Aptasensor based on fluorescence resonance energy transfer for the analysis of adenosine in urine samples of lung cancer patients

Hashemian, Zahra; Khayamian, Taghi; Saraji, Mohammad; Shirani, Marziyeh Poshteh

doi:10.1016/j.bios.2015.12.028

Cited by 45 publications

(13 citation statements)

References 41 publications

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“…To conjugate the CS and CDs covalently, CDs were reacted with CS using the standard EDC protocol . In brief, 60 μl CDs solution were diluted with 200 μl phosphate buffer (pH 7.1), and 8 μl of 50 mM EDC and 8 μl of 50 mM NHS were mixed together.…”

Section: Methodsmentioning

confidence: 99%

A fluorescent aptasensor for analysis of adenosine triphosphate based on aptamer–magnetic nanoparticles and its single‐stranded complementary DNA labeled carbon dots

2018

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A new fluorimetric aptasensor was designed for the determination of adenosine triphosphate (ATP) based on magnetic nanoparticles (MNPs) and carbon dots (CDs). In this analytical strategy, an ATP aptamer was conjugated on MNPs and a complementary strand of the aptamer (CS) was labeled with CDs. The aptamer and its CS were hybridized to form a double helical structure. The hybridized aptamers could be used for the specific recognition of ATP in a biological complex matrix using a strong magnetic field to remove the interfering effect. In the absence of ATP, no CDs-CS could be released into the solution and this resulted in a weak fluorescence signal. In the presence of ATP, the target binds to its aptamer and causes the dissociation of the double helical structure and liberation of the CS, such that a strong fluorescence signal was generated. The increased fluorescence signal was proportional to ATP concentration. The limit of detection was estimated to be 1.0 pmol L with a dynamic range of 3.0 pmol L to 5.0 nmol L . The specific aptasensor was applied to detect ATP in human serum samples with satisfactory results. Moreover, molecular dynamic simulation (MDS) studies were used to analyze interactions of the ATP molecule with the aptamer.

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Section: Methodsmentioning

confidence: 99%

A fluorescent aptasensor for analysis of adenosine triphosphate based on aptamer–magnetic nanoparticles and its single‐stranded complementary DNA labeled carbon dots

2018

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“…In a research, an aptasensor was designed to detect the cytochrome c 91 . This aptasensor was used to investigate the drug treatment in the liver cancer; the screen mechanism was related to diagnosis of cytochrome c; cytochrome c role in apoptosis as one of the fighter processes against cancer is clear, so that the B-cell lymphoma 2 (Bcl-2) regulatory protein is the cause in order to release the cytochrome c from the mitochondria, which leads to the activation of caspase-9 and then caspase-3; eventually, this pathway leads to cell apoptosis 92 .…”

Section: Other Applications Of Aptasensors In the Cancer Follow-upmentioning

confidence: 99%

Application of electrochemical aptasensors in detection of cancer biomarkers

Negahdary

Heli

2019

Biomed. Res. Ther.

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Today, the late diagnosis of cancers is a big challenge, and using novel diagnostic techniques will provide essential, faster and more accurate treatments. Unfortunately, existing common and traditional diagnostic methods have not been helpful completely and most cancers are diagnosed too late. Recently, researchers have found new diagnostic methods against cancers by aptasensors; these sensory systems can detect involved biomarkers in various cancers so that the research in this field is continued strictly. Aptasensors can detect cancer markers in small quantities and high selectivity; moreover, other advantages of cancer aptasensors such as optimized time and cost saving can be considered. In addition, the aptasensors have been used in the diagnosis of the effective and related factors in cancer therapy follow-up. Here, the most researches about cancer aptasensors and other involved markers were collected, reviewed and described.

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“…[32] Over the past several years,s tructurally switched responses from adenosine aptamers have been "interpreted"i nto colorimetric, [33] luminescent, [34] electrochemical, [35] electro-chemilumi-nescent, [36] ands urface-enhanced Ramans cattering (SERS) [37] signals in variousadenosine-detectiona ssays. [32] Over the past several years,s tructurally switched responses from adenosine aptamers have been "interpreted"i nto colorimetric, [33] luminescent, [34] electrochemical, [35] electro-chemilumi-nescent, [36] ands urface-enhanced Ramans cattering (SERS) [37] signals in variousadenosine-detectiona ssays.…”

Section: Detection Of Adenosinementioning

confidence: 99%

The Development of G‐Quadruplex‐Based Assays for the Detection of Small Molecules and Toxic Substances

Dong

et al. 2017

Chemistry An Asian Journal

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G-Quadruplexes can be induced to form guanine-rich DNA sequences by certain small molecules or metal ions. In concert with an appropriate signal transducer, such as a fluorescent dye or a phosphorescent metal complex, the ligand-recognition event can be transduced into a luminescent response. This focus review aims to highlight recent examples of aptamer-based and metal-mediated G-quadruplex assays for the detection of small molecules and toxic substances in the last three years. We discuss the mechanisms and features of the different assays and present an outlook and a perspective for the future of this field.

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Aptasensor based on fluorescence resonance energy transfer for the analysis of adenosine in urine samples of lung cancer patients

Cited by 45 publications

References 41 publications

A fluorescent aptasensor for analysis of adenosine triphosphate based on aptamer–magnetic nanoparticles and its single‐stranded complementary DNA labeled carbon dots

A fluorescent aptasensor for analysis of adenosine triphosphate based on aptamer–magnetic nanoparticles and its single‐stranded complementary DNA labeled carbon dots

Application of electrochemical aptasensors in detection of cancer biomarkers

The Development of G‐Quadruplex‐Based Assays for the Detection of Small Molecules and Toxic Substances

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