Aptamers improve the expression of a human granulocyte-macrophage colony stimulating factor in transgenicArabidopsis thaliana seeds

Abstract

Domain IV nucleotides of human 7SL RNA comprise an approximately 50-nucleotide region with a highly conserved secondary structure consisting of two internal loops. We inserted these into a modified 3'-untranslated region of the human granulocyte-macrophage colony stimulating factor (hGM-CSF) gene. The expression vector, under the control of a tissue-specific promoter derived from the soybean ~' subunit of ~3-conglycinin, was introduced into Arabidopsis thaliana by Agrobacteriurn.

Seeds as a Production System for Molecular Pharming Applications: Status and Prospects

“…Soybean seeds [83][84][85][86] have also been used to produce primarily vaccine candidates, including the heat-labile enterotoxin B subunit (LTB) vaccine [83], a nucleocapsid protein from Porcine reproductive and respiratory syndrome virus (PRRSV) [85], and full length humanized IgG recognizing herpes simplex virus 2 (HSV-2) [27]. Like tobacco, Arabidopsis seeds have been used for mainly experimental products [87][88][89][90][91][92][93][94], whereas wheat has been pioneered not only for the production of antibodies, but also heterologous seed storage proteins and the nutrient enhancer phytase [54,95,96]. Safflower seeds have been used to produce apolipoprotein [97], hirudin [98] and human insulin [99] and have been developed as an alternative to canola/rapeseed [87], [99].…”

“…Human enkephalins Antihyperanalgesic peptide [87] 42 k-Da fragment of P.falciparum merozoite surface protein 1 Vaccine antigen [88] Recombinant antibody 2G12 (anti-HIV monoclonal antibody) Antibody [89] HA78 against Hepatitis A virus Antibody [89] Insulin Hormone [90], [30] Carcinoembryonic antigen scFv (CEA) Antigen [91] Human granulocyte-macrophage colony stimulating factor Hormone [92] Cellulose Industrial enzyme [93] Arabidopsis Human -L-iduronidase Enzyme [94] Carcinoembryonic antigen scFv (CEA) Antigen [54] Pea the appropriate promoters and targeting sequences, increasing protein yields by providing an ideal stable environment [113], [114]. Most cereals form a single type of protein body, whereas rice endosperm forms two distinct types of compartment, known as PB-I and PB-II [69].…”