Aptamers: Potential Diagnostic and Therapeutic Agents for Blood Diseases

Aljohani, Maher M.; Cialla‐May, Dana; Popp, Jürgen; Chinnappan, Raja; Alkattan, Khaled; Zourob, Mohammed

doi:10.3390/molecules27020383

Cited by 37 publications

(27 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aptamers have been employed in biosensing and biomedical applications [ 43 , 44 ]. Regarding the latter, their use in medical imaging, diagnosis and treatment of diseases, targeted drug delivery, and regenerative medicine have already been described [ 44 , 45 , 46 , 47 , 48 ]. As far as biosensing applications are concerned, aptamers are used as recognition and binding elements.…”

Section: Surface-residing Molecular Targetsmentioning

confidence: 99%

Molecular Targets for Foodborne Pathogenic Bacteria Detection

Paramithiotis

2023

Pathogens

View full text Add to dashboard Cite

The detection of foodborne pathogenic bacteria currently relies on their ability to grow on chemically defined liquid and solid media, which is the essence of the classical microbiological approach. Such procedures are time-consuming and the quality of the result is affected by the selectivity of the media employed. Several alternative strategies based on the detection of molecular markers have been proposed. These markers may be cell constituents, may reside on the cell envelope or may be specific metabolites. Each marker provides specific advantages and, at the same time, suffers from specific limitations. The food matrix and chemical composition, as well as the accompanying microbiota, may also severely compromise detection. The aim of the present review article is to present and critically discuss all available information regarding the molecular targets that have been employed as markers for the detection of foodborne pathogens. Their strengths and limitations, as well as the proposed alleviation strategies, are presented, with particular emphasis on their applicability in real food systems and the challenges that are yet to be effectively addressed.

show abstract

Section: Surface-residing Molecular Targetsmentioning

confidence: 99%

Molecular Targets for Foodborne Pathogenic Bacteria Detection

Paramithiotis

2023

Pathogens

View full text Add to dashboard Cite

show abstract

“…High specificity for targets has made aptamers an attractive tool in biomedical applications like diagnostics, imaging, and therapeutics (Barbas et al, 2010; Liu et al, 2012). The prospects of aptamers for clinical application are recently reviewed by many authors (Aljohani et al, 2022; Devi et al, 2021; Liu et al, 2022). They are studied as delivery agents incorporated into constructs that can carry therapeutic payload like siRNA, chemotherapeutic agents, cell toxins, and nanoparticles (Barbas et al, 2010).…”

Section: Rna Interferencementioning

confidence: 99%

Aptamer‐siRNA chimeras: Promising tools for targeting HER2 signaling in cancer

Dhanya¹,

Mary

Madhavan³

2022

Chem Biol Drug Des

View full text Add to dashboard Cite

RNA interference is a transformative approach and has great potential in the development of novel and more efficient cancer therapeutics. Immense prospects exist in the silencing of HER2 and its downstream genes which are overexpressed in many cancers, through exogenously delivered siRNA. However, there is still a long way to exploit the full potential and versatility of siRNA therapeutics due to the challenges associated with the stability and delivery of siRNA targeted to specific sites. Aptamers offer several advantages as a vehicle for siRNA delivery, over other carriers such as antibodies. In this review, we discuss the progress made in the development and applications of aptamer‐siRNA chimeras in HER2 targeting and gene silencing. A schematic workflow is also provided which will provide ample insight for all those researchers who are new to this field. Also, we think that a mechanistic understanding of the HER2 signaling pathway is crucial in designing extensive investigations aimed at the silencing of a wider array of genes. This review is expected to stimulate more research on aptamer‐siRNA chimeras targeted against HER2 which might arm us with potential effective therapeutic interventions for the management of cancer.

show abstract

“…Several aspects of aptamers are superior to antibodies; for example, aptamers are easier to obtain, are less expensive to produce, and exhibit better biocompatibility (Byun 2021). Aptamers are widely used in various biomedical elds, including therapeutics, biosensors, diagnostics, drug delivery and imaging systems (Odeh et al 2019;Aljohani et al 2022). However, aptamers are susceptible to degradation by nucleases, which severely limits their application in biological uids (Daniel et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Optimizing the expression and characterization of a thermostable ssDNA ligase from a Thermus scotoductus bacteriophage TS2126

Ma¹,

Chen²,

Yang³

et al. 2023

Preprint

View full text Add to dashboard Cite

Although aptamers are widely used in various biomedical areas as novel molecular recognition elements, they are degraded by nucleases in biological fluids due to short single-stranded DNA (ssDNA) or RNA oligonucleotides. Research shows that this problem can be solved by circularizing aptamers with circular ligases, which can catalyze the intramolecular ligation of ssDNA templates with 5'-phosphate and 3'-hydroxyl groups. Herein, a thermostable ssDNA ligase was successfully expressed and purified for subsequent aptamer cyclization. The purified ligase showed good circularization activity for substrates with different lengths and had much higher circularization efficiency than T4 RNA ligase 1. Moreover, circularization reaction conditions, such as the concentration of MnCl2, enzyme and betaine, were studied to ascertain the optimal conditions for the catalytic reaction. The results indicated that the highest ligating activity occurred at MnCl2, enzyme and betaine concentrations of 1.25–2.5 mM, 0.02 mM and 1 М, respectively. Based on these, templates with different terminal nucleotides were tested to assess the sequence dependence of ligase. The results show that the efficiency of circularization is sequence dependent, in which 5'-G and 3'-T are the terminal nucleotides most favored by ligase. Finally, the stability asssy further confirmed that the circular aptamer was more stable than its linear form in biological fluids.

show abstract

Aptamers: Potential Diagnostic and Therapeutic Agents for Blood Diseases

Cited by 37 publications

References 115 publications

Molecular Targets for Foodborne Pathogenic Bacteria Detection

Molecular Targets for Foodborne Pathogenic Bacteria Detection

Aptamer‐siRNA chimeras: Promising tools for targeting HER2 signaling in cancer

Optimizing the expression and characterization of a thermostable ssDNA ligase from a Thermus scotoductus bacteriophage TS2126

Contact Info

Product

Resources

About