Aptamers-functionalized nanoscale MOFs for saxitoxin and tetrodotoxin sensing in sea foods through FRET

Dou, Xilin; Xu, Shihan; Jiang, Yifei; Ding, Zhaoyang; Xie, Jing

doi:10.1016/j.saa.2022.121827

Cited by 20 publications

(2 citation statements)

References 36 publications

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“…The change in the fluorescence lifetime indicated that the enhanced fluorescence between Ce-MOF and oxTMB was based on the shortened fluorescence lifetime of Ce-MOF as an energy donor by FRET [ 42 ]. In addition, with the increase in the concentration of Na 2 S, the oxTMB in the solution was reduced to TMB, the oxTMB decreased, and the fluorescence emission intensity of the detection system at 410 nm gradually increased, indicating that the FRET effect was enhanced [ 43 , 44 ].…”

Section: Resultsmentioning

confidence: 99%

Dual-Mode Ce-MOF Nanozymes for Rapid and Selective Detection of Hydrogen Sulfide in Aquatic Products

Cheng,

Du,

et al. 2024

Polymers

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Increasing concern over the safety of consumable products, particularly aquatic products, due to freshness issues, has become a pressing issue. Therefore, ensuring the quality and safety of aquatic products is paramount. To address this, a dual-mode colorimetric–fluorescence sensor utilizing Ce-MOF as a mimic peroxidase to detect H2S was developed. Ce-MOF was prepared by a conventional solvothermal synthesis method. Ce-MOF catalyzed the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) to produce blue oxidized TMB (oxTMB). When dissolved, hydrogen sulfide (H2S) was present in the solution, and it inhibited the catalytic effect of Ce-MOF and caused the color of the solution to fade from blue to colorless. This change provided an intuitive indication for the detection of H2S. Through steady-state dynamic analysis, the working mechanism of this sensor was elucidated. The sensor exhibited pronounced color changes from blue to colorless, accompanied by a shift in fluorescence from none to light blue. Additionally, UV–vis absorption demonstrated a linear correlation with the H2S concentration, ranging from 200 to 2300 µM, with high sensitivity (limit of detection, LOD = 0.262 μM). Fluorescence intensity also showed a linear correlation, ranging from 16 to 320 µM, with high selectivity and sensitivity (LOD = 0.156 μM). These results underscore the sensor’s effectiveness in detecting H2S. Furthermore, the sensor enhanced the accuracy of H2S detection and fulfilled the requirements for assessing food freshness and safety.

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Section: Resultsmentioning

confidence: 99%

Dual-Mode Ce-MOF Nanozymes for Rapid and Selective Detection of Hydrogen Sulfide in Aquatic Products

Cheng,

Du,

et al. 2024

Polymers

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“…The same strategy was recently applied to pufferfish samples [114]. Dou and colleagues designed an assay based on the fluorescence resonance energy transfer (FRET) phenomena to detect TTXs in shellfish samples [115]. Fluorescent nanoscale metal-organic frameworks (NMOFs) were used as an energy donor.…”

Section: Aptamer-based Biosensorsmentioning

confidence: 99%

Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems

Reverté

Alkassar

Diogène

et al. 2023

Foods

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The emergence of marine toxins such as ciguatoxins (CTXs) and tetrodotoxins (TTXs) in non-endemic regions may pose a serious food safety threat and public health concern if proper control measures are not applied. This article provides an overview of the main biorecognition molecules used for the detection of CTXs and TTXs and the different assay configurations and transduction strategies explored in the development of biosensors and other biotechnological tools for these marine toxins. The advantages and limitations of the systems based on cells, receptors, antibodies, and aptamers are described, and new challenges in marine toxin detection are identified. The validation of these smart bioanalytical systems through analysis of samples and comparison with other techniques is also rationally discussed. These tools have already been demonstrated to be useful in the detection and quantification of CTXs and TTXs, and are, therefore, highly promising for their implementation in research activities and monitoring programs.

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A label-free ratiometric fluorescent aptasensor based on a peroxidase-mimetic multifunctional ZrFe-MOF for the determination of tetrodotoxin

Liu,

Huo,

et al. 2023

Microchim Acta

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Aptamers-functionalized nanoscale MOFs for saxitoxin and tetrodotoxin sensing in sea foods through FRET

Cited by 20 publications

References 36 publications

Dual-Mode Ce-MOF Nanozymes for Rapid and Selective Detection of Hydrogen Sulfide in Aquatic Products

Dual-Mode Ce-MOF Nanozymes for Rapid and Selective Detection of Hydrogen Sulfide in Aquatic Products

Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems

A label-free ratiometric fluorescent aptasensor based on a peroxidase-mimetic multifunctional ZrFe-MOF for the determination of tetrodotoxin

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