Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review

Yousefi, Meysam; Dehghani, Sadegh; Nosrati, Rahim; Zare, Hamed; Evazalipour, Mehdi; Mosafer, Jafar; Tehrani, Bahram Soltani; Pasdar, Alireza; Mokhtarzadeh, Ahad; Ramezani, Mohammad

doi:10.1016/j.bios.2019.01.015

Cited by 109 publications

(43 citation statements)

References 132 publications

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“…Another interesting feature of aptamers is that they effectively preserve their properties in various experimental conditions [ 149 , 150 ]. Aptasensing bio-assays have various recognition mechanisms and can bind to a wide range of regulatory proteins [ 138 , 151 ], enzymes [ 152 , 153 ], mono- and polyclonal antibodies [ 154 ], amino acids [ 155 ], growth factors [ 156 ], toxins [ 157 ], low molecular weight vitamins [ 158 ], cancer biomarkers [ 159 – 161 ], and even some metal ions [ 162 ]. Large quantities of aptamers can be biochemically synthesized most frequently through the systematic evolution of ligands by exponential enrichment (SELEX).…”

Section: Detection Methodsmentioning

confidence: 99%

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

et al. 2020

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A novel coronavirus of zoonotic origin (SARS-CoV-2) has recently been recognized in patients with acute respiratory disease. COVID-19 causative agent is structurally and genetically similar to SARS and bat SARS-like coronaviruses. The drastic increase in the number of coronavirus and its genome sequence have given us an unprecedented opportunity to perform bioinformatics and genomics analysis on this class of viruses. Clinical tests like PCR and ELISA for rapid detection of this virus are urgently needed for early identification of infected patients. However, these techniques are expensive and not readily available for point-of-care (POC) applications. Currently, lack of any rapid, available, and reliable POC detection method gives rise to the progression of COVID-19 as a horrible global problem. To solve the negative features of clinical investigation, we provide a brief introduction of the general features of coronaviruses and describe various amplification assays, sensing, biosensing, immunosensing, and aptasensing for the determination of various groups of coronaviruses applied as a template for the detection of SARS-CoV-2. All sensing and biosensing techniques developed for the determination of various classes of coronaviruses are useful to recognize the newly immerged coronavirus, i.e., SARS-CoV-2. Also, the introduction of sensing and biosensing methods sheds light on the way of designing a proper screening system to detect the virus at the early stage of infection to tranquilize the speed and vastity of spreading. Among other approaches investigated among molecular approaches and PCR or recognition of viral diseases, LAMP-based methods and LFAs are of great importance for their numerous benefits, which can be helpful to design a universal platform for detection of future emerging pathogenic viruses.

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Section: Detection Methodsmentioning

confidence: 99%

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

et al. 2020

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“…The molecular recognition layer is especially important for achieving specific detection in biomedical analyses. To achieve specific and strong binding of the analyte to the biosensor, various bioreceptors such as antibodies, aptamers, enzymes, and peptides have been used [14][15][16][17][18][19]. The physiological fluids mentioned above are complex mixtures of thousands or millions of ions, proteins, nucleic acids, and cells; therefore, the specific binding of a target analyte in physiological fluid is one of the most important challenges for increasing the sensitivity and reducing the noise of biosensors [20].…”

Section: Introductionmentioning

confidence: 99%

Surface Display Technology for Biosensor Applications: A Review

Park

2020

Sensors

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Surface display is a recombinant technology that expresses target proteins on cell membranes and can be applied to almost all types of biological entities from viruses to mammalian cells. This technique has been used for various biotechnical and biomedical applications such as drug screening, biocatalysts, library screening, quantitative assays, and biosensors. In this review, the use of surface display technology in biosensor applications is discussed. In detail, phage display, bacterial surface display of Gram-negative and Gram-positive bacteria, and eukaryotic yeast cell surface display systems are presented. The review describes the advantages of surface display systems for biosensor applications and summarizes the applications of surface displays to biosensors.

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“…A biosensor is an analytical device that combines a biological component (the so-called ‘biorecognition element’) with a physicochemical detector called a ‘transducer’ to detect specific chemical substances [ 12 ]. Among different biorecognition elements, aptamers are characterized by different advantages such as low cost, facile synthesis, thermostability, and shelf life [ 13 ]. Hence, the aptasensors have become attractive in the bioanalytical field for different reasons: simplicity, high sensitivity, high selectivity, low cost, and fast response [ 14 , 15 , 16 ], compared to more traditional biorecognition elements like enzymes and antibodies [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Flexible Screen Printed Aptasensor for Rapid Detection of Furaneol: A Comparison of CNTs and AgNPs Effect on Aptasensor Performance

et al. 2020

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Furaneol is a widely used flavoring agent, which can be naturally found in different products, such as strawberries or thermally processed foods. This is why it is extremely important to detect furaneol in the food industry using ultra-sensitive, stable, and selective sensors. In this context, electrochemical biosensors are particularly attractive as they provide a cheap and reliable alternative measurement device. Carbon nanotubes (CNTs) and silver nanoparticles (AgNPs) have been extensively investigated as suitable materials to effectively increase the sensitivity of the biosensors. However, a comparison of the performance of biosensors employing CNTs and AgNPs is still missing. Herein, the effect of CNTs and AgNPs on the biosensor performance has been thoughtfully analyzed. Therefore, disposable flexible and screen printed electrochemical aptasensor modified with CNTs (CNT-ME), or AgNPs (AgNP-ME) have been developed. Under optimized conditions, CNT-MEs showed better performance compared to AgNP-ME, yielding a linear range of detection over a dynamic concentration range of 1 fM–35 μM and 2 pM–200 nM, respectively, as well as high selectivity towards furaneol. Finally, our aptasensor was tested in a real sample (strawberry) and validated with high-performance liquid chromatography (HPLC), showing that it could find an application in the food industry.

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Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review

Cited by 109 publications

References 132 publications

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

An Overview on SARS-CoV-2 (COVID-19) and Other Human Coronaviruses and Their Detection Capability via Amplification Assay, Chemical Sensing, Biosensing, Immunosensing, and Clinical Assays

Surface Display Technology for Biosensor Applications: A Review

Flexible Screen Printed Aptasensor for Rapid Detection of Furaneol: A Comparison of CNTs and AgNPs Effect on Aptasensor Performance

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