Aptamers functionalized metal and metal oxide nanoparticles: Recent advances in heavy metal monitoring

Sawan, Simona; Errachid, Abdelhamid; Maalouf, Rita; Jaffrezic‐Renault, Nicole

doi:10.1016/j.trac.2022.116748

Cited by 20 publications

(11 citation statements)

References 78 publications

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“…AuNPs can also be combined with biological molecules (e.g. DNA) for applications in biomedical and bioanalytical devices [33] , [34] , [35] , [36] , [37] , [38] . For instance, it is possible to add ligands onto the surface of the AuNPs, allowing specific links with biomarkers of interest, increasing sensor selectivity [39] , [40] , [41] .…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles

Catunda

Prado

Oliveira

et al. 2023

Electrochimica Acta

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Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles

Catunda

Prado

Oliveira

et al. 2023

Electrochimica Acta

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“…Aptamers are oligonucleotides obtained by the systematic evolution of ligands by exponential enrichment (SELEX) that can bind to a variety of target molecules, such as toxins, , antibiotics, , molecular markers, , heavy metals, , proteins, − and cells , with high affinity and specificity. Aptamers have the characteristics of simple synthesis, convenient chemical modification, high thermal stability, almost no batch-to-batch differences, and easy storage and transportation. − They are primarily present in the form of RNA and ssDNA .…”

mentioning

confidence: 99%

FAM Tag Size Separation-Based Capture-Systematic Evolution of Ligands by Exponential Enrichment for Sterigmatocystin-Binding Aptamers with High Specificity

Zheng,

Ye,

Liu

et al. 2024

Anal. Chem.

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Sterigmatocystin (ST) is a known toxin whose aptamer has rarely been reported because ST is a water-insoluble small-molecule target with few active sites, leading to difficulty in obtaining its aptamer using traditional target fixation screening methods. To obtain aptamer for ST, we incorporated FAM tag size separation into the capture-systematic evolution of ligands by exponential enrichment and combined it with molecular activation for aptamer screening. The screening process was monitored using a quantitative polymerase chain reaction fluorescence amplification curve and recovery of negative-, counter-, and positiveselected ssDNA. The affinity and specificity of the aptamer were verified by constructing an aptamer-affinity column, and the binding sites were predicted using molecular docking simulations. The results showed that the K d value of the H Seq02 aptamer was 25.3 nM. The aptamer-affinity column based on 2.3 nmol of H Seq02 exhibited a capacity of about 80 ng, demonstrating better specificity than commercially available antibody affinity columns. Molecular simulation docking predicted the binding sites for H Seq02 and ST, further explaining the improved specificity. In addition, circular dichroism and isothermal titration calorimetry were used to verify the interaction between the aptamer and target ST. This study lays the foundation for the development of a new ST detection method.

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“…Pathological examination, sometimes known as the "gold standard" for breast cancer diagnosis, can diagnose the disease scientifically and accurately. 1,2 However, it has the disadvantages of being time-consuming and invasive, which is not conducive to early cancer diagnosis. As a noninvasive detection tool, liquid biopsy has a promising future in the early diagnosis of cancers.…”

mentioning

confidence: 99%

“…Breast cancer is one of the most dangerous cancers affecting women’s health and lives. Pathological examination, sometimes known as the “gold standard” for breast cancer diagnosis, can diagnose the disease scientifically and accurately. , However, it has the disadvantages of being time-consuming and invasive, which is not conducive to early cancer diagnosis. As a noninvasive detection tool, liquid biopsy has a promising future in the early diagnosis of cancers. − Exosomes are extracellular vesicles with a diameter of around 30–150 nm and are ideal biomarkers for liquid biopsy, as they carry a variety of bioactive molecules from their parent cells like DNA, RNA, proteins, and so on. − The identification of tumor-related proteins on exosomes can be exploited for early tumor diagnosis, which is especially important for breast cancer detection.…”

mentioning

confidence: 99%

Dual-Aptamer-Assisted Ratiometric SERS Biosensor for Ultrasensitive and Precise Identification of Breast Cancer Exosomes

Zhang

et al. 2023

ACS Sens.

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Due to the heterogeneity of breast cancer, its early accurate diagnosis remains a challenge. Exosomes carry abundant genetic materials and proteins and are ideal biomarkers for early cancer detection. Herein, a ratiometric surface-enhanced Raman scattering (SERS) biosensor for exosome detection was constructed using a regularly arranged Au@Ag nanoparticles/graphene oxide (Au@Ag NPs/GO) substrate with 4nitrothiophenol (4-NTP) molecules as an internal standard. Aptamers of two overexpressed proteins (epithelial cell adhesion molecule and human epidermal growth factor receptor 2) were linked by a short complementary DNA with rhodamine X modified at the 3′-terminal to form V-shaped double-stranded DNA, which attached to the surface of Au@Ag NPs/GO substrate for the selective recognition of breast cancer cell-derived exosomes. In the presence of exosomes, a competitive reaction occurred, resulting in the formation of the V-shaped double-stranded DNA/exosomes complex, and the V-shaped double-stranded DNA separated from the SERS substrate. The SERS signal of rhodamine X on the V-shaped double-stranded DNA decreased with the concentration of exosomes increasing, whereas the SERS signal of 4-NTP on the substrate remained stable. The ratiometric SERS strategy provides huge electromagnetic enhancement and abundant DNA adsorbing sites on the GO layer, achieving a wide detection range of 2.7 × 10 2 to 2.7 × 10 8 particles/mL and an ultralow limit of detection down to 1.5 × 10 2 particles/mL, without the requirement of any nucleic acid amplification. Particularly, the proposed method has significant applications in early cancer diagnosis as it can accurately identify breast cancer cell-derived exosomes in clinical serum samples and can differentiate pancreatic cancer patients and healthy individuals.

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Aptamers functionalized metal and metal oxide nanoparticles: Recent advances in heavy metal monitoring

Cited by 20 publications

References 78 publications

SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles

SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles

FAM Tag Size Separation-Based Capture-Systematic Evolution of Ligands by Exponential Enrichment for Sterigmatocystin-Binding Aptamers with High Specificity

Dual-Aptamer-Assisted Ratiometric SERS Biosensor for Ultrasensitive and Precise Identification of Breast Cancer Exosomes

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