Aptamers to Nucleic Acid Structures

Toulmé, Jean‐Jacques; Darfeuille, Fabien; Primo, Carmelo Di; Dausse, Eric

doi:10.1002/3527608192.ch7

Cited by 4 publications

(3 citation statements)

References 66 publications

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“…In principle, there is no restriction in the type of target for which the aptamer can be selected. To date, aptamers with affinity to various ligands were synthesized, including metal ions, organic dyes, drugs, amino acids, cofactors [22], antibiotics [23] and nucleic acid structures [24]. Special interest was focused on the development of aptamers for the detection of proteins including enzymes, antibodies, prions, growth factors, gene regulatory factors, cell adhesion molecules and lectins.…”

Section: Structure and Molecular Engineering Of Aptamersmentioning

confidence: 99%

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Electrochemical Aptasensors – Recent Achievements and Perspectives

2009

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This article reviews recent achievements in developing aptamer-based electrochemical biosensors (electrochemical aptasensors). Aptamers are single stranded DNA or RNA molecules with high specificity to various ligands. Their specificity is comparable and in certain cases even higher than those of antibodies. In contrast to antibodies, aptamers are prepared by an in vitro selection procedure developed simultaneously in the early 1990s by L. Gold and A. Ellington. Due to their stability and the possibility of chemical modification aptamers can be immobilized on various supports and serve as artificial receptors in biosensors. The first aptasensors developed in the second half of 1990s were based on optical detection. However, in early 2000 substantial interest arose to the development of electrochemical aptasensors. It has been shown that due to their simplicity and fast response they represent an excellent tool in practical applications. The main focus of this review is to discuss the configuration of aptamers and electrochemical methods for detecting aptamers -analyte interactions. We will also provide a brief history of aptamer development, along with molecular structure and methods of aptamer engineering. Methods for immobilizing aptamers onto a solid support are also discussed.

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Section: Structure and Molecular Engineering Of Aptamersmentioning

confidence: 99%

“…The theoretical diversity of individual oligonucleotides in these random DNA libraries is rather large. For example, in the case of oligonucleotides composed of 40 bases, it is 4 40 ¼ 1.2 Â 10 24 . In practice, however, a considerably smaller library of approximately 10 13 -10 15 molecules is used [9].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensors – Recent Achievements and Perspectives

2009

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“…Oligonucleotides are poorly adapted to the recognition of RNA structures (Toulmé et al, 2005). Folded RNA regions are not available for intermolecular pairing with the complementary sequence.…”

Section: Aptaregulatorsmentioning

confidence: 99%