Aptamer-quantum dots and teicoplanin-gold nanoparticles constructed FRET sensor for sensitive detection of Staphylococcus aureus

Tao, Xiaoqi; Liao, Ziyi; Zhang, Yaqing; Fu, Fei; Hao, Mengqi; Song, Yang; Song, Erqun

doi:10.1016/j.cclet.2020.07.020

Cited by 53 publications

(27 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method achieved an LOD of 2 CFU mL −1 . 190 In addition, chimeric bacteriophage is another potential candidate. Thanks to the developments on phage display technique, Chen's group have introduced a set of engineered M13 phages displaying the receptor-binding protein from a phage naturally targeting several bacteria, including Pseudomonas aeruginosa (P. aeruginosa) , Vibrio cholerae (V. cholerae) , and E. coli.…”

Section: Aunps-based Optical Nanosensors For Food and Health Safety M...mentioning

confidence: 99%

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Aunps-based Optical Nanosensors For Food and Health Safety M...mentioning

confidence: 99%

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

et al. 2022

View full text Add to dashboard Cite

show abstract

“…However, the use of traditional organic fluorescent dyes in sensors is limited due to low fluorescence intensity and rapid photobleaching. Compared with traditional organic fluorescent dyes, fluorescent nanomaterials such as quantum dots (QDs) ( Fu et al, 2020 ; Tao et al, 2021 ), upconversion nanoparticles (UCNPs) ( Wu et al, 2014 ; Ouyang et al, 2021b ) and carbon dots ( Cui et al, 2019 ; Pebdeni et al, 2020 ) that have emerged in recent years have many advantages, such as wider absorption spectra, size-tunable narrower emission spectra and better stability ( Yao et al, 2014 ).…”

Section: Aptasensors Based On Optical Transductionmentioning

confidence: 99%

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Chen

Lai

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The infection of Staphylococcus aureus (S.aureus) and the spread of drug-resistant bacteria pose a serious threat to global public health. Therefore, timely, rapid and accurate detection of S. aureus is of great significance for food safety, environmental monitoring, clinical diagnosis and treatment, and prevention of drug-resistant bacteria dissemination. Traditional S. aureus detection methods such as culture identification, ELISA, PCR, MALDI-TOF-MS and sequencing, etc., have good sensitivity and specificity, but they are complex to operate, requiring professionals and expensive and complex machines. Therefore, it is still challenging to develop a fast, simple, low-cost, specific and sensitive S. aureus detection method. Recent studies have demonstrated that fast, specific, low-cost, low sample volume, automated, and portable aptasensors have been widely used for S. aureus detection and have been proposed as the most attractive alternatives to their traditional detection methods. In this review, recent advances of aptasensors based on different transducer (optical and electrochemical) for S. aureus detection have been discussed in details. Furthermore, the applications of aptasensors in point-of-care testing (POCT) have also been discussed. More and more aptasensors are combined with nanomaterials as efficient transducers and amplifiers, which appears to be the development trend in aptasensors. Finally, some significant challenges for the development and application of aptasensors are outlined.

show abstract

“…This system can recognize and imaging prostate cancer cells that express prostate specific membrane antigen (PSMA) protein through delivery of Dox via activation of fluorescence QD. Recently, FRET aptasensors synthesized by conjugating Apt–QDs–AuNPs have been used for the detection of Staphylococcus aureus [ 60 ] and Tumor Necrosis Factor-alpha (TNF-α) [ 61 ]. The authors observed that selective binding occurred with limit of detection of 2 cfu/mL for S. aureus and strong fluorescence of an aptamer–QD conjugated donor was successfully quenched by an AuNPs acceptor in the presence of target molecules.…”

Section: Nanomaterials Based Aptamer Sensors As Diagnostic Toolmentioning

confidence: 99%

Recent Advances in Nanomaterial-Based Aptasensors in Medical Diagnosis and Therapy

et al. 2021

View full text Add to dashboard Cite

Rapid and accurate diagnosis of various biomarkers associated with medical conditions including early detection of viruses and bacteria with highly sensitive biosensors is currently a research priority. Aptamer is a chemically derived recognition molecule capable of detecting and binding small molecules with high specificity and its fast preparation time, cost effectiveness, ease of modification, stability at high temperature and pH are some of the advantages it has over traditional detection methods such as High Performance Liquid Chromatography (HPLC), Enzyme-linked Immunosorbent Assay (ELISA), Polymerase Chain Reaction (PCR). Higher sensitivity and selectivity can further be achieved via coupling of aptamers with nanomaterials and these conjugates called “aptasensors” are receiving greater attention in early diagnosis and therapy. This review will highlight the selection protocol of aptamers based on Traditional Systematic Evolution of Ligands by EXponential enrichment (SELEX) and the various types of modified SELEX. We further identify both the advantages and drawbacks associated with the modified version of SELEX. Furthermore, we describe the current advances in aptasensor development and the quality of signal types, which are dependent on surface area and other specific properties of the selected nanomaterials, are also reviewed.

show abstract

Aptamer-quantum dots and teicoplanin-gold nanoparticles constructed FRET sensor for sensitive detection of Staphylococcus aureus

Cited by 53 publications

References 38 publications

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Recent Advances in Nanomaterial-Based Aptasensors in Medical Diagnosis and Therapy

Contact Info

Product

Resources

About