biosensors and Bioelectronics

119

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This review article presents an overview of recent work on electrochemical biosensors developed using microfabrication processes, particularly sensors used to achieve sensitive and specific detection of DNA sequences. Such devices are important as they lend themselves to miniaturisation, reproducible mass-manufacture, and integration with other previously existing technologies and production methods. The review describes the current state of these biosensors, novel methods used to produce them or enhance their sensing properties, and pathways to deployment of a complete point-ofcare biosensing system in a clinical setting.

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Aptasensors for pesticide detection

Cited by 214 publications

References 156 publications

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding

A review of microfabricated electrochemical biosensors for DNA detection

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