Aptasensors for environmental monitoring of contaminants in water and soil

Kim, Sang Hoon; Thỏa, Trần Thị Thanh; Gu, Man Bock

doi:10.1016/j.coesh.2019.09.003

Cited by 18 publications

(9 citation statements)

References 115 publications

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“…, enzymes, antibodies, and lectin). 19–21 These advantages allow the easy generation of target-specific aptamers in vitro , under the same conditions as those used for detection, which is a clear advantage compared to other sensor elements. 22,23 Therefore, sensors aptamer-based could be used as “lab-on-a-chip” tools without specialized personnel and establishing a full laboratory system.…”

Section: Introductionmentioning

confidence: 99%

Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX

Seo

Kim

et al. 2021

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX

Seo

Kim

et al. 2021

RSC Adv.

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“…Among different biorecognition elements, aptamers are characterized by different advantages such as low cost, facile synthesis, thermostability, and shelf life [13]. Hence, the aptasensors have become attractive in the bioanalytical field for different reasons: simplicity, high sensitivity, high selectivity, low cost, and fast response [14][15][16], compared to more traditional biorecognition elements like enzymes and antibodies [17]. Aptamers are in vitro-selected single-stranded DNA or RNA that are isolated via an in vitro selection process called 'systematic evolution of ligands by exponential enrichment' (SELEX) [18].…”

Section: Introductionmentioning

confidence: 99%

“…CNTs have attained great interest in electrochemical biosensors due to their high surface area to volume ratio and stability [21], as well as due to their good conductivity. Usually, CNTs are used to increase the electron transfer between the electrolyte and the surface of the electrode [15,16,22,23]. Besides CNTs, metal nanoparticles (MNPs) [24,25] are also broadly used in electrochemical sensors due to their small dimensional scale, good stability, and excellent conductivity [25].…”

Section: Introductionmentioning

confidence: 99%

Flexible Screen Printed Aptasensor for Rapid Detection of Furaneol: A Comparison of CNTs and AgNPs Effect on Aptasensor Performance

et al. 2020

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Furaneol is a widely used flavoring agent, which can be naturally found in different products, such as strawberries or thermally processed foods. This is why it is extremely important to detect furaneol in the food industry using ultra-sensitive, stable, and selective sensors. In this context, electrochemical biosensors are particularly attractive as they provide a cheap and reliable alternative measurement device. Carbon nanotubes (CNTs) and silver nanoparticles (AgNPs) have been extensively investigated as suitable materials to effectively increase the sensitivity of the biosensors. However, a comparison of the performance of biosensors employing CNTs and AgNPs is still missing. Herein, the effect of CNTs and AgNPs on the biosensor performance has been thoughtfully analyzed. Therefore, disposable flexible and screen printed electrochemical aptasensor modified with CNTs (CNT-ME), or AgNPs (AgNP-ME) have been developed. Under optimized conditions, CNT-MEs showed better performance compared to AgNP-ME, yielding a linear range of detection over a dynamic concentration range of 1 fM–35 μM and 2 pM–200 nM, respectively, as well as high selectivity towards furaneol. Finally, our aptasensor was tested in a real sample (strawberry) and validated with high-performance liquid chromatography (HPLC), showing that it could find an application in the food industry.

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“…When considering the need for specified custom-tailored aptasensors in water analysis, POC with cost-effective, accurate, user-friendly and delicate features is a requirement for pesticide analysis. Although conventional chromatographic methods require a clean-up step for sample preparation [47] aptasensors could be used for direct detection of environmental contaminants in the spiked real water samples [48]. As one of the most commonly used pesticides, Imi has not been yet attempted for detection by using pesticide aptasensors [49].…”

Section: Introductionmentioning

confidence: 99%

in vitro Selection of Aptamer for Imidacloprid Recognition as Model Analyte and Construction of a Water Analysis Platform

et al. 2020

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Pesticide use in agriculture is one of the threats to water safety. Therefore, detection of pesticide residues is crucial for human health. Compared to conventional chromatographic methods, aptasensors are promising tools for fast, cheap and sensitive detection of environmental contaminants. To the best of our knowledge, such an aptasensor has not been reported for imidacloprid (Imi) which is one of the most widely used pesticides. In order to meet this demand, we initially selected two novel aptamers designated as ‘Apta‐1’ and ‘Apta‐2’ by graphene oxide‐SELEX (GO‐SELEX) method. Then, these aptamers were used to fabricate the gold electrode‐based aptasensor platforms and characterized by using electrochemical methods such as cyclic voltammetry, and electrochemical impedance spectroscopy as well as X‐Ray photoelectron spectroscopy. It was found that the limit of detection value of Apta‐1 based sensor for the Imi was found better than Apta‐2 based system, although linear ranges were similar. Based on that finding, Apta‐1 based system was further tested against possible interference molecules. The proposed platform was successfully used for detection of very low concentrations of Imi in the range of ng/mL. Thus, it eliminates the need for sample pre‐treatment and enables a practical analysis in real wastewater samples.

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Aptasensors for environmental monitoring of contaminants in water and soil

Cited by 18 publications

References 115 publications

Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX

Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX

Flexible Screen Printed Aptasensor for Rapid Detection of Furaneol: A Comparison of CNTs and AgNPs Effect on Aptasensor Performance

in vitro Selection of Aptamer for Imidacloprid Recognition as Model Analyte and Construction of a Water Analysis Platform

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