Aptamer-mediated ‘turn-off/turn-on’ nanozyme activity of gold nanoparticles for kanamycin detection

Sharma, Tarun; Ramanathan, Rajesh; Weerathunge, Pabudi; Mohammadtaheri, Mahsa; Daima, Hemant Kumar; Shukla, Ravi; Bansal, Vipul

doi:10.1039/c4cc07275h

Cited by 207 publications

(120 citation statements)

References 34 publications

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“…21 We recently demonstrated that natural amino acids such as tyrosine can be employed as unique reducing agents for the synthesis of charge-switchable metal nanoparticles. 24,25 In our recent work, the control of nanoparticle surface corona through this strategy allowed us to efficiently bind bulky clusters of polyoxometalates on the surface of tyrosine-reduced Au and Ag nanoparticles, which provided bacterial targeting attributes to these nanomaterials without causing toxicity to mammalian cells. This unique property of amino acids provides a facile handle to control the nanosurface chemistry to facilitate the binding of a variety of molecules through electrostatic interactions.…”

Section: Introductionmentioning

confidence: 99%

Charge-switchable gold nanoparticles for enhanced enzymatic thermostability

Shankar

Soni

Daima

et al. 2015

Phys. Chem. Chem. Phys.

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This study illustrates a facile strategy for efficient immobilization of enzymes on a metal nanoparticle surface. The strategy proposed here enables the enzymatic activity to be retained while increasing the enzyme thermostability. It is demonstrated that the use of a zwitterionic amino acid tyrosine as a reducing and capping agent to synthesise gold nanoparticles allows efficient immobilization of phytase enzyme through charge-switchable electrostatic interactions. The detailed kinetic and thermodynamic studies reveal that the proposed enzyme immobilization strategy improves the overall quality of phytase by reducing the activation energy required for substrate hydrolysis and broadening the temperature window in which immobilized enzyme is able to operate. The outcomes of this study indicate that the underlying zwitterionic nature of 20 natural amino acids along with significant variability in their isoelectric points and hydropathy indices as well the ability of some of the amino acids to reduce metal ions is likely to offer significant opportunities for tailoring nano-bio interfaces in a rational manner for a range of biological applications.

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Section: Introductionmentioning

confidence: 99%

Charge-switchable gold nanoparticles for enhanced enzymatic thermostability

Shankar

Soni

Daima

et al. 2015

Phys. Chem. Chem. Phys.

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“…Besides the above tagging strategies, nanozyme tags are also often used in other immunoassays or immuno‐like assays (Das, Dhiman, Kapil, Bansal, & Sharma, ; Sharma et al., ). For example, Wu, Duan, Qiu, Li, and Wang () reported a colorimetric aptasensor platform for detecting Salmonella enterica serovar typhimurium ( S. typhimurium ) using the POD‐like ZnFe 2 O 4 ‐reduced graphene oxide nanostructures (ZnFe 2 O 4 /rGO) as a signal tag.…”

Section: Principles Of Detection Using Enzyme‐mimetic Nanomaterialsmentioning

confidence: 99%

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Huang

Sun

et al. 2019

Comp Rev Food Sci Food Safe

139

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The public concerns about agrifood safety call for innovative and reformative analytical techniques to meet the inspection requirements of high sensitivity, specificity, and reproducibility. Enzyme‐mimetic nanomaterials or nanozymes, which combine enzyme‐like properties with nanoscale features, emerge as an excellent tool for quality and safety detection in the agrifood sector, due to not only their robust capacity in detection but also their attraction in future‐oriented exploitations. However, in‐depth understanding about the fundamental principles of nanozymes for food quality and safety detection remains limited, which makes their applications largely empirical. This review provides a comprehensive overview of the principles, designs, and applications of nanozyme‐based detection technique in the agrifood industry. The discussion mainly involves three mimicking types, that is, peroxidase, oxidase, and catalase‐like nanozymes, capable of detecting major agrifood analytes. The current principles and strategies are classified and then discussed in details through discriminating the roles of nanozymes in diverse detection platforms. Thereafter, recent applications of nanozymes in detecting various endogenous ingredients and exogenous contaminants in foods are reviewed, and the outlook of profound developments are explained. Evidenced by the increasing publications, nanozyme‐based detection techniques are narrowing the gap to practical‐oriented food analytical methods, while some challenges in optimization of nanozymes, diversification of recognition‐to‐signal manners, and sustainability of methodology need to conquer in the future.

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“…Otherwise, nanozymes might change their original biocompatibility and these shielding compounds could be another toxic source. Once the surface protection is reversible, it is difficult to achieve in living systems due to easy formation of protein coronas around nanomaterials . Therefore, nanozymes urgently await the development of a fundamental approach for inhibiting their intrinsic peroxidase‐like activity in order to diminish their cytotoxicity while also retaining surface accessibility for other enzyme‐like activities.…”

Section: Introductionmentioning

confidence: 99%

Tailoring Enzyme‐Like Activities of Gold Nanoclusters by Polymeric Tertiary Amines for Protecting Neurons Against Oxidative Stress

Liu

Lin

et al. 2016

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The cytotoxicity of nanozymes has drawn much attention recently because their peroxidase-like activity can decompose hydrogen peroxide (H2 O2 ) to produce highly toxic hydroxyl radicals (•OH) under acidic conditions. Although catalytic activities of nanozymes are highly associated with their surface properties, little is known about the mechanism underlying the surface coating-mediated enzyme-like activities. Herein, it is reported for the first time that amine-terminated PAMAM dendrimer-entrapped gold nanoclusters (AuNCs-NH2 ) unexpectedly lose their peroxidase-like activity while still retaining their catalase-like activity in physiological conditions. Surprisingly, the methylated form of AuNCs-NH2 (i.e., MAuNCs-N(+) R3 , where R = H or CH3 ) results in a dramatic recovery of the intrinsic peroxidase-like activity while blocking most primary and tertiary amines (1°- and 3°-amines) of dendrimers to form quaternary ammonium ions (4°-amines). However, the hidden peroxidase-like activity is also found in hydroxyl-terminated dendrimer-encapsulated AuNCs (AuNCs-OH, inside backbone with 3°-amines), indicating that 3°-amines are dominant in mediating the peroxidase-like activity. The possible mechanism is further confirmed that the enrichment of polymeric 3°-amines on the surface of dendrimer-encapsulated AuNCs provides sufficient suppression of the critical mediator •OH for the peroxidase-like activity. Finally, it is demonstrated that AuNCs-NH2 with diminished cytotoxicity have great potential for use in primary neuronal protection against oxidative damage.

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Aptamer-mediated ‘turn-off/turn-on’ nanozyme activity of gold nanoparticles for kanamycin detection

Cited by 207 publications

References 34 publications

Charge-switchable gold nanoparticles for enhanced enzymatic thermostability

Charge-switchable gold nanoparticles for enhanced enzymatic thermostability

Development of Nanozymes for Food Quality and Safety Detection: Principles and Recent Applications

Tailoring Enzyme‐Like Activities of Gold Nanoclusters by Polymeric Tertiary Amines for Protecting Neurons Against Oxidative Stress

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