Aptamer‐Mediated Efficient Capture and Release of T Lymphocytes on Nanostructured Surfaces

Chen, Li; Liu, Xueli; Su, Bin; Li, Jing; Jiang, Lei; Han, Dong; Wang, Shutao

doi:10.1002/adma.201102435

Cited by 177 publications

(161 citation statements)

References 46 publications

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“…Although not all of the captured cells were released with the current device, the ability to release cells minimally invasively (8,15,41) represents a significant step toward studying the biology of these cells or screening for new drugs. One could envision that by encoding restriction sites for different restriction enzymes in the RCA product, one could release captured cells in a selective manner.…”

Section: Discussionmentioning

confidence: 99%

Bioinspired multivalent DNA network for capture and release of cells

Zhao

Cui

Bose

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

262

263

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Capture and isolation of flowing cells and particulates from body fluids has enormous implications in diagnosis, monitoring, and drug testing, yet monovalent adhesion molecules used for this purpose result in inefficient cell capture and difficulty in retrieving the captured cells. Inspired by marine creatures that present long tentacles containing multiple adhesive domains to effectively capture flowing food particulates, we developed a platform approach to capture and isolate cells using a 3D DNA network comprising repeating adhesive aptamer domains that extend over tens of micrometers into the solution. The DNA network was synthesized from a microfluidic surface by rolling circle amplification where critical parameters, including DNA graft density, length, and sequence, could readily be tailored. Using an aptamer that binds to protein tyrosine kinase-7 (PTK7) that is overexpressed on many human cancer cells, we demonstrate that the 3D DNA network significantly enhances the capture efficiency of lymphoblast CCRF-CEM cells over monovalent aptamers and antibodies, yet maintains a high purity of the captured cells. When incorporated in a herringbone microfluidic device, the 3D DNA network not only possessed significantly higher capture efficiency than monovalent aptamers and antibodies, but also outperformed previously reported cell-capture microfluidic devices at high flow rates. This work suggests that 3D DNA networks may have broad implications for detection and isolation of cells and other bioparticles.

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Section: Discussionmentioning

confidence: 99%

Bioinspired multivalent DNA network for capture and release of cells

Zhao

Cui

Bose

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

262

263

View full text Add to dashboard Cite

show abstract

“…To realize such control, different functional substrates have been developed, where cell adhesion can be controlled in response to external stimuli, such as heat [9,10], chemicals [11], light [12][13][14], potential and enzymatic activities [15,16], etc. However, most of the stimuli-responsive substrate focuses on the single response till now, which would not satisfy the needs of double or multiple stimulation in future applications.…”

Section: Introductionmentioning

confidence: 99%

Electrochemically and DNA-triggered cell release from ferrocene/β-cyclodextrin and aptamer modified dualfunctionalized graphene substrate

Feng

Ren

et al. 2014

Nano Res.

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Here we report a dual-functionalized electrochemical substrate to trigger cancer cells release based on the supramolecular interaction between β-cyclodextrin (β-CD) and Fc on clinical trial II aptamer AS1411 functionalized graphene platform. On one hand, the host-guest interaction can be reversible electrochemically controlled to realize cancer cells capture/release, and 1-adamantylamine binding can further amplify this surface change by competing interaction with β-CD. On the other hand, the AS1411 aptamer and its complementary DNA (cDNA) also can be used as a switchable anchor for cell adhesion. Our work gives an example for label-free, multi-functionalized triggered cell release based on aptamer and β-CD/graphene-modified surface and this multi-ways for cell catch-and-release on graphene modified surface also provides their potential biomedical application.

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“…Compared to other small--volume biomolecule sorting systems that rely on external electric fields, 38 IR, 39 magnetic fields, 7,40 require chemical modifications of the biomolecules of interest, 41 lead to only single--time use of a given set--up, 42 or require a series of sequential steps for the release of the biomolecule and/or reuse of the device, 32, 43 our system can exploit coupled chemistries in a microfluidic channel to yield both efficient (minimal steps) and effective (recovery of almost all of the target biomolecule from solution) output.…”

Section: Discussionmentioning

confidence: 99%