Aptamer-mediated DNA concatemer functionalized magnetic nanoparticles for reversible capture and release of circulating tumor cells

Zhang, Yating; Dong, Min; Xu, Panpan; Cai, Jiahui; Liu, Shi-He; Gao, Ya-Biao; Wang, Libin; Li, Jian; Hong, Jiasheng; Wang, Jidong; Wang, Desong

doi:10.1016/j.colsurfb.2022.112733

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…Using ImagJ (release 1.53 m 28) [34], single images from respective fluorescence channels were stacked into 3D images, split into their different color components and only the frame with the relevant color was saved. CellProfiler 4.1.3 [35] based pipeline was developed for feature extraction from single-colored 3D images.…”

Section: Feature Extractionmentioning

confidence: 99%

An Image-Based Identification of Aggressive Breast Cancer Circulating Tumor Cell Subtypes

Kamal

Wang

Plummer

et al. 2023

Cancers

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Using previously established CTC lines from breast cancer patients, we identified different morphometric subgroups of CTCs with one of them having the highest tumorigenic potential in vivo despite the slowest cell proliferation in vitro. This subgroup represents 32% of all cells and contains cells with small cell volume, large nucleus to cell, dense nuclear areas to the nucleus, mitochondria to cell volume ratios and rough texture of cell membrane and termed “Small cell, Large mitochondria, Rough membrane” (SLR). RNA-seq analyses showed that the SLR group is enriched in pathways and cellular processes related to DNA replication, DNA repair and metabolism. SLR upregulated genes are associated with poor survival in patients with ER+ breast cancer based on the KM Plotter database. The high tumorigenic potential, slow proliferation, and enriched DNA replication/repair pathways suggest that the SLR subtype is associated with stemness properties. Our new findings provide a simple image-based identification of CTC subpopulations with elevated aggressiveness, which is expected to provide a more accurate prediction of patient survival and therapy response than total CTC numbers. The detection of morphometric and transcriptomic profiles related to the SLR subgroup of CTCs also opens opportunities for potential targeted cancer treatment.

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Section: Feature Extractionmentioning

confidence: 99%

An Image-Based Identification of Aggressive Breast Cancer Circulating Tumor Cell Subtypes

Kamal

Wang

Plummer

et al. 2023

Cancers

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show abstract

“…AuNPs can also be combined with biological molecules (e.g. DNA) for applications in biomedical and bioanalytical devices [33] , [34] , [35] , [36] , [37] , [38] . For instance, it is possible to add ligands onto the surface of the AuNPs, allowing specific links with biomarkers of interest, increasing sensor selectivity [39] , [40] , [41] .…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles

Catunda

Prado

Oliveira

et al. 2023

Electrochimica Acta

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Size effect of dynamic bioactive magnetic particles on regulated isolation of tumor cells

Qin,

Bai,

Yang

et al. 2024

Applied Surface Science

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Aptamer-mediated DNA concatemer functionalized magnetic nanoparticles for reversible capture and release of circulating tumor cells

Cited by 7 publications

References 41 publications

An Image-Based Identification of Aggressive Breast Cancer Circulating Tumor Cell Subtypes

An Image-Based Identification of Aggressive Breast Cancer Circulating Tumor Cell Subtypes

SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles

Size effect of dynamic bioactive magnetic particles on regulated isolation of tumor cells

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