Aptamers dimerization inspired biomimetic clamp assay towards impedimetric SARS-CoV-2 antigen detection

Han, Cong; Xing, Wenping; Li, Wenjin; Fang, Xiaona; Zhao, Jian; Ge, Feng; Ding, Wei; Qu, Pengpeng; Z, Luo; Zhang, Liyun

doi:10.1016/j.snb.2023.133387

Cited by 10 publications

(4 citation statements)

References 38 publications

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“…This could cause false positives or interfere with biosensing. 162 Especially in the medical and diagnostic fields, obtaining regulatory permission for biosensors based on aptamers or biomimetic receptors can be a time-consuming and difficult procedure. 163 It can be difficult to include aptamers and biomimetic receptors into useful biosensing devices that have automated sample processing, userfriendly interfaces, and reliable performance in real-world settings.…”

Section: Applications Of Aptamers and Biomimetic Receptors In Biosensingmentioning

confidence: 99%

Review—Aptamers and Biomimetic Receptors in Biosensing: Innovations and Applications

Tripathi,

Pandey,

Mishra

et al. 2024

J. Electrochem. Soc.

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The fields of biosensing have been transformed by the discovery of extraordinary molecular recognition components, such as aptamers and biomimetic receptors. Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is a method used to select aptamers, or short sequences of single-stranded DNA (ssDNA) or RNA (ssRNA), based on their unique binding affinity to target molecules. Molecularly imprinted polymers (MIPs) are a type of biomimetic receptor that mimics the selectivity of natural receptors inside a synthetic matrix. They make it possible to identify pathogens, and illness biomarkers with accuracy. Aptamers and biomimetic receptors play crucial roles in various fields including diagnostics, therapeutics, and biosensing. Their high specificity, versatility, and adaptability enable targeted detection, drug delivery, and biomolecule manipulation, thereby contributing to advancements in personalized medicine, biotechnology, and disease diagnosis. Aptamers and biomimetic receptors have been combined with cutting-edge technologies, like nanotechnology and lab-on-a-chip systems, to create biosensors that are quick, portable, and extremely sensitive. These recognition features are anticipated to become more important as technology develops, helping to address global issues, advance biosensing capabilities, and raise people's standard of living everywhere. Recent advancements and innovation on Aptamers and Biomimetic Receptors in Biosensing have been discussed in this review article.

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Section: Applications Of Aptamers and Biomimetic Receptors In Biosensingmentioning

confidence: 99%

Review—Aptamers and Biomimetic Receptors in Biosensing: Innovations and Applications

Tripathi,

Pandey,

Mishra

et al. 2024

J. Electrochem. Soc.

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“…In addition, the aptamer was immobilized on AuNPs on the carbon electrode to improve the detection time of SARS-CoV-2, and the electrochemical impedance was analyzed after incubation for 40 min at various target concentrations, resulting in an LOD of 66 pg/mL for SARS-CoV-2 RBD [ 107 ]. Furthermore, Han et al [ 108 ] reported aptamer dimerization inspired by the biomimetic clamp assay for SARS-CoV-2 detection, in which a pair of sandwich-type aptamers are dimerized to achieve high-performance target recognition. They incorporated the DNA biomimetic clamp (DNA-BC) into AuNP-decorated amino-functionalized UiO-66 (Au@UiO-66-NH 2 )-modified screen-printed gold electrodes (SPGEs) to form adsorption sites and enhance the electrical conductivity.…”

Section: Aptamersmentioning

confidence: 99%

Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications

Park,

Lee

et al. 2024

Biosensors

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The global challenges posed by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic have underscored the critical importance of innovative and efficient control systems for addressing future pandemics. The most effective way to control the pandemic is to rapidly suppress the spread of the virus through early detection using a rapid, accurate, and easy-to-use diagnostic platform. In biosensors that use bioprobes, the binding affinity of molecular recognition elements (MREs) is the primary factor determining the dynamic range of the sensing platform. Furthermore, the sensitivity relies mainly on bioprobe quality with sufficient functionality. This comprehensive review investigates aptamers and nanobodies recently developed as advanced MREs for SARS-CoV-2 diagnostic and therapeutic applications. These bioprobes might be integrated into organic bioelectronic materials and devices, with promising enhanced sensitivity and specificity. This review offers valuable insights into advancing biosensing technologies for infectious disease diagnosis and treatment using aptamers and nanobodies as new bioprobes.

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“…In addition to polymers, dendrimers, and SAMs, we can highlight the use of metalorganic frameworks (MOFs) [132][133][134][135], as well as metal complexes of porphyrins [136] and phthalocyanines [137,138].…”

Section: Metal Complexes Of Porphyrins Phthalocyanines and Metal-orga...mentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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Aptamers dimerization inspired biomimetic clamp assay towards impedimetric SARS-CoV-2 antigen detection

Cited by 10 publications

References 38 publications

Review—Aptamers and Biomimetic Receptors in Biosensing: Innovations and Applications

Review—Aptamers and Biomimetic Receptors in Biosensing: Innovations and Applications

Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

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