Aptamer-incorporated hydrogels for visual detection, controlled drug release, and targeted cancer therapy

Li, Jun; Liu, Huixia; Kang, Houjun; Donovan, Michael; Zhu, Zhi; Tan, Weihong

doi:10.1007/s00216-011-5414-4

Cited by 52 publications

(37 citation statements)

References 57 publications

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“…An acrydite DNA has a similar reactivity as free acrylamide monomers, allowing a high incorporation efficiency [43]. While the field of DNA-functionalized hydrogels began in 1996 [44], most of the developments have occurred in the past ten years [45][46][47][48]. In this article, we only provide a concise review of recent developments in visual detection using DNA-functionalized hydrogels.…”

Section: Dna Attachmentmentioning

confidence: 99%

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Khimji

Kelly

Helwa

et al. 2013

Methods

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Section: Dna Attachmentmentioning

confidence: 99%

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Khimji

Kelly

Helwa

et al. 2013

Methods

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“…These structural changes can stimulate specific physical or chemical interactions within hydrogel systems or circumambient solution systems. Entrapment of drugs [89,90], nanoparticles [91][92][93], and dyes [57,58] has greatly expanded the applications of aptamer-based hydrogels [94], allowing capabilities such as visual detection [56-58, 62, 95, 96], controlled drug release [97][98][99][100][101], and cancer therapy [89,90,102]. Furthermore, the availability of a wider range of aptamers will make aptamer-based hydrogel systems even more versatile in the future.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Hydrogels and Their Applications

Lü

et al. 2015

Aptamers Selected by Cell-Selex for Theranostics

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Hydrogels are water-retainable materials that can absorb a large amount of water. Different stimuli can be used to stimulate the hydrogels with a variety of physical and chemical changes, thus leading to numerous applications in bioanalysis and biomedicine. Aptamers are special types of single-stranded DNA generated by a process called systematic evolution of ligands by exponential enrichment (SELEX). They are able to specifically recognize a wide range of targets which vary from ions, small molecules, to proteins, and even whole cells. Aptamer incorporation has greatly expanded the applications of hydrogels. Due to their unique properties, such as biocompatibility, selective binding, and molecular recognition, these aptamer-based hydrogels can be utilized for target-responsive hydrogel engineering. In this chapter, we discuss a variety of applications of aptamer-based hydrogels, especially in sensing, target capture and separation, control of target release and in vivo applications.

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“…Measuring the partition coefficient of CBB into poly(HEMA)-based hydrogels lends itself to application in drug and biomolecule loading. Negatively charged biomolecules having potential applications in bioactive p(HEMA) hydrogels include cell adhesive factors [30], heparin [31], DNA aptamers [32], plasmids [33], and antimicrobial peptides [34,35]. The primary objective of this work was to explore the drug loading potential of novel, biomimetic p(HEMA)-based hydrogels by determination of the partitioning coefficient using CBB as a drug surrogate.…”

Section: Introductionmentioning

confidence: 99%

Partitioning of coomassie brilliant blue into DMAEMA containing poly(HEMA)-based hydrogels

Kotanen

Janagam

Idziak

et al. 2015

European Polymer Journal

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a b s t r a c tThe loading and release of anti-inflammatory drugs from hydrogel-coated implantable devices is a demonstrated approach in mitigating the inflammatory response of implantable devices. Poly(2-hydroxyethyl methacrylate)-based hydrogels possessing ionizable 2-(dimethylamino)ethyl methacrylate (DMAEMA) and cross-linked with varying concentrations (1, 3, 5, 7, 9 and 12 mol%) tetra(ethylene glycol) diacrylate (TEGDA) were synthesized and studied for their degree of hydration, glass transition temperature and partitioning of zwitterionic Coomassie Brilliant Blue (CBB), a zwitterionic drug surrogate known to bind to amines. Using UV-Vis spectroscopy, the partition coefficient of CBB within the hydrogels was found to average 6.69 ± 0.12 but to rise monotonically from 6.54 (1 mol%) to 6.84 (12 mol%) as a function of TEGDA mol% or cross-link density in accord with a monotonic reduction of the degree of hydration. The absence of DMAEMA was found to reduce the partition coefficient of CBB from 6.5 to 4.1. Both formulations, with and without DMAEMA (pK a = 7.5), showed an independence of pH over the physiologically relevant range, pH 5-pH 9, confirming that this difference was not due to Henderson-Hasselbalch ionization of the pendant group. The presence of DMAEMA resulted in a 1.44 fold increase in CBB loading confirming complex formation. The absence of DMAEMA still resulted in considerable partitioning of CBB into the hydrogel suggesting that the affinity between CBB and DMAEMA may not be the only relationship controlling the partition coefficient.

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Aptamer-incorporated hydrogels for visual detection, controlled drug release, and targeted cancer therapy

Cited by 52 publications

References 57 publications

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Aptamer-Based Hydrogels and Their Applications

Partitioning of coomassie brilliant blue into DMAEMA containing poly(HEMA)-based hydrogels

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