Aptasensor Based on Hierarchical Core–Shell Nanocomposites of Zirconium Hexacyanoferrate Nanoparticles and Mesoporous mFe₃O₄@mC: Electrochemical Quantitation of Epithelial Tumor Marker Mucin-1

Abstract

A novel nanostructured hierarchical core–shell nanocomposite of zirconium hexacyanoferrate (ZrHCF) and a mesoporous nanomaterial composed of Fe3O4 and carbon nanospheres (denoted as ZrHCF@mFe3O4@mC) was prepared and used as a novel platform for an aptasensor to detect the epithelial tumor marker mucin-1 (MUC1) sensitively and selectively. The prepared ZrHCF@mFe3O4@mC nanocomposite exhibited good chemical functionality, water stability, and high specific surface area. Therefore, large amounts of aptamer molecules resulted in high sensitivity of the developed electrochemical aptasensor toward traces of MUC1. The constructed sensor also showed a good linear relationship with the logarithm of MUC1 concentration in the broad range of 0.01 ng·mL–1 to 1.0 μg·mL–1, with a low detection limit of 0.90 pg·mL–1. The fabricated ZrHCF@mFe3O4@mC-based aptasensor exhibited not only high selectivity because of the formation of aptamer–MUC1 complex but also good stability, acceptable reproducibility, and applicability. The proposed novel strategy based on a newly prepared hierarchical core–shell nanocomposite demonstrated outstanding biosensing performance and presents potential applications in biomedical fields.

Physicochemical Properties of Nanostructured Complexes Formed between Deferrioxamine Siderophore and Zr(IV), Hf(IV), or Fe(III) Metal Ions at the Gold Electrode/Solution Interface: A Comparative Study

“…The high-resolution spectrum obtained on the Au−MPA−DFO− Fe(III) (panel B) shows two characteristic peaks in the region of 705−735 eV related to Fe 3+ 2p 1/2 and Fe 3+ 2p 3/2 , which is interesting and further supports the above discussions, specially the EDX results. 72 The CV and EIS measurements performed in the presence of 2.0 mM FcMeOH redox probe (Figure S1) support the accumulation of Fe(III) by the Au−MPA−DFO system.…”

“…The XPS survey spectrum obtained on the Au–MPA–DFO–Fe­(III) system (panel A) indicates several peaks related to C 1s, S 2p, N 1s, O 1s, Au 4p, Au 4d, Au 4f, and Fe 2p. The high-resolution spectrum obtained on the Au–MPA–DFO–Fe­(III) (panel B) shows two characteristic peaks in the region of 705–735 eV related to Fe 3+ 2p 1/2 and Fe 3+ 2p 3/2 , which is interesting and further supports the above discussions, specially the EDX results …”

Surface polarization of BiOI to boost photoelectrochemical signal transduction for high-performance bioassays

“…Similarly, the cathodic photocurrent increased linearly with an increase in the MUC1 protein concentration in the range 1.0 fg mL −1 to 30.0 ng mL −1 (Fig. 2A and B), and a low detection limit of 0.3 fg mL −1 was achieved (S/N = 3), a value superior to those from previous assays for the MUC1 protein 24–29 (Table S4, ESI†). None of the tested interferents caused any obvious change in the signal, thereby confirming the satisfactory selectivity of the assay for the detection of the MUC1 protein (Fig.…”

Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review

“…In 2000, this group named PB an "artificial PO" [177]. At the same time, a large number of other scientific groups, especially from China, worked hard on this problem [148,154,[156][157][158][159]166,[168][169][170][171][172][173][174].…”

“…PB and its analogues (PBAs) are cheap, easy to synthesize, environmentally friendly and are prospect for wide applications in different fields, including basic research and industrial purposes [ 10 , 147 , 148 , 149 , 150 , 151 ] as well as in medicine [ 24 , 152 , 153 , 154 , 155 , 156 , 157 , 158 ].…”