Aptasensors for lysozyme detection: Recent advances

Melinte, Gheorghe; Selvolini, Giulia; Cristea, Cécilia; Marrazza, Giovanna

doi:10.1016/j.talanta.2021.122169

Cited by 39 publications

(19 citation statements)

References 95 publications

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“…The anti-lysozyme aptamers exploited in literature and adopted in this study are derived from two independent SELEX selections [ 20 , 25 , 26 ]: A80 and C80 are two full-length sequences, containing two fixed primer sequences (flanking regions), one on each side of a central domain that is randomized; A40 and C30 are derived from A80 and C80, respectively, by removing the primers. A80R and C80R are DNA oligonucleotides used as negative control: in particular, they are derived from randomization of the central region of A80 and C80, respectively, in order to simulate the randomization process involved in the library construction during SELEX process.…”

Section: Resultsmentioning

confidence: 99%

“…Considering egg white lysozyme (14 kDa), different DNA aptamers have been exploited for the development of aptasensors for its determination in different food and biological matrices [ 20 ]. However, for the development of aptamer-based analytical methods, the choice of the aptamer sequence and binding conditions is commonly made regardless of the binding buffer used for SELEX selection or the downstream sample matrix.…”

Section: Introductionmentioning

confidence: 99%

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FFF-based high-throughput sequence shortlisting to support the development of aptamer-based analytical strategies

et al. 2022

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Aptamers are biomimetic receptors that are increasingly exploited for the development of optical and electrochemical aptasensors. They are selected in vitro by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure, but although they are promising recognition elements, for their reliable applicability for analytical purposes, one cannot ignore sample components that cause matrix effects. This particularly applies when different SELEX-selected aptamers and related truncated sequences are available for a certain target, and the choice of the aptamer should be driven by the specific downstream application. In this context, the present work aimed at investigating the potentialities of asymmetrical flow field-flow fractionation (AF4) with UV detection for the development of a screening method of a large number of anti-lysozyme aptamers towards lysozyme, including randomized sequences and an interfering agent (serum albumin). The possibility to work in native conditions and selectively monitor the evolution of untagged aptamer signal as a result of aptamer-protein binding makes the devised method effective as a strategy for shortlisting the most promising aptamers both in terms of affinity and in terms of selectivity, to support subsequent development of aptamer-based analytical devices. Graphical abstract

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

FFF-based high-throughput sequence shortlisting to support the development of aptamer-based analytical strategies

et al. 2022

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“…Concerning the electrochemical biosensors for milk allergens and lysozyme detection, we want to suggest two recent and interesting reviews [ 64 , 65 ], presenting the development of immunosensors and aptasensors and analyzing weaknesses and strengths and future challenges.…”

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

“…Therefore, it is considered as one of the five major allergenic proteins together with OM, OVA, ovotransferrin, and ovomucin in chicken eggs. A review, published in 2021, presents the electrochemical and optical aptasensors for Lys detection in human fluids and in foods, reporting analytical performances, adaptability, and real sample applications [ 65 ], while in this review we present the most recent examples of aptasensors for Lys detection in food products.…”

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Curulli

2022

Biosensors

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Food allergy has been indicated as the most frequent adverse reaction to food ingredients over the past few years. Since the only way to avoid the occurrence of allergic phenomena is to eliminate allergenic foods, it is essential to have complete and accurate information on the components of foodstuff. In this framework, it is mandatory and crucial to provide fast, cost-effective, affordable, and reliable analysis methods for the screening of specific allergen content in food products. This review reports the research advancements concerning food allergen detection, involving electrochemical biosensors. It focuses on the sensing strategies evidencing different types of recognition elements such as antibodies, nucleic acids, and cells, among others, the nanomaterial role, the several electrochemical techniques involved and last, but not least, the ad hoc electrodic surface modification approaches. Moreover, a selection of the most recent electrochemical sensors for allergen detection are reported and critically analyzed in terms of the sensors’ analytical performances. Finally, advantages, limitations, and potentialities for practical applications of electrochemical biosensors for allergens are discussed.

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“…Emerging techniques for lysozyme measurement in biological samples include antibody [31][32][33], aptamer [34], MIP [35] or nanoparticle-based [36] biosensors and the use of core-shell polymer NPs as selective extraction sorbents in the sample cleanup process. Most of the newly developed lysozyme nanoadsorbents use a molecular imprinting strategy [37][38][39][40][41][42][43][44][45][46] and only one of them relies on a polymeric material [18] with optimized composition of special functional monomers to achieve selectivity.…”

Section: Introductionmentioning

confidence: 99%

Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples

2021

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Highly selective multifunctional magnetic nanoparticles containing a thermoresponsive polymer shell were developed and used in the sample pretreatment of urine for the assessment of lysozymuria in leukemia patients. Crosslinked poly(N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) was grown onto silica-coated magnetic nanoparticles by reversible addition fragmentation chain transfer (RAFT) polymerization. The lysozyme binding property of the nanoparticles was investigated as a function of time, protein concentration, pH, ionic strength and temperature and their selectivity was assessed against other proteins. High-abundant proteins, like human serum albumin and γ-globulins did not interfere with the binding of lysozyme even at elevated concentrations characteristic of proteinuria. A sample cleanup procedure for urine samples has been developed utilizing the thermocontrollable protein binding ability of the nanoparticles. Method validation was carried out according to current bioanalytical method validation guidelines. The method was highly selective, and the calibration was linear in the 25 to 1000 µg/mL concentration range, relevant in the diagnosis of monocytic and myelomonocytic leukemia. Intra- and inter-day precision values ranged from 2.24 to 8.20% and 1.08 to 5.04%, respectively. Intra-day accuracies were between 89.9 and 117.6%, while inter-day accuracies were in the 88.8 to 111.0% range. The average recovery was 94.1 ± 8.1%. Analysis of unknown urine samples in comparison with a well-established reference method revealed very good correlation between the results, indicating that the new nanoparticle-based method has high potential in the diagnosis of lysozymuria.

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Aptasensors for lysozyme detection: Recent advances

Cited by 39 publications

References 95 publications

FFF-based high-throughput sequence shortlisting to support the development of aptamer-based analytical strategies

FFF-based high-throughput sequence shortlisting to support the development of aptamer-based analytical strategies

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples

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